Abstract: Objective To investigate the effects of fresh Gecko active component on proliferation and apoptosis in human hepatocel-lular carcinoma cell line BEL-7404. Methods Solutions were prepared containing different concentrations of freeze-dried fresh Gecko active component powder， and these were added to BEL-7404 cultures.MTT assay was used to measure the antiproliferative effects of the different concentrations，and flow cytometry was used to assess the extent of apoptosis at the different concentrations. Results Fresh Gecko active component inhibited BEL-7404 proliferation relative to the control group（P<0.05） in a time- and dose-dependent manner.The component also induced apoptosis in BEL-7404 cells，with the highest concentration（2 560 μg/ml） showing statistically significant effects（P<0.05）. Conclusion Fresh Gecko active component can efficiently inhibit cell proliferation and induce apoptosis in the human hepatocellular carcinoma cell line BEL-7404.

Key words:  Liver neoplasms, Fresh Gecko active component, Hepatocellular carcinoma cell line BEL-7404, Proliferation, Apoptosis