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中国癌症防治杂志

中国癌症防治杂志 ›› 2015, Vol. 7 ›› Issue (2): 90-94.doi: 10.3969/j.issn.1674-5671.2015.02.05

• 临床研究 • 上一篇    下一篇

穿刺植入细管注射阿霉素脂质体治疗大鼠脑胶质瘤的初步研究

  

  1. 福建医科大学孟超肝胆医院肝病研究所；福建省第二人民医院检验科；福州市菁华崇辉医院内科
  • 出版日期:2015-04-25 发布日期:2015-05-14
  • 通讯作者: 徐兵

Preliminary study of injecting adriamycin liposomes through a thin tube implanted in the brain for treating brain glioma in rats 

  • Online:2015-04-25 Published:2015-05-14
  • Contact: xubing1228@163.com

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摘要：

目的 建立一种经皮向脑间质内注射药物以抑制脑胶质瘤生长的方法。方法 通过埋于皮下的直通接头和带有冰套的细管经皮向SD大鼠脑内注射生理盐水 （带管正常对照组）；向脑胶质瘤模型大鼠脑内分别注射阿霉素 （ADR）脂质体 （ADR脂质体组）、ADR水剂 （ADR水剂组）、生理盐水 （盐水对照组）；各组大鼠在注射药物后5 d、10 d分别以同法再注射一次。在末次注射药物后4 d，从后3组大鼠中各取8只大鼠测定脑肿瘤体积和血清谷丙转氨酶 （ALT）、谷草转氨酶 （AST）、肌酐 （Cr）的水平。其余大鼠常规继续饲养，观察大鼠生存时间。结果 治疗14 d后，ADR脂质体组大鼠肿瘤体积为（27.5±6.4） mm3，明显低于盐水对照组的肿瘤体积（57.5±6.3） mm3（P<0.05）；ADR脂质体组大鼠肿瘤抑制率为（52.2±14.6）%，明显高于ADR水剂组的肿瘤抑制率（27.7±9.5）%（P<0.05）；ADR脂质体组大鼠平均生存期为（42.3±8.2） d，明显长于ADR水剂组的平均生存期（29.9±4.8） d （P<0.05）；ADR脂质体组和ADR水剂组大鼠的血清ALT、AST、Cr值与盐水对照组比较差异均不明显 （P均>0.05）。带管正常对照组大鼠全部存活50 d以上。 结论 带冰套和金属芯的细管易于穿刺植入脑内，通过皮下的直通接头和脑内的细管可经皮向脑内注入药物，并明显抑制脑肿瘤生长，延长荷瘤大鼠存活时间。

关键词: 脑肿瘤, 阿霉素, 脂质体, 接头, 经皮注射, 治疗

Abstract:

Objective To develop a percutaneous method of administering drugs into the brain in order to suppress glioma growth. Method Sprague-Dawley rats were injected in the brain with 0.9% NaCl solution through a direct adapter implanted under the skin and a thin tube jacketed with an ice sleeve，while rats with brain gliomas received 0.9% NaCl solution， aqueous adriamycin or ADR liposomes through a thin tube wrapped in an ice sleeve attached to a device implanted under the skin. The injections were repeated 5 and 10 d later. Starting at 4 d after the last injection，tumor volume and serum concen-trations of alanine aminotransferase（ALT），aspartate aminotransferase（AST），and creatinine（Cr）were measured in 8 animals from the saline control group，aqueous ADR group and ADR liposome group. The remaining animals in each group were implanted with tubes but not administered drug or saline in order to assess survival following surgery. Results By 14 d after the last injection，tumor volume was significantly smaller in the ADR liposome group （27.5±6.4） mm3 than in the saline control group（57.5±6.3） mm3（P<0.05）. Tumor growth inhibition was significantly greater in the ADR liposome group（52.2±14.6）% than in the aqueous ADR group（27.7±9.5）%（P<0.05），and mean survival was significantly longer in the ADR liposome group（42.3±8.2） d vs （29.9±4.8） d（P<0.05）. Serum concentrations of ALT，AST，Cr were similar between both ADR groups and the saline control group（P>0.05）. All rats in the control group survived more than 50 d. Conclusion It is possible to safely and effectively implant a percutaneous drug delivery system through a puncture in the brain. This system，comprising a thin polyester tube with an ice sleeve and metal core，was able to administer ADR to significantly suppress tumor grouth in rats without obvious side effects on liver or kidney function.

Key words: Brain neoplasm, Adriamycin, Liposome, Adapter, Percutaneous injection, Therapy