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25 September 2011, Volume 3 Issue 3 Previous Issue    Next Issue

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Expression of TIZ mRNA in ovarian cancer cell lines and the construction of RNA interference vectors ZHAO Bing-Bing, ZHANG Wei, LI Li, YANG Zhi-Jun 2011, 3 (3):  187-191.  doi: 10.3969/j.issn.1674-5671.2011.03.02 Abstract ( 534 )   PDF (1638KB) ( 596 )   Save Objective To detect the level of TIZ mRNA in ovarian cancer cell lines and to construct the vector of TIZ mRNA interference (RNAi).Methods The expression levels of TIZ mRNA in several ovarian cancer cell lines were detected.The cell lines included SKOV3，SKOV3-cisplatin resistance，SKOV3-carboplatin resistance，A2780，A2780-cisplatin resistance and HO8910.Five pairs of TIZ siRNA were designed according to the sequence of TIZ mRNA in Genebank.The siRNAs were transfected by liposome to down-regulate the expression of TIZ mRNA in ovarian cancer cell lines.TIZ mRNA and β-actin mRNA expression plasmids were construct red by using pTG19-T plasmid，and their standards were prepared.The real-time quantitative polymerase chain reaction (QRT-PCR) was used to detect the efficiency of TIZ siRNA and then to select the best one.The pGPU6/GFP/Neo vector and plasmid DNA sequencing method was used to build pGPU6/GFP/Neo-siRNA-TIZ-573 restructured plasmid.Results TIZ mRNA expressed in all ovarian cancer cells except HO8910.siRNA TIZ-573 down regulated 60% of TIZ mRNA expression.The vector TIZ RNAi pGPU6/GFP/Neo-TIZ-573 was constructed successfully.Conclusions Most ovarian cancer cell lines express TIZ mRNA.siRNA-TIZ-573 is the best segments that can down regulate TIZ mRNA.The recombinanted plasmid GPU6/GFP/Neo-TIZ-573 can be used for the next experiments. Related Articles | Metrics

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Clinical signification of chromosomal aberration in hepatocellular carcinoma determined by comparative genomic hybridization XIAO Kai-Yin, PENG Min-Hao, QIN Quan-Lin, GUI Wen-Bo, QIN Xiao, PENG Tao, GUO Ya, CHEN Bin, LU Jing-Ning, LI Le-Qun 2011, 3 (3):  192-193.  doi: 10.3969/j.issn.1674-5671.2011.03.03 Abstract ( 528 )   PDF (828KB) ( 789 )   Save Objective To screen chromosomal aberration in hepatocellular carcinoma (HCC) and to explore the clinical signification.  Methods  Chromosomal aberration was detected by comparative genomic hybridization (CGH) in 25 patients with HCC，and the correlation between chromosomal aberration and clinical parameters were analyzed. Results All patients presented certain aberrations in copy number of chromosomal DNA. The amplification of copy number was commonly detected in chromosome 1q (72.0%)，1p (64.0%)，2q (48.0%)，2q (48.0%)，5q (48.0%) and 7q (44.0%)，while the loss of copy number was often found in chromosome 4q (48.0%)，16p (48.0%)，8p (40.0%) and 17p (36.0%).  The amplification of 17p and 18p as well as the loss of 8p，13q，11q and 8q showed significant correlation with some clinical parameters. Conclusions Chromosomal aberrations occur frequently in HCC. Some of the nonrandom chromosomal alterations may relate to the development，biological behavior and prognosis of HCC. Related Articles | Metrics

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CO2-insufflation on expression of CDK9 mRNA of cervical cancer cell Hela PAN Ling-Hui, LIN Fei, LI Li, HUANG Bing 2011, 3 (3):  196-200.  doi: 10.3969/j.issn.1674-5671.2011.03.04 Abstract ( 536 )   PDF (1116KB) ( 421 )   Save Objective To investigate the effect of CO2-insufflation on expression of CDK9 mRNA of cervical cancer cell line Hela, and to analyze the role of CO2 in growth and metastasis of Hela. Methods Hela cells were randomly divided into two study groups (A and B) and one control group (C). The cells in group A were exposed to CO2 with 8 mmHg and incubated for 24 hours,the cells in group B were exposed to CO2 with 8 mmHg and incubated for 120 hours, the cells in group C were not exposed to CO2.Real-time PCR was used to detect the expression of CDK9 mRNA of Hela cells collected from each group.ResultsT he expression of CDK9 mRNA of Hela cells in the two study groups was lower than that in control group (P＜0.05),and the expression of CDK9 mRNA of Hela cells in group B was lower than that in group A (P＜0.01)Conclusions The expression of CDK9 mRNA can be down-regulated by CO2-insufflation.The expression of CDK9 mRNA decreased more obvious with longer incubation time Related Articles | Metrics

