Table of Content

25 June 2012, Volume 4 Issue 2 Previous Issue    Next Issue

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Key technical issues on resection of large hepatocellular carcinoma YANG Lian-Yue 2012, 4 (2):  97-100.  doi: 10.3969/j.issn.1674-5671.2012.02.01 Abstract ( 407 )   PDF (372KB) ( 683 )   Save Related Articles | Metrics

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Treatment of percutaneous radiofrequency ablation on hepatocellular carcinoma CHEN Min-Shan 2012, 4 (2):  101-105.  doi: 10.3969/j.issn.1674-5671.2012.02.02 Abstract ( 445 )   PDF (422KB) ( 528 )   Save Related Articles | Metrics

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treatment of percutaneous radiofrequency ablation on hepatocellular carcinoma LIU Guang-Jian, LV Ming-De 2012, 4 (2):  106-109.  doi: 10.3969/j.issn.1674-5671.2012.02.03 Abstract ( 495 )   PDF (422KB) ( 521 )   Save Related Articles | Metrics

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PTEN genetic mutations and mRNA expression in cases of human hepatocellular carcino-ma exposed to hepatatitis B virus and aflatoxin B1 in Guangxi ZHU Hai, QI Lu-Nan, MIAO Zhi-Guo, PENG Tao, LI Le-Qun, MA Liang, YOU Xue-Mei 2012, 4 (2):  110-114.  doi: 10.3969/j.issn.1674-5671.2012.02.04 Abstract ( 414 )   PDF (436KB) ( 450 )   Save Objective To explore gene mutations and mRNA expression of PTEN in cases of hepatocellular carcinoma （HCC） exvposed to both hepatitis B virus and aflatoxin B1 （HBV/AFB1）. Methods A total of 108 cases of HCC from different districts of Guangxi were divided into 4 groups according to their exposure to HBV and AFB1：group A，HBV（+）/AFB1-DNA（+），48 cases；group B，HBV （+）/AFB1-DNA（-），27 cases；group C，HBV（-）/AFB1-DNA（+），19 cases；and group D，HBV（-）/AFB1-DNA（-），14 cases.PTEN mutations in exons 4，5，and 8 were detected by PCR and direct sequencing，and mRNA expression was analyzed by RT-PCR. Results PTEN mutations in exons 4，5，or 8 were not found in any of the cases. However，a large deletion at the junction of exon 4 and intron 4 was found in 61 cases，corresponding to a deletion rate of 56.4%.The rate of PTEN deletion was 60.4% in group A, 62.9% in group B，47.3% in group C and 46.6% in group D.The deletion rate was not statistically significant among the four sub-groups.Semi-quantitative gray values of the PTEN mRNA expression assay were as follows： group A，0.54 ± 0.13；group B，0.59± 0.16；group C，0.97 ± 0.16；group D，0.92 ± 0.13. The pair of groups A/B and the pair C/D differed significantly from each another in PTEN mRNA expression（PAC=0.002，PAD=0.032，PBC=0.000，PBD=0.011）. Conclusions Down-regulation of PTEN mRNA expression is a frequent molecular event in HCC involving double exposure to HBV/AFB1 in Guangxi，and it may be related to HBV infection.AFB1 and HBV may act synergistically to down-regulate PTEN mRNA levels. Related Articles | Metrics

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A green fluorescent protein transgenic mouse model of hepatocellular carcinoma SUN Zhong-Liang, LUO Dian-Zhong, KUANG Xiao-Cong, DANG Yi-Wu, CAI Jie 2012, 4 (2):  114-119.  doi: 10.3969/j.issn.1674-5671.2012.02.05 Abstract ( 575 )   PDF (634KB) ( 541 )   Save Objective To establish a stable green fluorescent protein（GFP）transgenic mouse model of hepatocellular carcinoma （HCC）and observe changes in fluorescence during carcinogenesis. Methods GFP transgenic mice were divided into an experimental group（n = 50）and a control group（n = 10）.HCC was induced in the experimental group by treatment with diethylnitrosamine（DENA）/carbon tetrachloride（CCl4）/ethanol for 20 weeks. Animal body weight was monitored weekly.At weeks 4，12，and 16，some animals were sacrificed and frozen sections of liver tissue were prepared for fluorescence analysis，and HE-stained paraffin sections were pre-pared for observing dynamic histopathologic changes.At week 20，the remaining mice were sacrificed，and the liver tumors were used to establish primary cultures，in which the expression and distribution of fluorescence was studied. Results In the experimental group，30% of mice（15/50）died，while no death occurred in the control group.Hepatitis， cirrhosis，precancerous lesions and HCC appeared，respectively，at weeks 4，12，16 and 20.GFP protein expression was detected by fluorescence microscopy in frozen sections collected at weeks 4，12，and 16，as well as in the cytoplasm and nuclei of cultured tumor cells at week 20. Conclusion GFP expres-sion can be dynamically observed in cancer cells in this GFP transgenic mouse model of HCC. Related Articles | Metrics

