Abstract: Osteosarcoma is a common primary malignant bone tumor characterized by high invasiveness and metastatic potential. Pulmonary metastasis is the primary cause of treatment failure and poor prognosis in patients. N6⁃methyladenosine (m6A) is the most abundant epigenetic modification in eukaryotic mRNA, has been confirmed to be deeply involved in the metastatic progression of osteosarcoma. This article systematically reviews the dynamic modification mechanism of m6A methylation and the functions of its core regulatory components. It elucidates the molecular mechanisms through which these regulatory factors,  by mediating RNA splicing, transport, translation, and degradation to regulate downstream target genes and related signaling pathways, ultimately driving osteosarcoma migration, invasion, and epithelial⁃mesenchymal transition. Additionally, explores the clinical potential of m6A modification as a diagnostic biomarker and therapeutic target for osteosarcoma metastasis, aiming to provide new directions for mechanistic research and clinical intervention of osteosarcoma metastasis.

Key words: Osteosarcoma, N6?methyladenosine, Tumor metastasis, Epigenetic regulation