Table of Content

25 December 2019, Volume 11 Issue 6 Previous Issue    Next Issue

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Application of omics technology in tumor driver gene research PEI Zhiyong 2019, 11 (6):  447-453.  doi: 10.3969/j.issn.1674-5671.2019.06.01 Abstract ( 224 )   PDF (2962KB) ( 557 )   Save Related Articles | Metrics

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Exploring the effect of Her-2 gene expression on immune cell infiltration in gastric cancer based on bioinformatics method ZHANG Jing, YOU Lukuan, HU Yi 2019, 11 (6):  454-460.  doi: 10.3969/j.issn.1674-5671.2019.06.02 Abstract ( 243 )   PDF (25407KB) ( 70 )   Save Objective To explore the effect of human epidermal growth factor receptor-2 （Her-2） on immune cell infiltration in gastric cancer tissues and its possible mechanism. Methods The RNA-Seq data of 354 patients with complete information were downloaded from the TCGA. The CIBERSORT algorithm was used to investigate the proportion of infiltrating immune cells in the microenvironment of gastric cancer，and its correlation with Her-2 expression was analyzed to screen the potential types of Her-2 genes regulating infiltrating immune cells. Gene set variation analysis（GSVA） was used to evaluate the set of signal pathways related to Her-2 expression in gastric cancer tissues，and gastric transcriptome data collected by Gene Expression Omnibus（GEO） was used to further verify the relationship between Her-2 gene expression and the degree of gastric cancer immune cell infiltration. Results CIBERSORT analysis of gastric cancer samples from the TCGA database found that CD8+ T cells，resting memory CD4+ T cells，M0 macrophages，and mast cells infiltrated differentially in Her-2 high- and low-expression groups（P=0.029，0.049，0.027，0.008）. High expression of Her-2 gene was related to the development，induction，differentiation，and migration regulation of CD8+ T cells，resting memory CD4+ T cells，M0 macrophages and mast cells（P<0.05），and affected the infiltration capacity and proportion of some immune cells in gastric cancer tissues. Conclusion High expression of Her-2 gene in gastric cancer may have an inhibitory effect on the formation and function of some immune cells. Related Articles | Metrics

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Effect of proteasome inhibitor bortezomib on the growth of esophageal squamous cell carcinoma NIU Xia, ZHANG Xiaojuan, BI Yanghui, ZHANG Ling 2019, 11 (6):  461-466.  doi: 10.3969/j.issn.1674-5671.2019.06.03 Abstract ( 348 )   PDF (7863KB) ( 71 )   Save Objective To investigate the effect of proteasome inhibitor bortezomib on the growth of esophageal squamous cell carcinoma（ESCC）. Methods Based on whole genome sequencing data，DGIdb database was used to screen esophageal squamous cell carcinoma-related druggable genes，and KEGG signal pathway enrichment analysis was performed using DAVID software. The effect of bortezomib on the growth of 5 ESCC cells including KYSE30，KYSE180，KYSE150，TE1and KYSE510 were measured by MTT，and DMSO-treated cells were used as the corresponding control group. Nude mice were injected intraperitoneally with saline （control group） and bortezomib （bortezomib group） after tumor formation in vitro. The effects of bortezomib on the volume and weight of xenografts in nude mice were observed.Immunohisto-chemistry was performed to determine the expression of Ki-67 protein in xenograft tumor tissues treated with bortezomib. Results A total of 307 druggable genes were identified from genomics sequencing data of 469 ESCC cases in the DGIdb database，and the significantly mutated druggable genes with mutation frequencies>2.5% included PIK3CA，NOTCH1，CDKN2A，ERBB4，etc.The enriched signaling pathways of all druggable genes included RTK-RAS，PI3K/AKT/mTOR，NOTCH，ERBB signaling pathway，cell cycle and proteasome pathway，etc. MTT results showed that the proliferation of 5 ESCC cell lines treated with bortezomib were significantly inhibited compared with control group（P<0.05）. Compared with the control group，the tumor volume of the bortezomib group was significantly decreased［（1 909.18±533.40） mm3 vs （1 065.83±283.94） mm3，P<0.01］，and the tumor weight of the bortezomib group was also significantly decreased ［（1.60±0.36） g  vs （0.98±0.30） g，P<0.01］. Immunohistochemistry showed that the expression of Ki-67 in the bortezomib group was significantly decreased compared with the control group （43.83±3.22  vs 86.32±4.51，P<0.01）. Conclusion Bortezomib can inhibit the cell growth of ESCC in vitro and in vivo. Related Articles | Metrics