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Expression and mutation analysis of β-catenin in patients with hepatocellular carcinoma in Guangxi MIAO Zhi-Guo, QI Lu-Nan, ZHU Hai, PENG Tao, LI Le-Qun 2012, 4 (2):  119-122.  doi: 10.3969/j.issn.1674-5671.2012.02.06 Abstract ( 536 )   PDF (404KB) ( 430 )   Save Objective To explore β-catenin gene mutations and protein expression in patients with hepatocellular carcinoma（HCC） in Guangxi. Methods β-catenin gene mutations and protein expression were analyzed in 108 HCC tissues by direct sequencing and immunohistochemistry，respectively. Results Mutations in the β-catenin gene were found in 12 of 108 HCC tissues， giving a mutation rate of 11.1%.The overall rate of positive β-catenin protein expression was 70.0%，including samples that were nuclear-positive （14.6%），cytoplasm-positive（57.3%）and membrane-positive（28.0%）.The protein was expressed in 100% of the tissues carrying β-catenin gene mutations. Conclusions HCC patients in Guangxi show a relatively low rate of β-catenin gene mutation.These mutations may play an import role in hepatocarcinogenesis by causing over-expression of β-catenin protein. Related Articles | Metrics

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Establishment of a drug-resistant liver cancer cell line and its cancer stem cell characteris-tics TAO Lu, CAI Jie, KUANG Xiao-Cong, CHEN Xiang-Yi 2012, 4 (2):  123-126.  doi: 10.3969/j.issn.1674-5671.2012.02.07 Abstract ( 632 )   PDF (345KB) ( 723 )   Save Objective To establish a drug-resistant liver cancer cell line and examine its cancer stem cell characteristics. Methods The drug-resistant cell line BEL-7404 was induced using a concentration gradient of adriamycin（ADM）.Basic characteristics of the drug-resistant cell line were analyzed and compared to those of the parental cell line，such as cell growth，cell doubling time，cell cycle，resistance index（RI），single cell culture，colony formation and balloon cell culture. Results Similar to its parental cell line，the drug-resistant BEL-7404 line showed an epithelioid growth pattern，but with slightly more spindle cells.BEL-7404 cells had an RI of 292，and their cell proliferation rate was significantly slower than that of the parental cells，with increased cell doubling time and sig-nificantly lower rates of colony formation and balloon cell formation. Conclusions Sustained incremental exposure to low concentrations of ADM can induce a drug-resistant cell line，though the cancer stem cell characteristics of this line are not obvious. Related Articles | Metrics

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Dynamic changes in liver cancer stem cell marker CD90 during chemically induced hepa-tocarcinogenesis ZHANG Zhen-Ni, KUANG Zhi-Peng, YANG Fan, WU Ji-Ning 2012, 4 (2):  127-130.  doi: 10.3969/j.issn.1674-5671.2012.02.08 Abstract ( 559 )   PDF (494KB) ( 479 )   Save Objective To explore the expression of the CD90 gene at different stages of chemically induced hepatocellular carcino-ma（HCC）in C57BL/6J mice. Methods HCC was chemically induced in an experimental group of 50 male mice，while a control group of 50 male mice was raised normally.Every 4 weeks，some animals were sacrificed to collect specimens.Changes in CD90 expression were detected by RT-PCR，FQ-RT-PCR and Western blot，while pathological changes were assessed using HE staining. Results After 16 weeks of chemical induction，the experimental mice had obvious nodules with diameters of 2~25 mm. Tissue in the experimental group showed pathological changes consistent with well-differentiated HCC，including structural disorder，dysplasia，pathological mito-sis and partial cirrhosis.RT-PCR，FQ-RT-PCR and Western blots showed CD90 expression to be significantly higher in the liver tissue of the experimental mice than in the liver tissue of control mice after 16 weeks of induction（P<0.05）. Conclusion The expression of liver cancer stem cell marker CD90 increases gradually during hepatocarcinogenesis.CD90 plays a regulatory role in the occurrence and development of HCC. Related Articles | Metrics

