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25 September 2010, Volume 2 Issue 03 Previous Issue    Next Issue

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Method of preparing sample for two dimensional electrophoresis from human nasopharyngeal carcinoma cell line CNE-2 HUANG Shi-ting,ZHU Xiao-dong1, QU Song1, et al. 2010, 2 (03):  169-171.  doi: Abstract ( 329 )   PDF (249KB) ( 1021 )   Save Objective To establish and optimize the method of preparing protein sample for two-dimensional electrophoresis (2-DE) form human nasopharyngeal carcinoma cell line CNE-2.Method Three different Methods of extracting protein from CNE-2 cells were adopted.Then the protein samples were separated by 2-DE.Result The method of combining general lysate and Destreak reagent made the 2-DE maps with clearer image and higher resolution than the other two methods.ConclusionsA method of preparing sample from CNE-2 cells for 2-DE is established, which lays a foundation for further studies on proteome. References | Related Articles | Metrics

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S100 A9 expression in hepatocellular carcinoma and its significance CHEN Bin,PENG Min-hao,LI Jia-quan,et al. 2010, 2 (03):  172-175.  doi: Abstract ( 387 )   PDF (331KB) ( 950 )   Save Objective To investigate the differential expression of S100 A9 protein in different phenotype of human hepatocellular carcinoma (HCC) tissues,and to explore the clinicopathologic significance.MethodsExpressions of S100 A9 protein in different liver tissue specimens were observed by immunohistochemistry assay. The specimens included 5 cases of normal liver,15 cases of cirrhosis caused by hepatitis B,and 48 cases of HCC that were with different phenotypes. The relativity between the results of immunohistochemistry assay and the clinicopathologic parameters were analyzed.Results S100 A9 protein localized in hepatocellular cytoplasm. Expression of S100 A9 protein in normal liver tissue and small HCC was negative. One-third cirrhosis caused by hepatitis B and 16 cases (33.3%) of HCC were S100 A9 positive. The positive expression rate of invasive HCC was higher than that of non-invasive HCC (52.17% vs.16%,P=0.019),while the positive expression rate of grade III HCC by Edmondson-Steiner classification was significantly higher than that of grade II (81.8% vs.18.9%,P=0.000).Clinicopathologic correlation study found that S100 A9 expression was closely related to HCC invasion and differentiation.ConclusionsExpression of S100 A9 protein in invasive HCC increases significantly.S100 A9 may be a potential and valuable molecular marker for HCC classification and invasion prediction. References | Related Articles | Metrics

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Effect of intraoperative implantation of slow-releasing 5-FU microparticles for preventing recurrence of hepatocellular carcinoma after curative resection ZHANG Zhi-Ming,ZHAO Yin-nong,LIU Jian-Yong,et al. 2010, 2 (03):  176-178.  Abstract ( 318 )   Save Objective To evaluate the efficacy of intraoperative implantation of slow-releasing 5-FU microparticles for preventing local recurrence of hepatocellular carcinoma (HCC) after curative resection.MethodsA total of 250 patients with HCC underwent curative resection from Jan 2007 to Jan 2010 was included in this study.The patients were randomly divided into 5-FU microparticles implantation group and control group.The 125 patients in implantation group were treated with hepatectomy plus implanting slow-releasing 5-FU microparticles on the wound surface,whereas the 125 patients in control group were treated with hepatectomy alone.All the patients were followed up regularly.ResultsThe recurrent rates of 1-,2-and 3-year after operation were 13.9%,31.9% and 58.8% respectively in implantation group,while they were 30.9%,40.9% and 70.9% respectively in control group.The differences between the two groups were statistically significant (P<0.05).ConclusionsIntraoperative implantation of slow-releasing 5-FU microparticles on the wound surface is an effective measure to prevent local recurrence of HCC after curative resection. References | Related Articles | Metrics

