Abstract: Objective To construct a lentiviral vector for RNA interference of the inter-α-trypsin inhibitor H4 (ITIH4) gene. Methods A short hairpin RNA (shRNA) targeting the ITIH4 gene was designed based on the optimal siRNA sequence. The double-stranded DNAs obtained after annealing were ligated with the linearized lentiviral vector Psico using T4 DNA ligase and transformed into E. coli. The recombinant plasmid was extracted and identified by PCR and sequencing. The plasmid DNA of Psico-shRNA-ITIH4 was packaged into 293 T cells, and the fluid virus titer in the culture medium was determined. Results The sequence of recombinant lentiviral plasmid Psico-shRNA-ITIH4 was consistent with the sequence of the designed shRNA. The virus titer was approximately 9.5 × 10³ IU/ml. Conclusion The recombinant plasmid Psico-shRNA-ITIH4 was constructed successfully and provides an experimental basis for the further study of ITIH4.

Key words: Inter-α-trypsin inhibitor H4, Lentiviral vector, shRNA