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Functional comparison of induced dendritic cells from peripheral blood of patients with hepatocellular carcinoma and healthy individuals LUO Xiao-Ling, XIE Yu-An, LIANG An-Min, KUANG Zhi-Peng, WU Ji-Ning 2012, 4 (2):  131-135.  doi: 10.3969/j.issn.1674-5671.2012.02.09 Abstract ( 477 )   PDF (642KB) ( 445 )   Save Objective To investigate the potent anti-tumor activity of induced dendritic cells（DC）derived from the peripheral blood of patients with hepatocellular carcinoma（HCC），and to lay the foundation for a DC vaccine against HCC. Methods DC were isolated from the peripheral blood of HCC patients and healthy individuals and induced in vitro using rhGM-CSF，rhIL-4 and HCC antigen. The morphology of DC was observed by phase contrast microscopy， and surface molecule expression was analyzed by fluorescence activated cell sorting.IL-12 released by DC into the culture medium was detected by ELISA.T cell proliferation was quantitated by 3H-TdR uptake，and cytotoxic of T-lymphocyte（CTL）activity was assessed using the LDH release assay. Results Comparison of induced DC from HCC patients and induced DC from healthy individuals revealed no significant differences in growth characteristics；expression levels of surface molecules such as HLA-DR，CD1a，CD83，CD80，CD86，ICAM-1，and IL-12；and ability to stimulate T lymphocytes and induce CTL activity（P>0.05）. Conclusions Induced DC from HCC patients can exert anti-tumor effects similar to those observed with induced DC from healthy individuals.These results provide an experimental basis for developing HCC immunotherapies involving DC. Related Articles | Metrics

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Expression of CCT subunit γ in aflatoxin B1-induced hepatocarcinogenesis in Wistar rats OU Chao , YANG Chun, SU Jian-Jia, LI Yuan, ZHENG Hai-Ping, Tang-Yan-Ping, LI Huang-Yi, CAO Ji 2012, 4 (2):  136-139.  doi: 10.3969/j.issn.1674-5671.2012.02.10 Abstract ( 447 )   PDF (355KB) ( 404 )   Save Objective To investigate the protein expression and possible role of CCT subunit γ in hepatocarcinogenesis induced by aflatoxin B1（AFB1）in rats. Methods A total of 45 Wistar rats were randomly divided into a control group and an AFB1 group，which was injected intraperitoneally with 200 μg/kg AFB1.Liver biopsies were performed on all animals at weeks 13，33 and 53.Animals were sacrificed at week 73 and liver tissues were collected.Protein expression of CCT subunit γ was dynamically examined by immunohistochemistry during different phases of AFB1-induced hepatocarcinogenesis. Results The protein expression levels of the CCT subunit γ were significantly higher in HCC tissues of the AFB1 group（100%，10/10）than in tissues of the control group （30%，3/10）. The protein level gradually increased during development of hepatic carcinoma， with the level at week 73 significantly higher than at weeks 13 and 33（P<0.01）.At week 73，the level of CCT subunit γ was higher in the AFB1 group than in the control group （P<0.05）.The expression level of CCT subunit γ did not differ significantly between the two groups at other times（P>0.05）. Conclusion Overexpression of CCT subunit γ may be related to HCC carcinogenesis. Related Articles | Metrics

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Study on gene polymorphisms of glutathione S-transferase GSTM1 and GSTT1 in high-risk families for liver cancer in Fusui county，Guangxi CHEN Yuan-Yuan, DING Fei, XIE Yu-An 2012, 4 (2):  140-144.  doi: 10.3969/j.issn.1674-5671.2012.02.11 Abstract ( 489 )   PDF (307KB) ( 380 )   Save Objective To study the relationship between family clustering of hepatocellular carcinoma（HCC）and glutathione S-transferase M1 and T1 polymorphisms，to explore gene-gene interactions in HCC，and to assess differences in HCC risk between probands and first-degree relatives. Methods Peripheral blood samples were collected from 76 members of 21 HCC-clustering families and 68 members of 21 control families in the Zhuang population of Fusui county，Guangxi province.This area has a high incidence of HCC.M1 and T1 genotypes were determined by multiplex polymerase chain reaction，and serum HBsAg was measured by ELISA. Genotypes and clinical data were analyzed statistically to search for associations. Results The frequency of the GSTM1 null genotype was 67.1% in the family constellation of the HCC group and 36.8% in controls（P = 0.000）.The frequency of the GSTT1 null genotype was 40.8% in the family constellation of the HCC group and 13.2 % in controls（P = 0.005），while the prevalence of the combined GSTM1 and GSTT1 null genotype was 31.6% and 2.9%（P=0.000）.Frequencies of the GSTM1 or GSTT1 null genotype and of the com-bined GSTM1 and GSTT1 null genotype did not differ significantly between probands and first-degree relatives. Conclusions ①A re-lationship was found between familial clustering of HCC and frequencies of the GSTM1 and GSTT1 polymorphisms.②A combined null genotype at GSTM1 and GSTT1 positively correlated with liver cancer occurrence，and it interacted synergistically with hepatocar-cino-genesis.③The risk of HCC between first-degree relatives and probands is not significantly different. Related Articles | Metrics

