Abstract: Objective To detect the response of hepatic tissue to ischemic reperfusion and the relationship between the expression of HSP70 mRNA and apoptosis.And then to provide a theoretical basis for selecting effective pretreatment method,inhibiting apoptosis and reducing the hepatic injury by ischemic reperfusion.Methods liver tissue(10g for each) was taken around incisal margin before the blockage of porta hepatis,the open of porta hepatis and before the closure of abdominal cavity, respectively.In situ hybridization was used to detect the expression of HSP70 mRNA.Terminal deoxynucleotidyl transferase mediated dUTP nick end labeling was used to detect the apoptosis in hepatic tissue.Results①The numbers of positive cell of HSP70 mRNA expression in the pre-blockage of porta hepatis group,the open of porta hepatis group and the pre-closure of abdominal cavity group was 1.90±0.84,24.4±5.7 and 35.1±5.1 respectively.Differences among these three groups were significant(P<0.05).②The AI(apoptosis index) of hepatic tissue in the pre-blockage of porta hepatis group,the open of porta hepatis group and the pre-closure of abdominal cavity group was(11.4±2.4)‰,(9.8±1.7)‰and(8.6±1.6)‰respectively.AI in the pre-blockage of porta hepatis group was more than that in the open of porta hepatis group and the preclosure of abdominal cavity group(P<0.05).AI in the open of porta hepatis group was more than that in the pre-closure of abdominal cavity group(P<0.05).Conclusions①There is little expression of HSP70 mRNA in the normal hepatic tissue, while there is high expression in hepatic ischemic reperfusion injury(HIRI).It suggests that there is some relationship between HSP70 and HIRI.②In the open of porta heptis group,there is a negative correlation between the increased expression of HSP70 mRNA and the decreased of AI,which suggests HSP70 has an effect on suppressing apoptosis.

Key words: Liver, Ischemic reperfusion, HSP70 mRNA