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Expressions of MMP-9 and TIMP-1 in non-small cell lung cancer and the relations to metastasis and prognosis CAO Ji-wei,LIU De-sen,PAN Hong,et al. 2010, 2 (03):  179-182.  Abstract ( 345 )   Save Objective To detect the expressions of MMP-9 and TIMP-1 in non-small cell lung cancer (NSCLC),and to explore their relations to metastasis and prognosis of NSCLC.MethodsA tissue chip was made by the samples from 113 cases of NSCLC and 20 cases of inflammatory pseudotumor.The expressions of MMP-9 and TIMP-1 of the tissue chip were detected by immunohistochemical staining.ResultsThe expressions of MMP-9 and TIMP-1 were not significantly related to patient’s age,sex and smoking situation,and tumor’s size,histological type and differentiation.However,the expressions of MMP-9 and TIMP-1 were closely correlated with lymph node metastasis,TNM and life span stages of the patients with NSCLC.ConclusionsThe expressions of MMP-9 and TIMP-1 could serve as parameters for predicting metastasis and prognosis of NSCLC. References | Related Articles | Metrics

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Method of four-dimension for prosthetic breast reconstruction LI De-quan,LIU Jian-lun,MO Qin-guo,et al. 2010, 2 (03):  183-186.  Abstract ( 320 )   Save Objective To search a new way of designing prosthesis for breast reconstruction.MethodsIn accordance with the contralateral uninjured breast,four dimensions including volume,transverse diameter,length wise and height were measured.The size of breast prosthesis was calculated according to the formula,and then the personalized breast prosthesis was selected from the inventory.Meanwhile,a volume of 20～25ml was added to or reduced from the breast prosthesis according to patient’s height and obesity.ResultThe four-dimension method for breast prosthetic reconstruction was adopted on 30 cases of breast cancer in postoperative phase I.The effect of the operation was satisfactory and the shape of the restructured breasts was fine.ConclusionsThe four-dimension method is exact,simple and useful for designing breast prosthesis. References | Related Articles | Metrics

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Expressions and significances of survivin and bcl-2 in cervical lesions LIANG Ying-ying, LI Qi, LI Jing 2010, 2 (03):  186-188.  Abstract ( 288 )   Save Objective To investigate the expressions and significances of survivin and bcl-2 in cervical carcinoma, CIN and normal cervical epithelial tissues.Methods Immunohistochemical staining was used to examine the expressions of survivin and bcl-2 in 50 cases of cervical carcinoma, 20 cases of CIN I,20 cases of CIN II~III and 20 cases of normal cervical epithelial tissues.Results The expressions of survivin and bcl-2 in carcinoma were stronger than that in CIN and normal cervical epithelial tissues (P<0.05), while there was no significant difference among different degrees of cervical carcinoma.Conclusion The expression of survivin and bcl-2 are closely related to carcinogenesis of cervix. References | Related Articles | Metrics

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Comparison of half-beam versus whole-beam irradiation on nasopharyngeal carcinoma ZHANG Wei-jian,CAI Chuan-shu,HONG Jin-sheng,et al. 2010, 2 (03):  188-191.  Abstract ( 343 )   Save Objective To explore whether the half-beam irradiation on nasopharyngeal carcinoma (NPC) could increase regional control rate and reduce radiation-induced lower cranial neuropathy (RILCN) compared to the effect of whole-beam irradiation.MethodsIn a non-random concurrent controlled trial,191 NPC patients who received definitive conventional radiation were divided into half-beam irradiation group (n=80) and whole-beam irradiation group (n=111).Kaplan-Meier method,log-rank test,Cox or Logistic regression model were used respectively to calculate the survival rate,compare the difference between two groups and carry through the multivariate analysis.ResultsThe 5-year overall survival rate (OS) of all patients was 67.3%,while it was 62.3% and 67.4% in half-beam group and whole-beam group respectively,and the difference between the two groups was not significant (χ2=0.244,P=0.621).Multivariate analysis showed patient’s gender and clinical stage were independent risk factors of OS.The 5-year regional control rate of half-beam group and whole-beam group were 80.6% and 83.1% respectively,and the difference between the two groups was not significant (χ2=1.425,P=0.237).Multivariate analysis showed N stage was independent risk factor for regional control rate.The incidence of RILCN of half-beam group and whole-beam group were 8.8% (7/80) and 9.0% (10/111) respectively,and the difference between the two groups was not significant (χ2=0.004,P=0.951).ConclusionsComparing to whole-beam irradiation,half-beam irradiation for NPC has no significant advantage on increasing regional control rate and reducing RILCN rate. References | Related Articles | Metrics