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The effect of brachytherapy involving 125I seeds and 5-FU on growth and pathological changes of H22 cells HUANG Bai-Sha, YANG Wei-Ping, WEI Chang-Yuan, QIN Qing-Hong, LI Ting 2012, 4 (2):  145-149.  doi: 10.3969/j.issn.1674-5671.2012.02.12 Abstract ( 452 )   PDF (470KB) ( 429 )   Save Objective To investigate the effects of a combination of interstitial implantation of an 125I seed with 5-FU on growth and pathological changes of hepatocarcinoma cell line H22. Methods A total of 40 KM mice were transplanted subcutaneously with H22 cells.Tumor-bearing mice were then divided randomly into 4 groups（each n = 10）.An 125I seed and 5-FU were embedded into the tumor of each mouse in group A；mice in groups B and C were treated，respectively， with an 125I seed or 5-FU；mice in group D did not receive any treatment.All animals were sacrificed 28 days later；survival rate and tumor size were measured，and tissue samples were analyzed by routine histopathology and electron microscopy. Results Mean tumor volumes in each group were as follows：group A，1.38±0.11 cm3；group B，2.11±0.19 cm3；group C，2.79±0.23 cm3；group D，4.34±0.29 cm3.Volumes were significantly different a-mong the groups （P<0.01）. The extent of tumor volume inhibition was 68.21% in group A， 51.38% in group B and 35.71% in group C. Histopathological observation showed that the major change in group A was cell necrosis， while samples from groups B and C showed scattered necrotic cancer cells，with a large number of lymphocytes around. Electron microscopy of group A samples showed complete or incomplete necrosis and sometimes apoptosis of cancer cells，while samples from groups B and C showed most tumor cells to have an integrated nuclear membrane，dense chromatin and numerous cytoplasmic protuberances or chromatolysis and apoptotic bodies.Samples from these two groups also showed numerous aggregated apoptotic cancer cells. Conclusion 125I seed interstitial im-plantation can promote apoptosis in H22 cells.Use of an 125I seed combined with 5-FU interstitial chemotherapy can further promote tumor cell degeneration， necrosis and apoptosis.The combination of 125I and 5-FU has synergistic effects，providing a new option for treating liver cancer. Related Articles | Metrics

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Correlation between TNF-α polymorphisms and family clustering of primary liver cancer in Guangxi WANG Chong-Ke, ZHANG Chun-Yan, HUANG Tian-Ren, YU Jia-Hua, DENG Wei, HUANG Qiong-Hua, DONG Xin, HU Hong-Bo 2012, 4 (2):  149-153.  doi: 10.3969/j.issn.1674-5671.2012.02.13 Abstract ( 575 )   PDF (417KB) ( 532 )   Save Objective To explore the correlation between genetic polymorphisms of tumor necrosis factor （TNF-α-308，TNF-α-238） and family clustering of primary liver cancer（PLC） in Guangxi. Methods Blood samples were collected from 124 families with a high incidence of PLC and from 129 control families.Genotype and alleles of TNF-α-308 and TNF-α-238 were determined by SNP microar-ray technology. Results The frequencies of TNF-α-308G/A genotype and TNF-α-308A allele in the PLC-clustering families （22.6%and 11.3%）were significantly higher than those in the control families（11.6% and 5.8%）.However，the two groups showed no statisti-cally significant differences in frequencies of the TNF-α-238 genotype or allele（P>0.05）. Conclusions TNF-α-308 genetic polymorph-ism is associated with susceptibility in families with high PLC incidence in Guangxi.In particular，the TNF-α-308A allele is proba-bly a risk factor for PLC in these families. In contrast，the TNF-α-238 polymorphism may not be associated with family clus-tering of PLC in Guangxi. Related Articles | Metrics

