Abstract:

Objective This study used bioinformatics to examine the genes and signaling pathways associated with RhoGDP dissociation inhibitor 2（RhoGDI2） inhibits bladder cancer metastasis. Methods Six datasets from genomic microarray studies of patients with metastatic bladder cancer were downloaded from the Gene Expression Omnibus database. On-line GEO2R tools were used to screen for genes differentially expressed between individuals expressing low or high levels of RhoGDI2. On-line DAVID tools were used to map differentially expressed genes to signaling pathways. Result The genes A2M，CORO1A，ENC1，and FGD3 were expressed at higher levels in patients expressing high levels of RhoGDI2. RhoGDI2 appeared to influence the RhoA-ROCK-MLC and Ras-Raf- MEK signaling pathways to the greatest extent. Conclusion RhoGDI2 may act via the RhoA-ROCK-MLC and Ras-Raf-MEK pathways to regulate cytoskeleton， cell proliferation， differentiation and metastasis in bladder cancer.

Key words: Bladder neoplasm, Rho GDP dissociation inhibitor 2, Gene, Signaling pathways, Bioinformatics