Objective To investigate the role of Interleukin-17（IL-17） single-nucleotide polymorphisms（SNPs） rs2275913 and rs763780 in the development of gastric cancer. Methods A total of 355 Han Chinese patients with gastric cancer and 300 healthy Han Chinese controls were enrolled from the Qingdao area. Genomic DNA was extracted from peripheral blood，and rs2275913 and rs763780 genotypes were determined using PCR-based DNA sequence analysis and direct DNA sequencing. Relationships between the sequences and risk of gastric cancer were explored. Results Frequencies at IL-17 rs2275913 differed significantly between the cancer group and healthy group （χ2=17.192，P<0.001）. Subjects with the AA genotype were obviously more susceptible to gastric cancer than those with the GG genotype （χ2=16.829，P<0.05；OR=2.891，95%CI=1.721-4.857）. The GA genotype was associated with an increased risk of gastric cancer relative to the GG genotype，but the trend did not achieve significance（χ2=0.878，P>0.05）. Frequencies at IL-17 rs763780 were similar between the cancer group and healthy group（χ2=1.381，P>0.05）. Conclusions The A allele of IL-17 rs2275913 is associated with increased susceptibility to gastric cancer in Han Chinese from Qingdao. IL-17 rs763780 SNPs show no association with risk of gastric cancer.