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Ability of recombinant human endostatin to reduce H22 ascites in mice Cao-Qian-Qian, XIE Wei-Min, TANG Jing, WU Jie, LU Yong-Kui, ZHOU Wen-Xian, WANG Hong-Xue, YI Wei-Li, ZHENG Ze-Bin 2012, 4 (2):  153-157.  doi: 10.3969/j.issn.1674-5671.2012.02.14 Abstract ( 562 )   PDF (353KB) ( 768 )   Save Objective To investigate the effect of intraperitoneal endostar administration to treat H22 ascites in mice. Methods A mouse model of ascites was established by inoculating 110 Kunming mice with H22 cells（2×106，i.p. injection）.Mice were then di-vided randomly into 5 groups:control （normal saline）；low endostar dose（4 mg/kg•day），intermediate endostar dose（8 mg/kg•day）， high endostar dose（12 mg/kg•day）and positive control（DDP 0.6 mg/kg•day）.After an adjustment period of 24h，the groups were treated from day 1 to day 10 by i.p. injection as indicated above and sacrificed 24h after drug withdrawal.Data were collected on vol-ume of ascitic fluid，metastases of abdominal organs and lungs and survival time.Peritoneal membrane permeability was assessed us-ing Evan blue staining. Results Intermediate and high doses of endostar significantly inhibited the production of ascites in H22 as-citesbearing mice，reduced the frequency of metastases in the abdominal viscera and lungs，and lengthened average survival time. Conclusion Intraperitoneal administration of endostar can reduce ascites in H22 ascites-bearing mice. Related Articles | Metrics

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Whole-body γ-knife radiotherapy combined with Chinese herbal drugs to treat large pri-mary liver cancer PANG Jun, CHEN Hao-Tao, CHEN Yan, HAO Guang-Yuan, ZHANG Ming-Xing, FAN Rong, YANG Xin-Zhong, LI Chang-Qing 2012, 4 (2):  158-162.  doi: 10.3969/j.issn.1674-5671.2012.02.15 Abstract ( 495 )   PDF (350KB) ( 526 )   Save Objective To investigate the clinical application and short-term effects of stereotactic whole-body γ-knife radiotherapy combined with Chinese herbal drugs to treat patients with large primary liver cancer. Methods A total of 100 patients with large pri-mary liver cancer hospitalized from July 2009 to February 2011 were randomly divided into a treatment group and control group.The treatment group was treated with whole-body γ-knife radiotherapy combined with Chinese herbal drugs.Stereotactic super-γ-knife ra-diotherapy was carried out 4~12 times，5 times per week（tumor size，55.9~2579.0 cm3；isodose curve，50％~60%；total radiation dose， 33~40 Gy；fractional dose，3.3~5.0 Gy）.Treatment with Chinese herbal drugs（prescription No. 1）was carried out during radiotherapy or for 2~3 months after the radiotherapy.Prescription No.2 was used for 2~3 months after radiotherapy.The control group was treated with only stereotactic super-γ-knife radiotherapy.Therapeutic effects and side effects of the two treatments were analyzed. Results The overall response rates at 3 months after treatment were 95.83% in the treatment group and 94.23% in the control group.The treatment group showed 6-month and 1-year survival rates of 75.00% and 71.15%，compared to 60.42% and 57.69% for the control group.The two groups did not differ significantly in curative effects（P>0.05），whereas they did differ significantly in adverse reactions（P<0.05）. Conclusion Stereotactic super-γ-knife radiotherapy is effective for treating large primary liver cancer， and the combination of whole-body γ-knife with Chinese herbal drugs can improve quality of life significantly and reduce the adverse reactions due to radiotherapy. Related Articles | Metrics

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Prognostic factors when using three-dimensional conformal radiotherapy to treat primary liver cancer: an analysis of 283 cases HUANG Shang-Xiao, LIU Min, LIANG Shi-Xiong, WU Chun-Hua, ZHU Xiao-Dong, HUANG Qi-Fang, CHEN Long 2012, 4 (2):  163-166.  doi: 10.3969/j.issn.1674-5671.2012.02.16 Abstract ( 430 )   PDF (276KB) ( 403 )   Save Objective To evaluate the efficacy of three-dimensional conformal radiotherapy（3DCRT）to treat primary liver cancer （PLC）and investigate the prognostic factors associated with its use. Methods PLC patients（n = 283）were treated with 3DCRT using a total dose of 51.85±6.55 Gy（4.55±1.02 Gy/fraction，3 fractions/week，10±4 total fractions）. Results The response rate（CR+PR）was 47.35%（134/283）.The 1-，3-，and 5-year overall survival rates were 57.0%，29.0% and 18.0%，respectively.Gender，CLIP stage，GTV，PVTT and Child-Pugh class were found to significantly affect overall survival（P<0.01）.Independent prognostic factors were gender（P=0.008），GTV（P=0.007），PVTT（P=0.006）and Child-Pugh class（P=0.003）.Conclusion Gender，GTV，PVTT and Child-Pugh class may be independent prognostic factors when using 3DCRT to treat PLC. Related Articles | Metrics