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EGFR mutations among 163 patients of non-small cell lung cancer with Guangxi Zhuang nationality LI Ji-Lin, LIN Shu-Han, TANG Yan-Ping, TAN Xiao-Yu, LI Yuan-Dong, DENG Wei, 欧Chao , RONG Min-Hua, SHEN Xiao-Yun, ZHANG Li-Tu 2011, 3 (3):  200-203.  doi: 10.3969/j.issn.1674-5671.2011.03.05 Abstract ( 543 )   PDF (820KB) ( 567 )   Save Objective  To investigate mutations of gene epidermal growth factor receptor (EGFR) in the patients of non-small cell lung cancer (NSCLC) who were Guangxi Zhuang nationality. Methods 163 cases of NSCLC tissue were enrolled in this study. Mutations  of EGFR exons 18，19，20 and 21 were detected by amplification refractory mutation system (ARMS). The relation between EGFR mutation and clinical characters were analyzed，and they were compared with the reported data from other eight domestic regions. Results Mutations were found in 73 cases，with an incidence of 44.8% which was significantly higher than other eight domestic regions (30.0%，P＜0.05).The mutations were mainly in exon 19 and 21，each of them accounted for 38.4% of total.Mutations in adenocarcinoma and adenosquamous carcinoma accounted for 80.8% of total.Mutation rate in female (57.7%) was significantly higher than that in male (38.7%). Conclusions  Incidence of EGFR mutation in NSCLC patient with Zhuang nationality is higher than that in other regions of China. Mutations in exons 19 and 21 are more common. Female patient，patients with adenocarcinoma or adenosquamous carcinoma may be the appropriate crowd to be treated with EGFR tyrosine kinase inhibitor. Related Articles | Metrics

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Subtype analysis of human papillomavirus among the cervix-infected women in Nanning area of Guangxi GAO Kun, LI Li, YANG Mei 2011, 3 (3):  203-206.  doi: 10.3969/j.issn.1674-5671.2011.03.06 Abstract ( 450 )   PDF (821KB) ( 702 )   Save Objective To analyze the situation and distribution of cervical human papillomavirus (HPV) infection among women in Nanning area of Guangxi. Methods Through clinic opportunistic screening，1 250 women received HPV genotypes test with gene chip technology. Result The positive rate of HPV DNA was 31.36%(392/1250).The major infected subtypes were the low-risk ones，and the descending order of prevalence was subtype 6，11 and 43. The descending order of prevalence among the high-risk subtypes was 16，58，52 and 18. Single high-risk subtype infection was common，followed by single low-risk subtype infection.Among the multiple-subtype infections，double infection with low-and high-risk subtype was common，while triple or quadruple infection was rare.Women aged ≤25 years old accounted for 42.35% of infection，followed by 25 to 34 years old. Conclusions Distribution of HPV subtypes is regional. Detection of HPV subtypes will help to predict the outcome of infection，guide clinical treatment and follow-up. Related Articles | Metrics

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Differential expression of SLC30A9 in human breast cancer and its clinical significance LIAO Yan, LIN Shu-Han, LI Dan-Rong, LI Ji-Lin, TANG Dong-Ping, TANG Kai, ZHANG Li-Tu 2011, 3 (3):  206-209.  doi: 10.3969/j.issn.1674-5671.2011.03.07 Abstract ( 512 )   PDF (908KB) ( 530 )   Save Objective To investigate the expression of gene solute carrier family 30 member 9 (SLC30A9) in human breast cancer，and to explore the correlation between expression of SLC30A9 mRNA and breast cancer. Methods 30 cases of surgically removed breast cancer tissue and their adjacent tissue，10 cases of normal breast tissue and 20 cases of benign breast lesion were enrolled in this study. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of SLC30A9 mRNA，and its clinical significances were analyzed. Results All the 90 samples expressed SLC30A9 mRNA. The average optical density ratios of SLC30A9 mRNA in the cancerous tissues，cancer-adjacent tissues，normal breast tissues and benign breast lesion tissues were 0.443±0.247，0.427±0.253，0.405±0.209 and 0.547±0.190 respectively，without significant difference (P＞0.05). Five of the 30 (16.7%) cancer tissues showed differential expression，among which 3 down-regulated and 2 up-regulated comparing to the normal counterparts. The average optical density rations of SLC30A9 mRNA were not statistically associated with age，clinical stages，lymphonode metastasis，distant metastasis and histopathological type (P＞0.05). Conclusion SLC30A9 expresses in a variety of breast tissues but only 16.7% cases are differential expression，suggestion it may not be the dominant gene for carcinogenesis of breast cancer. Related Articles | Metrics