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Pan-cancer integration study on miR-200 family memberstargeted heterogeneity in different tumors TANG Yong, LIU Haizhou, CHEN Xi, WU Jianyong, LIU Yingzhao, YANG Linkai, WANG Qi 2019, 11 (6):  467-473.  doi: 10.3969/j.issn.1674-5671.2019.06.04 Abstract ( 248 )   PDF (37180KB) ( 60 )   Save Objective To investigate the effect of miR-200 family members on the prognosis of different tumors and the mechanism of target gene regulation heterogeneity. Methods The transcriptome data and clinical phenotypes of 21 different histopathological malignant solid tumor samples from the PanCanAtlas were downloaded from the Cancer Genome Atlas（TCGA），and the relationship between miR-200 family members and tumor prognosis were analyzed. Five tumors with contradictory survival risk assessment results were further selected for WGCNA cluster analysis of miR-200 family target genes.The expression of target genes significantly related to miR-200 family expression in bladder cancer tissues was verified by immunohistochemistry and fluorescent quantitative PCR，and their effects on patient survival were analyzed. Results The high expression of miR-200 family was a protective factor for the prognosis of bladder cancer and gastric adeno-carcinoma（HR=0.55~0.68，P=0.0001~0.0340；HR=0.61~0.72，P=0.0055~0.0170），and a negative factor for the prognosis of liver cancer and thymoma（HR=1.46~1.65，P=0.0061~0.0120；HR=7.44~13.04，P=0.0002~0.0210），while miR-141 and miR-429 had opposite effects on the survival of renal papillary cell carcinoma. Furter clustering of miR-200 family target gens showed that the expression of TIMP2 gene increased the survival time of bladder cancer patients，and miR-141 prolonged the survival of the patients with renal papillary cell carcinoma by negatively regulated EPHA2 gene. TIMP2 protein expression in bladder cancer was negatively correlated with miR-200 family expression and patients with high expression had better prognosis. Conclusion miR-200 family had different effects on the prognosis of different tumors，and the heterogeneity of miR-200 family targeted molecules results to the heterogeneity of biological function of miR-200 family in cancers. Related Articles | Metrics

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Effect of silencing VEGF on the invasion and migration of radioresistant human nasopharyngeal carcinoma cell line CNE-2R CHEN Li, LIN Guoxiang, CHEN Kaihua, SUN Yongchu, WAN Fangzhu, ZHU Xiaodong 2019, 11 (6):  474-479.  doi: 10.3969/j.issn.1674-5671.2019.06.05 Abstract ( 266 )   PDF (16969KB) ( 63 )   Save Objective To investigate the effect of silencing the gene of VEGF on the migration and invasion of radioresistant human nasopharyngeal carcinoma cell line CNE-2R. Methods Three groups of lentiviral vectors containing VEGF-shRNA （VEGF-LV1，VEGF-LV2，VEGF-LV3） and control shRNA recombinant lentivirus（VEGF-NC） were prepared. Cells of radio resistant human nasopharyngeal carcinoma CNE-2R were transfected to establish a cell line stably silencing VEGF expression（CNE-LV1，CNE-LV2，and CNE-LV3），and a negative control group（NE-NC） was established. RT-qPCR and Western blot were used to detect the infection effect of each group. Scratch test and Transwell chamber assay were used to detect cell migration and invasion after VEGF silencing. Results VEGF-shRNA recombinant lentivirus were successfully constructed and the stable subcellular cell line（CNE-LV3）with VEGF silencing was established. The scratch test showed that the cell migration rate of the CNE-LV3 group was significantly lower than that of the blank control group and the CNE-NC group［（37.97±1.56）% vs （50.17±1.76）%，P＝0.001；（37.97±1.56）% vs （50.63±1.52）%，P＝0.001］. Transwell chamber migration assay showed that the number of transmembrane cells in the CNE-LV3 group was significantly decreased compared with the blank control group and CNE-NC group（41.3±1.5 vs 95.3±3.8，P＜0.001； 41.3±1.5 vs 94.3±4.0，P＜0.001）. And Transwell chamber invasion assay also showed that the number of transmembrane cells in the CNE-LV3 group was significantly decreased compared with the blank control group and CNE-NC group（46.3±2.1 vs 105.3±1.5，P＜0.001；46.3±2.1 vs  93.3±2.5，P＜0.001）. Conclusion Silencing VEGF expression can inhibit the migration and invasion of radioresistant human nasopharyngeal carcinoma cell line CNE-2R. Related Articles | Metrics

