Abstract: Objective To investigate the effect of silencing the gene of VEGF on the migration and invasion of radioresistant human nasopharyngeal carcinoma cell line CNE-2R. Methods Three groups of lentiviral vectors containing VEGF-shRNA （VEGF-LV1，VEGF-LV2，VEGF-LV3） and control shRNA recombinant lentivirus（VEGF-NC） were prepared. Cells of radio resistant human nasopharyngeal carcinoma CNE-2R were transfected to establish a cell line stably silencing VEGF expression（CNE-LV1，CNE-LV2，and CNE-LV3），and a negative control group（NE-NC） was established. RT-qPCR and Western blot were used to detect the infection effect of each group. Scratch test and Transwell chamber assay were used to detect cell migration and invasion after VEGF silencing. Results VEGF-shRNA recombinant lentivirus were successfully constructed and the stable subcellular cell line（CNE-LV3）with VEGF silencing was established. The scratch test showed that the cell migration rate of the CNE-LV3 group was significantly lower than that of the blank control group and the CNE-NC group［（37.97±1.56）% vs （50.17±1.76）%，P＝0.001；（37.97±1.56）% vs （50.63±1.52）%，P＝0.001］. Transwell chamber migration assay showed that the number of transmembrane cells in the CNE-LV3 group was significantly decreased compared with the blank control group and CNE-NC group（41.3±1.5 vs 95.3±3.8，P＜0.001； 41.3±1.5 vs 94.3±4.0，P＜0.001）. And Transwell chamber invasion assay also showed that the number of transmembrane cells in the CNE-LV3 group was significantly decreased compared with the blank control group and CNE-NC group（46.3±2.1 vs 105.3±1.5，P＜0.001；46.3±2.1 vs  93.3±2.5，P＜0.001）. Conclusion Silencing VEGF expression can inhibit the migration and invasion of radioresistant human nasopharyngeal carcinoma cell line CNE-2R.

Key words: Nasopharyngeal carcinoma, VEGF, Lentivirus, Radioresistant, Invasion, Migration