Abstract:  Objective To design a lentiviral vector-based system for exploring the effects of silencing the ribosomal protein S6 kinase 4（RSK4） gene in the human breast cancer cell line MCF-7. Methods A lentiviral RNA interference vector targeting the RSK4 gene was designed and stably transfected into MCF-7 cells.Expression of RSK4 mRNA and protein was analyzed by real-time PCR and Western blotting. Results Stable transfection led to 98.2% inhibition of RSK4 mRNA and 80.1% inhibition of RSK4 protein. Conclusion Stable transfection of the breast cancer cell line MCF-7 using a lentiviral RNA interference vector can significantly inhibit expression of RSK4 mRNA and protein.

Key words:  Breast neoplasms, RSK4, shRNA interference, Lentiviral