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Short-term therapeutic effect of re-operation on 246 patients with postoperative recurrent primary hepatic carcinoma WU Fei-Xiang, WANG Fang-Wei, MA Liang, HUANG Sheng-Xin, HUANG Shan, ZHAO Yin-Nong 2012, 4 (2):  167-170.  doi: 10.3969/j.issn.1674-5671.2012.02.17 Abstract ( 506 )   PDF (323KB) ( 615 )   Save Objective To explore the effects of different therapies in patients with postoperative recurrent primary hepatic carcinoma （PHC）. Methods Clinical data of 246 postoperative recurrent PHC patients were analyzed retrospectively.Of these，18 underwent hepatectomy again，20 underwent hepatectomy again combined with comprehensive therapy，62 were treated with transcatheter arterial chemoembolization（TACE），and 146 were given conservative therapy. Results Survival rates in the 1st，3rd and 5th years were 76.3%，39.4% and 25.8% for patients who underwent hepatectomy again，compared to 53.2%，19.6% and 9.3% for patients treated with TACE. The corresponding rates for patients given conservative therapy were 37.7%，8.0% and 1.5%.The survival rates differed significantly among the three groups（P＜0.05）. Conclusions Secondary surgical resection is still the preferred method for recurrent PHC，and surgical excision combined with comprehensive treatment is the best treatment.It is associated with higher survival rate and tumor-free survival，lower intrahepatic PHC relapse，and longer survival time. Related Articles | Metrics

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Establishment of a drug-resistant tongue cancer cell line and analysis of its cancer stem cell characteristics CHEN Xiang-Yi, KUANG Xiao-Cong, CAI Jie, TAO Lu 2012, 4 (2):  170-173.  doi: 10.3969/j.issn.1674-5671.2012.02.18 Abstract ( 496 )   PDF (501KB) ( 648 )   Save Objective To establish a drug-resistant tongue cancer cell line and analyze its cancer stem cell（CSC）characteristics. Methods Tongue cancer cell line TCA-8113 was induced by adriamycin（ADM）and used to establish the drug-resistant cell line TCA-8113/ADM.Basic characteristics of both the parental cell line TCA-8113 and the drug-resistant cell line TCA-8113/ADM were analyzed and compared， including cell growth，doubling time，cell cycle， resistance index（RI），single-cell culture，colony formation and formation of sphere cells. Results The growth pattern of TCA-8113/ADM cells was cobblestone-like， with cultures showing sig-nificantly more atypical cells than those of parental cells.TCA-8113/ADM cells had an RI of 22，and their rates of cell proliferation， cell doubling，colony formation and sphere cell formation were slower than those of the parental cells. Conclusion Sustained incre-mental exposure to low concentrations of ADM can induce a drug-resistant tongue cancer cell line，but its CSC characteristics are not obvious. Related Articles | Metrics

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Construction and expression of the recombinant plasmid BFP-cyclin D1 and its influence on proliferation and migration in breast cancer cells MCF-7 LI Yong-Ji, HUANG Yi, RUAN Xi-Ling, LIAO Liu-Feng, WU Qiong, HUANG Wen-Tao, XU Heng 2012, 4 (2):  174-178.  doi: 10.3969/j.issn.1674-5671.2012.02.19 Abstract ( 490 )   PDF (509KB) ( 467 )   Save Objective To construct the eukaryotic expression plasmid BFP-cyclin D1 and investigate the effects of cyclin D1 on the proliferation and migration of MCF-7 cells. Methods The cyclin D1 gene was amplified by RT-PCR from total RNA of MCF-7 cells， digested with EcoR I and Sal I， and inserted into the eukaryotic florescence expression vector pEBFP-N1.The resulting recombinant plasmid BFP-cyclin D1 was transfected into MCF-7 cells.In parallel，one negative control group was transfected with pEBFP-N1 and another with transfection reagent without any DNA.Following transfection，cell proliferation was detected by MTT assay，cell migration by cell scratch assay，and mRNA expression by quantitative real-time PCR. Results The eukaryotic expression plasmid BFP-cyclin D1 was successfully constructed and high expression of cyclin D1 was detected in MCF-7 cells.Cell proliferation and migration were significantly higher in the cells transfected with BFP-cyclin D1 than in the two control groups（P<0.01）. Conclusions High expression of cyclin D1 can accelerate proliferation and migration of MCF-7 cells，perhaps by shortening the cell cycle and promoting DNA replication. Related Articles | Metrics

