Abstract: Objective To investigate the inhibitory effect of pAdBM5-mAFP-DC tumor vaccine on the transplanted liver cancer in mice.Methods 40 C57BL/6J mice were randomly divided into group A,B,C,D and E(8 mice in each group).Group A was immunized with mAFP gene-modified DC(pAdBM5-mAFP-DC);Group B was immunized with simple pAdBM5 plasmid; Group C was immunized with recombinant pAdBM5-mAFP plasmid;Group D was immunized with simple DC;Group E was injected with PBS.Each mouse in group A and D was immunized with 5×10~5 cells,in group B and C was immunized with 10.0μg plasmid DNA,in group E was injected with 0.1ml PBS.They were immunized once a week for a consecutive 4 weeks.At the third week,all the mice were transplanted with Hepa1-6 cells.All mice were killed 14 days after Hepa1-6 immunization.Results There was no tumorigenesis could be observed in 3 of 8 mice in group A.The average tumor weight of group A was far more lighter than that in the other groups,with the statistical significance(P<0.05 or 0.01).The tumor-inhibited rate of group A,B,C and D were 68.31%,13.23%,52.1%and 38.54%,respectively.The tumor-inhibited rate for group A was significantly higher than that in the other groups(P<0.05 or 0.01).Conclusion mAFP gene-modified DC tumor vaccine could induce immunoprotection and reduce the risk of tumorigenesis.

Key words: Dendritic cells, Transplanted liver cancer, DC tumor vaccine