Abstract:

Objective To investigate the effects of Valsartan（VAT） on myocardial cell damage induced by doxorubicin（DOX）. Methods  A model of DOX-induced myocardial cell damage was established by exposing H9C2 myocardial cells to different concen-trations of DOX（0.5，1，2，4 μmol/L）. Cell survival rate was detected by CCK8 colorimetry. Cell apoptosis rate and cell cycle change were detected by flow cytometry.  Results The cytotoxic effects of DOX on H9C2 cells were dose- and time-dependent for concentra-tions from 0.5 to 4 μmol/L during exposure times from 12 h to 24 h. Exposure to 1 μmol/L DOX for 24 h led to significant cytotoxic effects. The treated cells showed （75.5±5.6）% of the survival rate of control cells，and the rate of cell apoptosis was （11.94±2.07）% compared to（4.86±0.56）% in control cells（P＜0.05）. Pretreatment with 10 VAT μmol/L led to significantly higher survival rate ［（89.3±4.0）% vs （74.2±3.0）%，P＜0.01］，significantly lower apoptosis rate ［（11.01±3.17）% vs （13.15±6.07）%，P＜0.01］，and significantly lower ratio of cells in G₀ /G₁ phase ［（50.3±1.2）% vs （69.21±2.03）%，P＜0.05］. Conclusions VAT can mitigate DOX-induced damage to myocardial cells，and the protective effect may be related to the suppression of apoptosis.

Key words: Valsartan, Doxorubicin, Myocardial injury, Apoptosis, Antagonism