人鼻咽癌细胞CNE-2双向电泳样品制备方法的建立

Abstract

Abstract: Objective To establish and optimize the method of preparing protein sample for two-dimensional electrophoresis (2-DE) form human nasopharyngeal carcinoma cell line CNE-2.Method Three different Methods of extracting protein from CNE-2 cells were adopted.Then the protein samples were separated by 2-DE.Result The method of combining general lysate and Destreak reagent made the 2-DE maps with clearer image and higher resolution than the other two methods.ConclusionsA method of preparing sample from CNE-2 cells for 2-DE is established, which lays a foundation for further studies on proteome.

Key words: Two dimensional electrophoresis, Nasopharyngeal carcinoma, Proteome

HUANG Shi-ting,ZHU Xiao-dong1, QU Song1, et al.. Method of preparing sample for two dimensional electrophoresis from human nasopharyngeal carcinoma cell line CNE-2[J].Chinese Journal of Oncology Prevention and Treatment, 2010, 2(03): 169-171.

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Method of preparing sample for two dimensional electrophoresis from human nasopharyngeal carcinoma cell line CNE-2

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