Abstract: Objective To establish a stable green fluorescent protein（GFP）transgenic mouse model of hepatocellular carcinoma （HCC）and observe changes in fluorescence during carcinogenesis. Methods GFP transgenic mice were divided into an experimental group（n = 50）and a control group（n = 10）.HCC was induced in the experimental group by treatment with diethylnitrosamine（DENA）/carbon tetrachloride（CCl₄）/ethanol for 20 weeks. Animal body weight was monitored weekly.At weeks 4，12，and 16，some animals were sacrificed and frozen sections of liver tissue were prepared for fluorescence analysis，and HE-stained paraffin sections were pre-pared for observing dynamic histopathologic changes.At week 20，the remaining mice were sacrificed，and the liver tumors were used to establish primary cultures，in which the expression and distribution of fluorescence was studied. Results In the experimental group，30% of mice（15/50）died，while no death occurred in the control group.Hepatitis， cirrhosis，precancerous lesions and HCC appeared，respectively，at weeks 4，12，16 and 20.GFP protein expression was detected by fluorescence microscopy in frozen sections collected at weeks 4，12，and 16，as well as in the cytoplasm and nuclei of cultured tumor cells at week 20. Conclusion GFP expres-sion can be dynamically observed in cancer cells in this GFP transgenic mouse model of HCC.

Key words: Transgenic GFP mice, Hepatocellular carcinoma model, GFP protein expression