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Relationship between methylation of MGMT gene in plasma of patients with diffuse large B-cell lymphoma and efficacy of chemotherapy KANG Ma-Fei, LUO Mei-Qing, LIU Ying, LIAO Li-Li, CHEN Ying 2011, 3 (3):  210-213.  doi: 10.3969/j.issn.1674-5671.2011.03.08 Abstract ( 454 )   PDF (866KB) ( 420 )   Save Objective To detect the methylation of gene O6-methylguanine DNA methyltransferase (MGMT) in peripheral plasma of patients with diffuse large B-cell lymphoma (DLBCL)，and to evaluate the relationship between methylation of MGMT and efficacy of chemotherapy on DLBCL.Methods  Before and after chemotherapy，nestedmethylation specific PCR (nMSP) was performed for detecting methylation of MGMT in plasma from DLBCL patients who were treated with CHOP.Result Methylation of MGMT was found in 63.3% (19/30) of patients with DLBCL.The response rates were 100% (19/19) in patients with methylation of MGMT and 72.7% (8/11) in patients with non-methylation of MGMT，and the difference between two groups was statistically significant (P=0.041).The ratios of resistance to chemotherapy were 10.5% (2/19) in patients with methylation of MGMT and 54.5% (6/11) in patients with non-methylation of MGMT，and the difference between two groups was statistically significant (P=0.028).Conclusion Methylation of MGMT in peripheral plasma of DLBCL patients might be a biomarker of response or resistant to chemotherapy with alkylating agents based regimens. Related Articles | Metrics

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The impact of setup errors on treating head and neck cancer with intensity modulated radiation therapy ZHENG Zhuo, CHEN Chuan-Ben, CHEN Li-Sha, ZHANG Yu, FEI Zhao-Dong 2011, 3 (3):  214-217.  doi: 10.3969/j.issn.1674-5671.2011.03.09 Abstract ( 544 )   PDF (1085KB) ( 777 )   Save Objective To study the impact of setup errors on treating head and neck cancer (HNC) with intensity modulated radiation therapy (IMRT).Methods Patients were scanned with KV-CBCT to adjust position before treatment. The setup errors including translational errors and rotation errors were gain from XVI (X-Ray volume Image) matched with original planed CT image. The setup errors were input to pinnacle treatment planning system，and then the treatment plans with setup errors were recalculated. The dose distribution and dose volume histogram (DVH) of tumor and critical structure were compared with original treatment plan.The evaluation indicators included mean dose (Dm) of planning target volume of primary tumor (GTV-T-P)，dose of 95% volume (D95)，dose of 1% volume and 1cc volume of spinal cord and brainstem，mean dose of bilateral parotid gland and the dose volume received more than 30Gy.Statistical analysis was performed by using SPSS 16.0.Results The mean translational error and rotation error along X，Y and Z axis were (1.02±0.83) mm，(1.28±1.08) mm and (1.27±1.06) mm respectively，and (0.79±0.67) degree，(1.00±0.82) degree and (0.73±0.68) degree respectively. The dose differences among brainstem，spinal cord and parotid gland were statistically significant. The difference of GTV-T-P (Dm) was not statistically significant，while D95 was significant different but the average rate of change was 2.18% only.Conclusion Setup error of IMRT affect dose distribution of both target and normal tissue，and the effect is greater on normal tissue than that on target tissue. Related Articles | Metrics

