Abstract: Objective To clone the cDNA of the gene encoding special AT-rich sequence binding protein （SATB1） and to subclone it into eukaryotic expression vector pEGFP in order to investigate the function and mechanism of action of SATB1. Methods SATB1 cDNA was cloned from pCMV6-XL6-SATB1 by polymerase chain reaction （PCR） and then the target gene was subcloned into pGEM-T and subsequently into the eukaryotic expression vector pEGFP-N1 to give pEGFP-SATB1. Results The complete cDNA sequence of SATB1（2312 bp） was consistent with the reported sequence（Genbank BC001744.1）.Sequencing and restriction digest analysis re-vealed the target gene to be inserted in the expression vector in the correct orientation. Conclusion The SATB1 cDNA was success-fully cloned and used to construct recombinant eukaryotic expression vector pEGFP-SATB1.

Key words:  SATB1 gene, Gene cloning, Eukaryotic expression vector