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Establishment of a juvenile-onset recurrent respiratory papillomatosis（JO-RRP）transplanted tumor model in nude mice HAN Lin, TANG Chao-Xian, LI Jin-Ming, WU Sen-Yong, XIE Ying, WEN Wen-Sheng 2012, 4 (2):  179-183.  doi: 10.3969/j.issn.1674-5671.2012.02.20 Abstract ( 508 )   PDF (584KB) ( 431 )   Save Objective To establish a juvenile-onset recurrent respiratory papillomatosis（JO-RRP）transplanted tumor model in nude mice. Methods Fresh tissues of laryngeal papilloma were obtained from 36 juvenile patients during operations.Tissues were cut into small pieces of approximately 1 mm3，transplanted into BALB/c nude mice subcutaneously，and then observed periodically. Results Tumors were successfully transplanted in 20 mice，corresponding to a tumor formation rate of 56%.The transplanted tumors presented histological characteristics similar to those of the tumors in humans.Virus HPV6/11 was confirmed in the transplanted tumor tissues by in situ hybridization and PCR. Conclusion A transplanted tumor model of JO-RRP has been established，which should provide a basis for studying juvenile laryngeal papillomatosis. Related Articles | Metrics

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Expression and functional of VEGF and EPO in hypoxic metabolism during one-lung ven-tilation GE Wan-Yun, PAN Ling-Hui, LIN Fei 2012, 4 (2):  184-187.  doi: 10.3969/j.issn.1674-5671.2012.02.21 Abstract ( 439 )   PDF (280KB) ( 420 )   Save Objective To explore the expression and functional of the serum vascular endothelial growth factor（VEGF） and erythro-poietin（EPO）in hypoxic metabolism during one-lung ventilation（OLV）. Methods Patients undergoing intrathoracic radical resection of esophageal carcinoma were randomly divided into two groups：one in which patients underwent OLV（n = 17）and another in which pa-tients underwent two-lung ventilation（TLV；n = 20）.Blood samples were taken immediately before OLV/TLV（T1） and at 30 min（T2），60 min（T3），90 min（T4） and 120 min（T5） after the start of OLV/TLV.Venous blood was collected at the end of the surgery （T6） to measure expression levels of VEGF and EPO.HGB levels were assessed at T1，T3 and T6. Results PaO2 at T6 was significantly lower in the OLV group than in the TLV group（P＜0.05）.The OLV group showed significantly higher VEGF expression at T3，T4，T5 and T6，as well as significantly higher serum EPO expression at T4 and T5 than did the TLV group（P＜0.05）. Conclusion Expression of VEGF and EPO significantly increased during OLV，suggesting that they participate in the injury and/or healing of pulmonary tis-sue during hypoxia. Related Articles | Metrics

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Familial aggregation in first-degree relatives of 479 probands with hepatocellular carcinoma LIN Shu-Han, XIAO Jun, LI Le-Qun 2012, 4 (2):  187-190.  doi: 10.3969/j.issn.1674-5671.2012.02.22 Abstract ( 512 )   PDF (316KB) ( 433 )   Save Objective To investigate the prevalence of hepatocellular carcinoma（HCC）among first-degree relatives and explore the familial aggregation of HCC. Methods A cross-sectional study was performed on first-degree relatives of HCC probands treated at the Affiliated Cancer Hospital of Guangxi Medical University from 2004 to 2008.The binomial distribution model was applied to test the goodness-of-fit of the frequency distribution.Results There were 479 probands，accounting for 16.91%（479/2 832） of hospital admis-sions. Results of binominal distribution analysis showed that the number of HCC cases within proband families was significantly higher than the theoretical range（P=0.028）.The estimated separation ratio of HCC was 0.04，and the separation ratio variance was 0.000 015 09（SD=0.003 885，95%CI： 0.033 784～0.046 216）. Conclusion HCC shows significant familial aggregation in first-degree relatives of probands，and family history is one of the important risk factors for HCC.  Related Articles | Metrics