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Safety and feasibility of concurrent chemotherapy with thoracic three-dimensional radiotherapy on patients with non-small cell lung cancer at stage IV ZHANG Bo, LU Bing, SU Sheng-Fa, HU Yin-Xiang, 欧Yang-Wei-Wei , LI Hui-Qin, WANG Gang, LONG Jin-Hua 2011, 3 (3):  218-222.  doi: 10.3969/j.issn.1674-5671.2011.03.10 Abstract ( 535 )   PDF (1422KB) ( 646 )   Save Objective To evaluate the safety and feasibility of concurrent chemotherapy with thoracic three-dimensional radiotherapy (TTDRT) on patients with non-small cell lung cancer (NSCLC) at stage IV.Methods  From Jan 2003 to July 2008，201 patients with NSCLC at stage IV entered the study.The median age of the patients was 60 years.All patients received platinum-based chemotherapy，with the median of 3 cycles.The median radiation dose to PTV of primary tumor was 60.3Gy，while 138 patients received TTDRT on metastatic foci.The treatment-related gastrointestinal and hematological toxicity were scored.Results  19 patients who received only one cycle of chemotherapy were not included in survival analysis but included in toxicity analysis.The rates of patients completed the full treatment，adequat radiotherapy，systemic chemotherapy，defect treatment and no treatment were 32%，14%，14.3%，30.3% and 9%，respectively.The median survival time of patients received 4 to 5 cycles of chemotherapy and DTPTV≥63Gy of TTDRT was 16.1 months，which was significantly longer than other treatments (χ2=25.369，P=0.000).45% of all patients had grade 2~3 gastrointestinal toxicity.The rates of grade 3~4 WBC，PLt and Hb toxicity were 35%，18% and 15%，respectively.The rates of grade 2~3 radiation pneumonitis and esophagitis were 9.5% and 13.4% respectively.The median survival times of patients with improved KPS and decreased KPS≥10 cases after treatment were 12 months and 4 months respectively (χ2=78.452，P=0.000).Cox regression analysis showed the improved KPS after treatment was an independent factor for survival (P=0.000).Conclusions  Concurrent chemotherapy with TTDRT on patients with NSCLC at stage IV is safe and tolerable.4 to 5 cycles of chemotherapy simultaneously with DTPTV ≥ 63Gy of TTDRT may improve survival time. Related Articles | Metrics

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Salivary gland scintigraphy in evaluating parotid function of patients with nasopharyngeal carcinoma after radiotherapy HUANG Miao, XIAO Guo-You, LI Dang-Sheng, YAO Zhong-Qiang, DENG Li-Yan 2011, 3 (3):  222-225.  doi: 10.3969/j.issn.1674-5671.2011.03.11 Abstract ( 446 )   PDF (826KB) ( 825 )   Save Objective To study the feasibility of salivary gland scintigraphy in evaluating parotid function of patients with nasopharyngeal carcinoma (NPC) after radiotherapy. Methods Forty patients with NPC who underwent intensity-modulation radiation therapy  30cases(IMRT) or conventional radiotherapy 10 cases were observed.The level of mouth dryness and parotid function before IMRT，at the end of IMRT and 5 month after IMRT were compared by each other.Results Different degrees of mouth dryness were common at the end of IMRT among the patients，but some of them recovered significantly 5 month after IMRT.The parotid scintigraphy signs between before and after IMRT were different，both of uptake index (UI) and excretion index (EI) after IMRT were significant lower than those of before IMRT (P＜0.05).However，the UI and EI of the patients treated with IMRT were significantly higher than those treated with conventional radiotherapy (P＜0.05).Conclusions IMRT can effectively protect parotid function.Salivary gland scintigraphy is sensitive and feasible for monitoring parotid function of NPC patients after radiotherapy. Related Articles | Metrics

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Anesthesia of experimental dogs for total laryngectomy and laryngeal reconstruction CHEN Mei-Qiu, XU Zhi-Wen, WEN Wen-Sheng, ZHENG Shi-Xing, LIANG Jie-Zhen, ZENG Man-Li 2011, 3 (3):  225-228.  doi: 10.3969/j.issn.1674-5671.2011.03.12 Abstract ( 441 )   PDF (816KB) ( 576 )   Save Objective To explore a proper anesthesia method for laryngeal surgery on experimental dogs.Methods  Thirteen adult dogs undergoing total laryngectomy and laryngeal function reconstruction were divided randomly into two groups.Animals in group A were treated with 3% pentobarbital sodium (1ml/kg，intraperitoneal injection) only.Animals in group B were treated with 5% pentobarbital sodium (0.25ml/kg，intramuscular injection) combined with Sumianxin II (0.05ml/kg，intramuscular injection).Results The treatment in group A showed the anesthesia induction time was（19.7±6.2）min，the maintenance of first anesthesia time was（119.6±34.6）min.It needed several more injections to keep anesthesia during the surgery and had poor postoperative recovery.The treatment in group B showed the induction time was（5.5±1.8）min，the maintenance of anesthesia time was（112.5±24.3）min.Only one more injection with 1/2 dose of the first infection was needed，which prolonged the maintenance of anesthesia time to at least（169.8±20.2）min.The dogs in this group reanimated soon after operation.Between group A and group B，the induction time was significantly different (P＜0.05) while the maintenance time of first anesthesia was not significantly different (P＞0.05).Both of the times of additional injection and the ratio of actual amount vs suggested amount of pentobarbital were significantly different (P＜0.05)Conclusions Pentobarbital sodium combined with Sumianxin appears to be a satisfactory method to anaesthetize dogs for the operation of total laryngectomy and laryngeal function reconstruction.It can decrease induction time，reduce the time and dose of additional anesthetics，and obtain more effective anesthesia and rapid recovery. Related Articles | Metrics