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Detection of mitochondrial DNA mutations in hepatocellular carcinoma by targeted next generation sequencing LIU Yang, GUO Shanshan, LIU Manling, LI Yijie, JIA Yongfeng, GUO Xu 2019, 11 (6):  480-484.  doi: 0.3969/j.issn.1674-5671.2019.06.06 Abstract ( 190 )   PDF (3163KB) ( 237 )   Save Objective To investigate mitochondrial DNA（mtDNA） mutations between different types of samples in hepatocellular carcinoma （HCC） patients. Methods The tumor tissues，adjacent tissues （≤3 cm from the tumor tissue），peripheral blood cells，and plasma of 5 patients with HCC diagnosed pathologically in the First Affiliated Hospital of Air Force Medical University were collected from September 23，2017 to December 18，2017. mtDNA copy number of tumor tissues and plasma samples were detected by whole genome sequencing. mtDNA mutations in tumor tissues，adjacent tissues，peripheral blood cells and plasma samples were analyzed by targeted next generation sequencing（NGS）. Results Whole genome sequencing results showed that the mtDNA copy number in plasma was significantly reduced compared with tumor tissues（Z＝-2.023，P＝0.008）. Targeted capture sequencing results showed that 181，180，181，179 homogeneous mutations and 33，42，3，78 heterogeneous mutations were identified in tumor tissues，adjacent tissues，peripheral blood cells and plasma samples，respectively. 10，39，and 56 specific mutation sites were detected in tumor tissues，adjacent tissues，and plasma，respectively. The frequency of mtDNA mutations in plasma was not significantly lower than that in tumor tissues（P=0.219）. Conclusion  Targeted next generation sequencing can detect mtDNA mutations in plasma samples of patients with hepatocellular carcinoma，and accurately discover the low-frequency mutations in plasma，which can be a powerful complement to tissue biopsy. Related Articles | Metrics

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Effect of ellagic acid on the biological behaviors of BRCA1  silenced triple negative breast cancer cell line MDA-MB-231 and its possible machanism WEI Liuxia, HE Meiling, HUANG Junqing, HUANG Shaoxin, LIANG Li, HE Lifeng, ZHANG Yumei 2019, 11 (6):  485-491.  doi: 10.3969/j.issn.1674-5671.2019.06.07 Abstract ( 329 )   PDF (5760KB) ( 277 )   Save Objective To investigate the effects of ellagic acid（EA） on the biological behaviors of breast cancer susceptibility gene 1（BRCA1） silenced in triple negative breast cancer cell line MDA-MB-231 and its possible mechanism. Methods Different concentrations of EA （0 μg/mL，1 μg/mL， 5 μg/mL，10 μg/mL and 20 μg/mL） were used to treat MDA-MB-231 cells for 24 h，48 h and 72 h，respectively. The inhibition rate of proliferation were assessed by MTT assay and the IC50 was calculated to select the optimal drug concentration. After transfecting BRCA1 siRNA into MDA-MB-231 cells，EA was applied at a final concentration of 5 μg/mL for 0 h，24 h，48 h，72 h，96 h，and cell proliferation was detected by MTT assay. Scratch assay was used to detect cell migration，Transwell chamber was used to detect cell migration and invasion，RT-qPCR and Western blot were used to detect the expression of PARP1 mRNA and protein. Results Different concentrations of EA had significant inhibitory effect on the proliferation of MDA-MB-231 cells at 24 h，48 h and 72 h，and IC50 were 13.739 μg/mL，10.645 μg/mL，5.344 μg/mL，respectively. The OD value in siRNA+EA group was significantly lower than that of control group，EA group and negative control group（P<0.001）. The cell migration rate and the number of transmembrane cells in siRNA+EA group were lower than those of the other three groups（P<0.001），and the expression of PARP1 mRNA and protein in siRNA+EA group were also significantly lower than those of the other three groups（P<0.001）. Conclusions EA can inhibit the proliferation，migration and invasion of BRCA1 silenced MDA-MB-231 cells in vitro，and its mechanism may be related to related DNA repair pathway disorders. Related Articles | Metrics

