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中国癌症防治杂志

中国癌症防治杂志 ›› 2025, Vol. 17 ›› Issue (5): 613-618.doi: 10.3969/j.issn.1674-5671.2025.05.13

• 论著 • 上一篇    下一篇

 C. leadbetteri 对舌鳞状细胞癌细胞系Cal27增殖、侵袭和迁移能力的影响

  

  1. 滨州医学院口腔医学院；266071 青岛 2.青岛市市立医院口腔科；青岛市中医医院（青岛市海慈医院）；山东第二医科大学口腔医学院；青岛市市立医院中心实验室
  • 出版日期:2025-10-25 发布日期:2025-12-03
  • 通讯作者: 王莉莉 E-mail: allyking114@126.com；周建华 E-mail: zhoujhqd@126.com
  • 基金资助:
    山东省医药卫生科技项目（202408020667）


Impact of C. leadbetteri on the proliferation,invasion and migration of Cal27 tongue squamous cell carcinoma cell line

  • Online:2025-10-25 Published:2025-12-03

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摘要： 目的 探讨从舌鳞状细胞癌(tongue squamous cell carcinoma,TSCC)患者组织中分离的二氧化碳嗜纤维菌（C. leadbetteri）对TSCC细胞系 Cal27增殖、侵袭及迁移的影响。方法 对从课题组前期收集的TSCC患者癌组织中分离得到的C. leadbetteri 菌株n95，通过复苏培养、菌落形态观察、革兰染色、16S rRNA序列分析及进化树构建进行鉴定。将C. leadbetteri n95菌液感染的Cal27细胞设为CAL27⁃C.L组，以未感染的细胞作为CAl27对照组，2 h后分别计数细胞和黏附细菌；采用CCK⁃8法检测不同感染复数(multiplicity of infection, MOI)（0、10、25）及不同时间点（0、24、48、72 h）下Cal27细胞的增殖能力，Transwell实验评估细胞迁移及侵袭能力。结果 经16S rRNA序列比对（与标准菌株H6253一致性达99.63%）及邻接法构建的系统进化树分析鉴定菌株n95为C. leadbetteri。细胞黏附实验结果显示，C. leadbetteri n95对Cal27细胞的黏附指数为1.62±0.57。CCK⁃8法结果显示，经MOI=10∶1的C. leadbetteri n95感染24 h后，Cal27细胞的增殖活性显著增强（P<0.05）。Transwell实验结果显示，CAL27⁃C. L组细胞侵袭及迁移能力均较CAL27组增强（均P<0.01）。结论 TSCC来源的C. leadbetteri n95菌株能有效黏附于Cal27细胞系，并在体外显著增强其增殖、迁移及侵袭能力。 

关键词: 舌鳞状细胞癌, C. leadbetteri, 增殖, 迁移, 侵袭

Abstract: Objective To investigate the impact of Capnocytophaga leadbetteri (C. leadbetteri ), isolated from tissues of patients with tongue squamous cell carcinoma (TSCC), on the proliferation, invasion, and migration of TSCC Cal27 cell line. Methods The C. leadbetteri strain n95, previously isolated and cultured by our research group from the tumor tissue of TSCC patients, were identified through revival culture, colony morphology observation, Gram staining, 16S rRNA sequencing analysis, and phylogenetic tree construction. Cal27 cells were infected with C. leadbetteri n95 suspension (CAL27⁃C. L group), while uninfected Cal27 cells (CAL27 group) served as the control. Adhesion counts of cells and bacterial were recorded 2 h post⁃infection. The proliferative capacity of Cal27 cells at different multiplicity of infection (MOI=0, 10, 25) and time points (0, 24, 48, 72 h) was assessed using the CCK⁃8 assay. Transwell assays were employed to evaluate the migration and invasion capabilities of cells. Results Based on 16S rRNA sequences alignment, which exhibits a 99.63% sequence identity with the reference strain H6253, and Neighbour⁃Joining phylogenetic tree, strain n95 was identified as C. leadbetteri. Cell adhesion assays demonstrated that the adhesion index of C. leadbetteri n95 to Cal27 cells was 1.62±0.57. The CCK⁃8 assay results demonstrated that the proliferation activity of Cal27 cells was significantly enhanced  (P<0.05) after 24 h of infection with C. leadbetteri n95 at an MOI of 10∶1 . Transwell assays revealed that the invasion and migration capabilities of the CAL27⁃C. L group were significantly enhanced compared to the CAL27 group (P<0.01). Conclusions C. leadbetteri n95, isolated from TSCC, effectively adheres to Cal27 cells and markedly boots their proliferation, migratory and invasive capacities under in vitro.
【Key words】 Tongue squamous cell carcinoma; C. leadbetteri; Proliferation; Migration; Invasion

Key words:  , Bladder cancer, Metformin, Metastasis, Adenosine monophosphate activated protein kinase, Kinesin family member 1B

中图分类号: 

  • R739.86