Abstract: Objective SELDI technology was used to detect the changes of protein expression in cytokine-induced killer cells(CIK) which were culture in the medium contains placental factor(PF).Methods Peripheral blood mononuclear cells(PBMNCs) were collected from healthy human donors.After being induced as cytokine induced-killer(CIK),the cells were divided into two groups.The CIK group was cultured with 10% FBS culture media only,and the PF-CIK group was cultured with 10% FBS culture media that was added placental factor(PF).The proteomic spectra of CIK were obtained by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry(SELDI-TOF-MS) on WCX-2 chips.The differences of proteins between CIK group and PF-CIK group were compared.Results The results of SELDI showed that a total of 6 protein was significantly different(P<0.05),especially the four with molecular weight as 5636.077,6860.359,6072.814 and 5984.191.Conclusions PF might have the function that can change the expression level of CIK.SELDI technology can find out the differentially expressed protein in CIK effectively.

Key words: SELD, Placental factor, CIK