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Clinical analysis on 50 cases of hepatocellular carcinoma with bile duct tumor thrombus GUI Wen-bo,XIAO Kai-yin 2010, 2 (03):  192-195.  Abstract ( 373 )   Save Objective To investigate clinical feature and the effect of surgical treatment on primary hepatocellular carcinoma (HCC) with bile duct tumor thrombus (BDT).MethodsThe clinical feature and effect of surgical treatment on 50 cases of HCC with BDT were analyzed retrospectively.All the patients were treated in our hospital between 1998 and 2006,and were diagnosed through B ultrasound,CT,MRCP,AFP and exploratory celiotomy.Treatments included resection of HCC with BDT,cholangiotomy with BDT resection,and biliary tract prosthesis drainage.Results46 patients received radical operation,which including 26 cases treated by resection of HCC with BDT and 20 cases treated by hepatectomy with BDT clearance.4 cases were treated by biliary tract prosthesis drainage.For all of the patients,the postoperative complications ratio was 26.0% (13/50),and the perioperative death ratio was 12.0% (6/50).The 1-,2-and 3-year actuarial survival were 59.1% (26/44),29.5% (13/44) and 20.5% (9/44) respectively.The average survival time was 19.6 months,with the longest as 121 months.The univariate analysis showed that the factors such as AFP level,tumor size,operation mode and thrombi site could affect survival time significantly.The multivariate analysis showed that the independent predictor for survival time was surgical operation mode.ConclusionsSurgical operation mode is the most important prognostic factor for HCC patient with BDT.Surgical removal of primary tumor and BDT is crucial for prolong patient’s survival time. References | Related Articles | Metrics

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The effect of docetaxel and fluorouracil combined with oxaliplatin or cisplatin on advanced nasopharyngeal carcinoma MO Han-wen,LI Li-ping,Huang Li-hua,et al. 2010, 2 (03):  196-198.  Abstract ( 438 )   Save Objective To evaluate the efficacy and safety of the 5-day chemotherapy by the combination of docetaxel,oxaliplatin and fluorouracil (DOF),or docetaxel,cisplatin and fluorouracil (DCF) as a first-line treatment for patients with advanced nasopharyngeal carcinoma.Methods60 patients with advanced nasopharyngeal carcinoma were randomly divided into group A and group B.Patients in group A (30 cases) were treated with DOF proposal as using docetaxel 75mg/m2 in d1,oxaliplatin 135mg/m2 in d1 and fluorouracil 500mg/m2 in d1～d5.Patients in group B (30 cases) were treated with DCF proposal as using docetaxel 75mg/m2 in d1,cisplatin 20mg/m2 in d1～d4 and fluorouracil 500mg/m2 in d1～d5.Both regimens were repeated once every 3 weeks for at least 2 cycles.ResultsThe clinical response rate,completed response rate,partial response rate,median progression time,median survival time,1-and 2-year survival rate were 86.67%,6.67%,60.00%,5.9mo,10.6mo,56.70% and 15.35% in group A respective1y,and 83.33%,3.33%,53.33%,5.9mo,11.4mo,58.30% and 12.47% in group B respectively.The main toxicities in two groups were bone marrow depression,nausea and hair loss,but all of them were mild and tolerable.ConclusionsThe combined chemotherapy of docetaxel,oxa1iplatin and fluorouracil,as well as docetaxel,cisplatin and fluorouracil are effective and tolerab1e for treating patients with advanced nasopharyngeal carcinoma.Comparing with best sustaining treatment,combined chemotherapy can prolong patient’s survival time and improve their life quality.In particular,the former regimen has less influence on life quality than the latter. References | Related Articles | Metrics