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Long-term efficacy of three-dimensional conformal radiation on treating primary liver carcinoma LIANG Xia, ZHU Xiao-Dong, LI Ling, CHEN Long, LIANG Shi-Xiong, HUANG Qi-Fang 2011, 3 (3):  229-231.  doi: 10.3969/j.issn.1674-5671.2011.03.13 Abstract ( 434 )   PDF (838KB) ( 668 )   Save Objective To evaluate the therapeutic effects of three-dimensional conformal radiation (3DCRT) on treating unresectable primary liver cancer (PLC).Methods From April 1999 to July 2007，a total of 153 patients with PLC who could not receive surgical therapy and were treated with 3DCRT alone were reviewed.Results The overall efficiency was 37.3%.The l-，2- and 3-year survival rates were 38.0%，25.0% and 20.0%，respectively.Conclusion 3DCRT is effective and feasible for treating unresectable PLC. Related Articles | Metrics

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Magnetic resonance imaging manifestations of radiation-induced brainstem injury in patients with nasopharyngeal carcinoma after radiotherapy LIU Min, LIANG Shi-Xiong, LIAO Jin-Yuan, JIN Guan-Qiao 2011, 3 (3):  232-235.  doi: 10.3969/j.issn.1674-5671.2011.03.14 Abstract ( 566 )   PDF (994KB) ( 787 )   Save   Objective To analyze the magnetic resonance imaging (MRI) manifestation characteristics of radiation-induced brainstem injury in patients with nasopharyngeal carcinoma (NPC) after radiotherapy.Methods Brain MRIs from 37 patients with NPC who developed radiation-induced brainstem injury after radiotherapy were reviewed.The study analyzed imaging sequences included T1-weighted image (T1WI)，T2-weighted image (T2WI)，fluid attenuated inversion recovery (FLAIR) and enhanced scanning imaging to define the site，range，signal strength and enhanced form of the lesions with radiation-induced brainstem injury. Results Among the 37 patients，19 had lesions in pons; 1 in midbrain; 3 in medulla oblongata; 6 in pons and midbrain; 5 in pons and medulla oblongata; 3 in pons，midbrain and medulla oblongata. All lesions showed hypo-or iso-intense signal on T1WI，and hyper-intense signal on T2WI.With contrast enhanced scanning，9 cases did not show enhancement，28 cases showed marked enhancement which included 12 cases with homogenous patchy enhancement and 16 cases with heterogeneous ringed and patchy enhancement. Conclusion MRI can clearly show the lesions of radiation-induced brainstem injury in NPC patients after radiotherapy，which is available for clinical diagnosis. Related Articles | Metrics

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Impact of blood supply on treating metastatic liver cancer with transcatheter arterial chemoembolization SHEN Fei, MAO Li-Ping, SUN Ya-Jun 2011, 3 (3):  235-237.  doi: 10.3969/j.issn.1674-5671.2011.03.15 Abstract ( 408 )   PDF (962KB) ( 536 )   Save Objective To investigate the impact of blood supply on treating metastatic liver cancer with transcatheter arterial chemoembolization (TACE).Methods Clinical efficacy and survival rate were analyzed on 34 cases of metastatic liver cancer that were treated by TACE.Results Metastatic liver cancer was lack of blood supply-based.The values of CR+PR in hypervascular group and lack of blood supply group were 66.7% (8/12) and 31.8% (7/22) respectively，and the difference between the two groups was statistically significant (P＜0.05).The survival rates of 6-and 12-month after metastasis in hypervascular group were 100% and 75.0% respectively，while in lack of blood supply group were 81.8% and 40.9% respectively.The median survival periods after metastasis in hypervascular group and lack of blood supply group were 21.6 and 13.5 months respectively，and the difference between the two groups was statistically significant (P＜0.05).Conclusion TACE is significantly more effective for treating metastatic liver cancer with rich blood supply. Related Articles | Metrics