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Expression and significance of TFB1M in colorectal cancer YANG Shirong, CHEN Yanqin, BAI Xiangyu, GAN Lu, HE Xianl 2019, 11 (6):  492-497.  doi: 10.3969/j.issn.1674-5671.2019.06.08 Abstract ( 344 )   PDF (17780KB) ( 67 )   Save Objective  To investigate the expression mitochondrial transcription factor B1（TFB1M） in colorectal cancer tissue and its significance. Methods A total of 112 cases of surgical resection of patients with colorectal cancer and their clinical information were collected，and the expression level of TFB1M in colorectal cancer tissues and paired adjacent normal tissues were detected by Immunohistochemistry. The transcriptome data and clinical information were downloaded from the Cancer Genome Atlas（TCGA） database，and the relationship between the expression of TFB1M and clinical pathological characteristics and prognosis of patients with colorectal cancer. The cell proliferation was determined by MTS assay after interfering and overexpressing TFB1M in SW620 and HCT116 cells. Results The positive expression score of TFB1M in colorectal cancer tissues was significantly lower than that in adjacent normal tissues［（3.79±1.66） points vs （8.14±1.82） points，P<0.01］ TFB1M expression in stage Ⅲ+Ⅳ patients with colorectal cancer were significantly lower than stage Ⅰ+Ⅱ patients through analyzing TCGA database and clinical information. Furthermore，the overall survival of patients with high expression of TFB1M was better than that of patients with low expression（P<0.01），and TFB1M was an independent factor affecting the overall survival of patients with colorectal cancer. After interfering with TFB1M expression，the proliferation of colorectal cancer SW620 and HCT116 cells were significantly enhanced compared with the control group，while the proliferation of overexpressing TFB1M cells was decreased. Conclusions Low TFB1M expression has a poor prognosis. Interfering with TFB1M can promote colorectal cancer cell proliferation. TFB1M may be used as a biomarker for predicting the prognosis of colorectal cancer patients. Related Articles | Metrics

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Establishment and verification of a scoring system for predicting central lymph node metastasis in papillary thyroid carcinoma  WU Yuanyuan, WANG Jun, MAO Aihong, FAN Xiangda, ZHANG Jianwei, DUAN Ying 2019, 11 (6):  498-502.  doi: 10.3969/j.issn.1674-5671.2019.06.09 Abstract ( 374 )   PDF (1377KB) ( 215 )   Save Objective  To establish a scoring system for predicting central lymph node metastasis （CLNM） in papillary thyroid carcinoma （PTC），then evaluated and verified its work efficiency. Methods Totals of 237 patients with PTC who underwent surgery in Gansu Province Cancer Hospital from June 2015 to June 2018 were selected as the modeling group，and 100 patients met the same criteria from July 2018 to June 2019 were selected as the verification group. Logistic regression model was used to screen independent risk factors and establish scoring system for predicting CLNM，and ROC curve was used to evaluate the predictive value of the scoring system. Results Male，tumor diameter＞1 cm，multifocality，lower location，and capsular invasion were independent predictive factors for CLNM. The area under ROC curve of the scoring system in modeling group was 0.857，and the best cutoff value for the diagnosis of CLNM was 6 points with sensitivity of 81.9%，specificity of 85.3%，positive predictive value of 78.6% and negative predictive value of 87.8%. The area under ROC curve of the verification group was 0.823. Conclusions The scoring system base on independent risk factors has good predictive value of CLNM in PTC. Prophylactic central neck dissection should be actively performed when the PTC patients with score ≥6 points have a high risk of CLNM. Related Articles | Metrics