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Clinical analysis of electronic bronchoscope on 320 cases of elderly patient with lung cancer WANG Peng 2010, 2 (03):  199-202.  Abstract ( 317 )   Save Objective To analyze the feature of electronic bronchoscope on elderly patient with lung cancer, and to investigate the correlation among the histopathologic type of lung cancer, gender and the feature under bronchoscope.Methods320 patients with lung cancer older than 65 years were observed with electronic bronchoscope.ResultsAmong the 320 patients, cases of the central bronchogenic and the peripheral were 199 (62.2%) and 121 (37.8%) respectively. The most common histopathologic type was squamous cell cancer which had totally 176 (55.0%) cases. The percentage of adenocarcinoma and small cell lung cancer was 25.0% and 16.6% respectively. Under electronic bronchoscope, the cases of proliferation type were 162 (50.1%), mixed type 85 (26.6%), infiltration type 66 (20.1%) and outside oppression type 7 (2.2%). Among the patients with squamous cell cancer, male was significantly more than female (P=0.0000). While among the adenocarcinoma and small cell lung cancer, female was significantly more than male (P=0.0476 and P=0.0245 respectively). The feature under the electronic bronchoscope was significantly related to histopathologic type (P=0.0000). Among the cases of squamous cell cancer and small cell lung cancer, the cases of central bronchogenic cancer were significantly more than that of peripheral cancer (P=0.0476 and P=0.0245 respectively). Among the cases with adenocarcinoma, the cases of peripheral cancer were significantly more than that of central bronchogenic cancer (P=0.0000).ConclusionsCentral bronchogenic cancer, squamous cell cancer and adenocarcinoma are the common types among the elderly patients with lung cancer, and proliferation is the most common feature under bronchoscope. Electronic bronchoscope play an important role in diagnosis of lung cancer, for it is simple to operate and is well to be tolerated. References | Related Articles | Metrics

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Effect of concurrent chemoradiotherapy on intermediate and advanced cervical cancer DONG Chun-li,KONG Yan,DING Yan,et al. 2010, 2 (03):  202-205.  Abstract ( 351 )   Save Objective To investigate the curative effect and adverse reaction of concurrent chemoradiotherapy (CCRT) on intermediate and advanced cervical cancer.Methods136 patients with intermediate or advanced cervical cancer were randomly divided into CCRT group and radiotherapy group,each group had 68 patients.Radiotherapy for both groups was by using high dosage rate of 192Ir on intracavity and external portion.Chemotherapy for the patients in CCRT group was by using three cycles of Cisplatin plus 5-FU.ResultsTotal effective rates in CCRT group and radiotherapy group were 95.6% and 83.8% respectively (χ2=5.096,P=0.024).The 1-,3-and 5-year survival rates in CCRT group were 88.2%,75.0% and 66.2% respectively,while they were 80.9%,60.3% and 50.0% respectively in radiotherapy group.The incidence rates of early radioactive rectum reaction in CCRT group and radiotherapy group were 17.6% and 13.2% respectively,and the incidence rates of bladder reaction in these two groups were 7.4% and 4.4% respectively.The metastasis rate in CCRT group was much lower than that in radiotherapy group.The bone marrow suppression and gastrointestinal reaction were more serious in CCRT group than those in radiotherapy group,but patients were usually able to tolerate the sickness after symptomatic treatment.ConclusionsThe effect of CCRT on intermediate and advanced cervical cancer is superior to radiotherapy,as it could reduce metastasis rate and enhance survival rate. References | Related Articles | Metrics

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To evaluate the clinical value of ~(99m)Tc-DX integrated scintigraphy on 167 patients with breast cancer sentinel lymph node YAO Zhong-qiang, XIAO Guo-you, LI Dang-sheng, et al. 2010, 2 (03):  206-208.  Abstract ( 324 )   Save Objective To evaluate the clinical value of 99mTc-DX combined with methylene blue staining and γ-detecting probe on biopsy or cleaning surgery of sentinel lymph nodes in patients with breast cancer.Methods167 patients with breast cancer were injected 99mTc-DX 37-74 MBq (1～2mCi) 14～16 hours before surgery to localize the sentinel lymph nodes. According to the scintigraphy of 99mTc-DX, the sentinel lymph nodes were detected by methylence blue staining and γ-detecting probe during biopsy or cleaning surgery. The ratio of correctly localized lymph node for biopsy or cleaning surgery was analyzed according to the result of histopathologic examination.Results The ration of correctly localized sentinel lymph node by 99mTc-DX was 87.4%, while by 99mTc-DX combined with methylene blue staining and γ-detecting probe was 91.6%. The ration of false negative in the combined group was 4.6%.Conclusion 99mTc-DX scintigraphy can show exactly the location, quantity and drainage situation of sentinel lymph nodes in patient with breast cancer. It can help to guide γ-detecting probe during biopsy or cleaning surgery on sentinel lymph nodes of the patient with breast cancer. References | Related Articles | Metrics