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中国癌症防治杂志

中国癌症防治杂志 ›› 2018, Vol. 10 ›› Issue (4): 283-285.doi: 10.3969/j.issn.1674-5671.2018.04.07

• 宫颈癌专栏 • 上一篇    下一篇

miR-502对宫颈癌Hela细胞增殖和转移的影响

  

  1. 新乡医学院第三附属医院妇产科
  • 出版日期:2018-08-25 发布日期:2018-09-25

Effect of miR-502 on proliferation and metastasis in cervical cancer cell Hela

  • Online:2018-08-25 Published:2018-09-25

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摘要：

目的 探讨miR-502对宫颈癌患者预后及其对宫颈癌Hela细胞增殖和转移的影响。方法 通过数据库分析miR-502表达与宫颈癌患者临床病理特征的关系及其对预后的影响。在Hela细胞中过表达miR-502-3p后,采用CCK-8检测细胞增殖情况,Transwell实验检测细胞迁移和侵袭情况。结果 数据库分析结果显示,高表达miR-502的宫颈癌患者总生存期更长,但与TNM分期无明显相关性。CCK-8检测结果显示,miR-502-3p类似物组细胞增殖率低于miR-502-3p抑制剂组[(78.82±1.41)% vs (124.06±7.72)%,P<0.05];Transwell 实验检测结果显示,miR-502-3p类似物组细胞迁移率较miR-502-3p抑制剂组低[(85.39±7.19)% vs (121.17±8.20)%,P<0.05],细胞侵袭率亦较低[(85.32±7.12)% vs (117.13±7.37)%,P<0.05]。结论 高表达miR-502的宫颈癌患者预后较好,可能与其抑制宫颈癌细胞增殖和转移有关。

关键词: 子宫肿瘤, miR-502, 预后, 增殖, 转移

Abstract:

Objective To investigate the effect of miR-502 on prognosis of patients with cervical cancer, and its effect on the prolif-eration and metastasis in cervical cancer cell Hela. Methods The relationship between miR-502 and clinicopathological characteristics of cervical cancer patients was analyzed. After overexpression of miR-502 in Hela cells, the proliferation of cells was measured by cell counting kit-8(CCK-8), while cell migration and invasion was detected using Transwell assay. Results Patients with high expression of miR-502 showed a better overall survival,but it did not correlated with tumor stage. Compared with the miR-502-3p suppressing group,the miR-502-3p analogues group significantly inhibited cell proliferation[(78.82±1.41)% vs (124.06±7.72)%, P<0.05], cell migration[(85.39±7.19)% vs (121.17±8.20)%, P<0.05], and cell invasion [(85.32±7.12)% vs (117.13±7.37)%, P<0.05]. Conclusions High expression of miR-502 can significantly  inhibit proliferation and metastasis by cervical cancer cells,and it may relate to better prognosis of cervical cancer patients.

Key words: Cervical neoplasms, miR-502, Prognosis, Proliferation, Metastasis