Abstract: Objective To study the association between polymorphism of ESR1 gene in primary liver cancer(PLC) in Fusui area of Guangxi.Methods 50 cases of PLC with Zhuang nationality,50 cases of PLC with Han nationality,37 cases from high risk population with Zhuang nationality,37 cases from high risk population with Han nationality,30 cases from normal population with Zhuang nationality and 30 cases from normal population with Han nationality were enrolled in this study.SNP locus rs 9340772 of ESR1 gene was selected as genetic marker.The genotypes and genetic mutation frequency of all the cases were detected by polymerase chain reaction combined with restriction fragment polymorphism method(PCR-RFLP).Results There was no mutation in SNP locus rs 9340772 of ESR1 in PLC group,the high risk population group and the normal population group. The expression of ESR1 showed no statistically significant difference among the three groups(P>0.05).Conclusion Polymorphism SNP locus rs 9340772 of ESR1 gene is not associated with PLC.

Key words: Primary liver cancer, Estrogen receptor, Gene polymorphism