Abstract: Gastric cancer is a significant global public health challenge, causing a substantial disease burden. RNA alternative splicing（AS） is a critical gene expression regulation mechanism prevalent in higher eukaryotes. Through differential splicing patterns during the conversion of precursor mRNA to mature mRNA, a single gene can produce multiple splicing isoforms, thereby increasing the diversity and complexity of both transcriptomes and proteomes to meet the diverse physiological needs of an organism. RNA⁃AS dysregulation has been found to be associated with nearly all tumor types, contributing directly or indirectly to gastric cancer initiation, progression, drug resistance, and prognosis. Additionally, RNA⁃AS⁃related genetic variations play a crucial role in gastric cancer. This article systematically reviews research advances in the regulatory mechanisms of RNA⁃AS, as well as the impact of its dysregulation and related genetic variantions on gastric cancer, aiming to provide novel theoretical foundation and research perspectives for both RNA⁃AS⁃related further investigations and precision medicine strategies of gastric cancer.

Key words: Gastric cancer, RNA?alternative splicing, Splicing factor, Genetic variation, Biomarker