Abstract:

Objective To explore the expression of miR-204 in human breast cancer tissues and the MCF-7 cell line，and to investigate the influence of miR-204 on proliferation and apoptosis of breast cancer cells. Methods The miR-204 sequence was cloned based on information in the TCGA database. then it was used to generate recombinant virus and than transfected into MCF-7 cell，and the expressed microRNA was detected in infected cells using RT-qPCR. The proliferative ability of infected cells expressing miR-204 was tested using the MTT assay. Flow cytometry analysis was used to determine extent of apoptosis. Results  Expression of miR-204 was significantly lower in invasive breast cancer tissues than in normal breast tissue；expression correlated with lymph node and cancer metastasis. MTT assays showed that overexpression of miR-204 inhibited MCF-7 cell proliferation，and flow cytometry suggested that it significantly increased the rate of apoptosis. Conclusion miR-204 can inhib it breast cancer cell proliferation and induce apoptosis.

Key words: Breast neoplasm, miR-204, Breast cancer cell line MCF-7, Proliferation, Apoptosis