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中国癌症防治杂志

中国癌症防治杂志 ›› 2025, Vol. 17 ›› Issue (4): 408-415.doi: 10.3969/j.issn.1674-5671.2025.04.02

• 新技术新方法专栏 • 上一篇    下一篇

碳基负载钌纳米颗粒协同光热-化学动力学疗法抑制乳腺癌细胞增殖与迁移

  

  1. 广西医科大学附属肿瘤医院实验研究部
  • 出版日期:2025-08-25 发布日期:2025-09-11
  • 通讯作者: 张维青，E-mail:zhangweiqing2008@hotmail.com
  • 基金资助:
    广西科技计划项目（桂科AD22035044）；广西医科大学高水平创新团队及杏湖学者计划；广西医科大学附属肿瘤医院引进高层次人才

Ru nanoparticles⁃carbon support synergize photothermal⁃chemodynamic therapy to inhibit the proliferation and migration of breast cancer cells

  • Online:2025-08-25 Published:2025-09-11

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摘要： 目的 探讨碳基底负载钌纳米颗粒（Ru nanoparticles⁃carbon support, Ru⁃NPs/C）介导的化学动力学疗法（chemodynamic therapy, CDT）和光热疗法（photothermal therapy, PTT）对乳腺癌细胞增殖与迁移的影响及其作用机制。方法 通过模板牺牲辅助热解法合成Ru⁃NPs/C，并运用X射线衍射、透射电子显微镜、X射线光电子能谱、紫外-可见分光光度计和红外热像仪对Ru⁃NPs/C的结构与催化性能进行表征。通过MTT实验和细胞划痕实验评估Ru⁃NPs/C和近红外光（near⁃infrared,NIR）分别单独使用或联用对4T1细胞增殖与迁移的影响；采用溶血实验评价其生物相容性；使用2',7'-二氯二氢荧光素二乙酸酯（DCFH⁃DA）荧光探针检测细胞内活性氧（reactive oxygen species, ROS）水平。结果 成功合成Ru⁃NPs/C，其中钌（Ru）纳米颗粒在碳载体上高度分散， X射线光电子能谱分析进一步揭示了Ru0⁃Ru3+混合价态形式存在。Ru⁃NPs/C可同时模拟过氧化物酶、过氧化氢酶及谷胱甘肽氧化酶样活性，并表现出显著的光热响应。与对照组及各单一处理组相比，Ru⁃NPs/C与NIR联用可显著增加ROS水平，抑制4T1细胞增殖与迁移（均P<0.01）。Ru⁃NPs/C对正常乳腺细胞MCF⁃10A无明显毒性，在150 µg/mL浓度下溶血率仍低于5%，显示出良好的生物相容性。结论 Ru⁃NPs/C具有高效的多酶催化性能，在NIR激发下可高效诱导ROS生成，进而抑制乳腺癌细胞的增殖与迁移，展现出良好的协同治疗潜力。

关键词: 乳腺癌, 钌基纳米颗粒, 化学动力学疗法, 近红外光热治疗, 活性氧

Abstract: Objective To investigate the effects and underlying mechanisms of Ru nanoparticles⁃carbon support (Ru⁃NPs/C) in mediated chemodynamic therapy (CDT) and photothermal therapy (PTT) on the proliferation and migration of breast cancer cells. Methods The Ru⁃NPs/C were synthesized via a template⁃sacrificed assisted pyrolysis method, and their structural and catalytic properties were characterized by X⁃ray diffraction (XRD), transmission electron microscopy (TEM), X⁃ray photoelectron spectroscopy (XPS), ultraviolet⁃visible spectrophotometry, and infrared thermography. The effects of Ru⁃NPs/C, both independently and in conjunction with near⁃infrared (NIR) radiation, on the proliferation and migration of 4T1 cells were assessed using the MTT assay and scratch test. The biocompatibility was evaluated via a hemolysis assay, and intracellular reactive oxygen species (ROS) level was measured using the 2',7'⁃DCFH⁃DA fluorescent probe. Results Ru⁃NPs/C was successfully synthesized with Ru nanoparticles were highly dispersed on the carbon support. XPS analysis further revealed the co⁃existence of Ru in mixed valence states (Ru0 and Ru3+). Ru⁃NPs/C simultaneously mimicked robust peroxidase (POD)⁃like, catalase (CAT)⁃like, and glutathione oxidase (GSH⁃OXD)⁃like catalytic activities while exhibiting significant photothermal responsiveness. Compared to control and individual treatment groups, the combination of Ru⁃NPs/C with NIR group markedly increased ROS levels and inhibited the proliferation and migration of 4T1 cells (all P<0.01). Meanwhile, Ru⁃NPs/C demonstrated negligible cytotoxicity to normal cells MCF⁃10A, with a hemolysis rate below 5% even at 150 µg/mL, exhibiting excellent hemocompatibility. Conclusions Ru⁃NPs/C possess efficient multi⁃enzyme catalytic properties. Under NIR stimulation, effectively generate ROS to inhibit the proliferation and migration of breast cancer cells, demonstrating excellent potential for synergistic therapy.

Key words: Breast cancer； Ruthenium-based nanoparticles, Chemodynamic therapy, Near-infrared photothermal therapy, Reactive oxygen species

中图分类号: 

  • R737.9