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Relation of V20 and MLD to radiationinduced lung injury JIANG Xue-Chao, CUI Hong-Xia, SONG Hong-Qiang 2011, 3 (3):  238-240.  doi: 10.3969/j.issn.1674-5671.2011.03.16 Abstract ( 585 )   PDF (809KB) ( 676 )   Save Objective To investigate the relation of V20 and MLD to radiation-induced lung injury.Methods From Aug 2008 to Jun 2010，47 patients with lung cancer and met the study criteria were enrolled. All patients receive 3D conformal radiotherapy by 6MVX-ray，and the doses ranged between 50Gy to 70Gy. DVH was gained through 3-dimensional TPS. The radiation treatment planning was V20 ≤ 30% and the spinal cord volume was≤45Gy. Radiation-induced lung injury was diagnosed based on contrasted CT combined with clinical symptoms，and the extent of injury was evaluated according to RTOG grading criteria.Results The total incidence of radiation-induced lung injury was 31.9% (15/47). While the incidence of lung injury was higher in the group of V20＞25% (46.2%) than that in the group of V20≤25% (14.3%，P＜0.05)The incidence of lung injury was lower in the group of MLD≤15Gy (14.3%) than that in the group of MLD＞15Gy (57.9%，P＜0.05).  Conclusion The radiation-induced lung injury related significantly to V20 and MLD that could help to predict the injury. Related Articles | Metrics

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The value of mammography in evaluating the effect of neoadjuvant chemotherapy on patients with breast cancer HUANG Miao, SU Dan-Ke, LAI Shao-侣, HUANG Xiang-Yang, LI Qiang 2011, 3 (3):  241-242.  doi: 10.3969/j.issn.1674-5671.2011.03.17 Abstract ( 473 )   PDF (790KB) ( 576 )   Save Objective To explore the value of breast mammography in evaluating the effect of neoadjuvant chemotherapy (NAC) on patients with breast cancer.Methods Breast mammography images from 21 patients with breast cancer before and after NAC were collected，and the main changes of Xray signs were analyzed.Results After NAC，all of the 21 cases showed smaller tumor size，4 cases showed reduced mass-density locally，20 cases showed changes in tumor shape，9 cases showed reduced range of calcification，and 9 cases showed smaller axilla lymph nodes with reduced density.Conclusions Mammography findings are deferent between before- and after-NAC on patients with breast cancer. Mammography can be used to assess the efficacy of NAC. Related Articles | Metrics

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Clinical observation of microwave ablation with water circulation cooling system for treating advanced peripheral lung cancer LI Fa-Qiang, FENG Li-Dong, LI Ben-Quan, ZHANG Sheng, WANG Dao-Jun, LIU A-Hai, ZHAN Xiang-Cao, JIN Gui-Hong, WANG Yan-Xia 2011, 3 (3):  242-245.  doi: 10.3969/j.issn.1674-5671.2011.03.18 Abstract ( 443 )   PDF (815KB) ( 775 )   Save Objective To explore the feasibility and clinical effect of the microwave ablation technology with water circulation cooling system on treating advanced peripheral lung cancer. Methods 36 patients with inoperable advanced peripheral lung cancer were treated firstly with chemotherapy and radiotherapy. The residual tumors were then treated by microwave ablation with water circulation cooling system. Results  Operations were completed in 36 patients，the completion rate was 100%. 3 days after treatment，33 cases showed remission of cough，sputum，hemoptysis，chest pain and other symptoms，the remission rate was 91.67%3~6 months after treatment，with enhanced CT on 42 tumors，36 (85.71%) showed complete necrosis absorption，4 (9.52%) expressed as most (≥ 90%) necrotic absorption，2 showed 50% to 70% necrotic absorption. Tumor diameters were reduced 2.5 to 5cm，with the average of 4cm. 31 (86.11%) cases obtained complete remission，4 (11.11%) cases obtained partial remission and 1 (2.78%) case had no change. The total remission rate was 97.22% and clinical benefit rate was 100%，the overall survival time was 10 to 46 months，and the average survival time was 28 months.ConclusionMicrowave ablation technique with water circulation cooling system can be used as a palliative treatment for inoperable advanced peripheral lung cancer. Related Articles | Metrics