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Expression of hK2 and Ki-67 in prostate cancer and its clinical significance YU Yong, LI Dao-Yuan, LU Hao-Yuan, MENG Qing-Gui, BAI Xian-Zhong- 2012, 4 (2):  190-193.  doi: 10.3969/j.issn.1674-5671.2012.02.23 Abstract ( 675 )   PDF (414KB) ( 532 )   Save Objective To study the expression of hK2 and Ki-67 in prostate cancer and its clinical significance. Methods Expression of hK2 and Ki-67 was studied in tissues collected from 40 cases of prostate cancer（PCa） and 30 cases of benign prostatic hyperplasia（BPH） using the immunohistochemical SP method. Results The rates of hK2 and Ki-67 expression were significantly higher in PCa than in BPH （P<0.05）.Positive expression of hK2 in PCa tissue correlated with Gleason grading （P<0.05） but not with clinical stage（P>0.05），while positive expression of Ki-67 correlated with clinical stage（P<0.05） but not with Gleason grading（P>0.05）.Expression of hK2 positively correlated with expression of Ki-67（P<0.05）. Conclusions Abnormal expression of hK2 and Ki-67 correlated closely with the diagnosis and prognosis of PCa.Combined detection of hK2 and Ki-67 may be useful as a marker for early diagnosis and prognostic evaluation of PCa. Related Articles | Metrics

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Evaluation of axillary lymph node metastases using diffusion-weighted imaging LUO Ning-Bin, SU Dan-Ke, LIU Li-Dong, JIN Guan-Qiao, ZHU Xu-Na, XIE Dong 2012, 4 (2):  194-196.  doi: 10.3969/j.issn.1674-5671.2012.02.24 Abstract ( 591 )   PDF (341KB) ( 565 )   Save Objective To evaluate the ability of diffusion-weighted imaging（DWI） to diagnose axillary lymph node metastases in patients with breast cancer. Methods Preoperative conventional MR and DWI imaging was performed on 24 patients with breast can-cer and enlarged axillary lymph nodes.The apparent diffusion coefficient （ADC） of axillary lymph nodes was determined，and its rela-tionship to histopathologic findings was analyzed. Results The ADC value of metastatic lymph nodes （0.796±0.171） ×10-3mm2/s was significantly lower than that of benign nodes（1.058±0.234，P<0.05）×10-3mm2/s.With the threshold ADC value at 0.854×10-3mm2/s for diagnosing lymph node metastasis，the sensitivity，specificity and accuracy were，respectively，73.91%，86.96% and 80.43%. Conclusion DWI is useful for detecting axillary lymph node metastases in patients with breast cancer. Related Articles | Metrics

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Analysis of HPV type in high-grade cervical lesions OUYa-Meng-Lan , GAO Kun, LI Li 2012, 4 (2):  197-199.  doi: 10.3969/j.issn.1674-5671.2012.02.25 Abstract ( 662 )   PDF (275KB) ( 456 )   Save Objective To investigate the infection rate and type of human papillomavirus（HPV） in high-grade cervical squamous in-traepithelial lesions. Methods Types of HPV genes were detected using a gene-chip technique in 30 patients with CINⅡ-Ⅲ and 160 patients with cervical cancer.The relationship between HPV infection and cervical lesions was analyzed. Results Rates of HPV infec-tion in both groups were 90%，with 70.31%（19/27） and 81.94%（118/144） being single-type infections. HPV-58 and HPV-52 were the most frequent types among patients with CINⅡ-Ⅲ，while HPV-16 and HPV-18 were the most frequent types among patients with cervical cancer. HPV-16 was detected most frequently in both squamous cell carcinoma and adenocarcinoma. Conclusion HPV-16 and HPV-18 are the major HPV types associated with cervical cancer，while HPV-58 and HPV-52 are the major types associated with high-grade squamous intraepithelial lesions. Related Articles | Metrics

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Correlation of nutritional status and quality of life in patients with locally advanced nasopharyngeal carcinoma after concurrent radiotherapy and chemotherapy WEI Xia, CHEN Li-Jun 2012, 4 (2):  200-203.  doi: 10.3969/j.issn.1674-5671.2012.02.26 Abstract ( 507 )   PDF (281KB) ( 609 )   Save Related Articles | Metrics

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Epidermal growth factor gene polymorphism and risk of hepatocellular carcinoma: a Meta-analysis Zhong-Jian-Hong, YOU Xue-Mei, GONG Wen-Feng 2012, 4 (2):  204-204.  doi: 10.3969/j.issn.1674-5671.2012.02.27 Abstract ( 416 )   PDF (178KB) ( 424 )   Save Related Articles | Metrics