Abstract:

Objective To explore the expression of CXC chemokine receptor-4 （CXCR4），phosphatidylinositol 3-kinase （PI3K） and AKT in the triple-negative breast cancer cell line MDA-MB-231. Methods MDA-MB-231 cells were transfected with pcDNA3.1 encoding CXCR4 or treated with siRNA against CXCR4. qRT-PCR and Western blotting were used to detect mRNA and protein expression of CXCR4，PI3K and AKT. Results Transfecting cells with pcDNA3.1-CXCR4 increased mRNA and protein levels of CXCR4，PI3K and AKT （P＜0.05）；siRNA-mediated silencing of CXCR4 reduced mRNA and protein levels of CXCR4，PI3K and AKT（P<0.05）. Conclusion CXCR4 expression may regulate the expression of PI3K and AKT in triple-negative breast cancer.

Key words: Breast neoplasms, Triple-negative breast cancer, CXC chemokine receptor-4, Phosphatidylinositol 3-kinase, AKT, Expression