Abstract: Objective To observe the inhibitory effects of total flavonoids from Camellia Chrysantha （Hu） Tuyama on four malignant tumor cell lines in vitro. Methods Malignant tumor cell lines SMMC-7721，CNE-1，SGC-7901 and H460 were treated with total flavonoids， and inhibition of cell proliferation was measured using the MTT assay. Results Total flavonoids from Camellia Chrysantha （Hu） Tuyama were found to exert antiproliferative activity on all four tumor cell lines in a dose-dependent manner.IC₅₀ values（μg/ml） were 242.44 for SMMC-7721 cells，313.79 for CNE-1，259.87 for SGC-7901 and 385.87 for H460. Conclusion Total flavonoids from Camellia Chrysantha （Hu） Tuyama can inhibit cell proliferation of SMMC-7721，CNE-1，SGC-7901 and H460 in vitro.

Key words:  Flavonoids Camellia Chrysantha （Hu） Tuyama, Antitumor, MTT assay, Inhibition of proliferation