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Effect of C-reactive protein to albumin ratio on prognosis of P-Gemox regimen combined with radiotherapy in patients with early extranodal NK/T cell lymphoma CHEN Xiaoyan, QUAN Xiaoying, LI Li 2019, 11 (6):  503-508.  doi: 10.3969/j.issn.1674-5671.2019.06.10 Abstract ( 347 )   PDF (6822KB) ( 320 )   Save Objective To explore the prognostic significance of C-reactive protein to albumin ratio for（CRP/Alb） extranodal NK/T cell lymphoma（ENKTL） treated with P-Gemox chemotherapy regimen combined radiotherapy. Methods The clinical data of 83 ENKTL patients treated in Sichuan Cancer Hospital from 2012 to 2017 were retrospectively analyzed. X-Tile software was used to calculate the cutoff value of CRP/Alb. According to the cutoff value，patients were divided into low CRP/Alb group（CRP/Alb<0.4，n=64） and high CRP/Alb group（CRP/Alb≥0.4，n=19）. Cox regression was used to analyze the relationship between CRP/Alb and prognosis. Results The 3-year progression-free survival（PFS） rate in the low CRP/Alb group was higher than that in the high CRP/Alb group（77.1% vs 30.7%，χ2=20.954，P<0.001），and the 3-year overall survival（OS） rate was also higher than in the high CRP/Alb group（87.4% vs 36.1%，χ2=22.559，P<0.001）. Multivariate Cox regression showed that CRP/Alb≥0.4 was an independent risk factor for PFS（HR=4.163，95%CI：1.899-9.128，P<0.001） and OS（HR=4.424，95%CI：2.026-9.662，P<0.001） in ENKTL patients. Further analysis showed that the median PFS and median OS of the low CRP/Alb group were higher than those of the high CRP/Alb group in both low-risk ENKTL patients and medium-high risk ENKTL patients（P<0.05）. Conclusion  The prognosis of patients with high CRP/Alb in early ENKTL treated with P-Gemox combined with radiotherapy is poor. Related Articles | Metrics

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Relationship between system immune-inflammation index and prognosis of advanced non-small cell lung cancer GUO Jing, WANG Youjun, CHU Huisong, QU Jie, ZHAO Ruhan, ZHOU Xin, LYU Xiying 2019, 11 (6):  508-512.  doi: 10.3969/j.issn.1674-5671.2019.06.11 Abstract ( 477 )   PDF (2811KB) ( 383 )   Save Objective To investigate the value of system immune-inflammation index（SII） in the prognosis of patients with advanced non-small cell lung cancer（NSCLC）. Methods Clinical data of 300 patients with advanced NSCLC who were admitted to the Chengde Medical College Affiliated Hospital from October 2013 to December 2018 were collected. ROC curve was used to calculate the best SII cutoff value，and patients were divided into high SII group（SII≥767.24×109，n=168） and low SII group（SII＜767.24×109，n=168）according to the best cutoff value . The clinicopathological characteristics and overall survival of the two groups of patients were compared，and the relationship between SII and prognosis was analyzed by Cox regression. Results SII was associated with clinical staging and KPS score（P<0.05）. The median survival in the low SII group was higher than that in the high SII group（21 months vs 13 months，χ2=16.824，P<0.001）.Univariate Cox regression showed that pathological type，clinical stage，smoking，KPS score，SII，Hb level and treatment regimen were all associated with the prognosis of advanced NSCLC patients（P<0.05）. Multivariate Cox regression showed that SII≥767.24×109 was an independent risk factor for the overall survival of advanced NSCLC patients（HR=1.416，95%CI：1.039-1.930，P=0.028）. Conclusion High SII is an independent risk factor for the overall survival of patients with advanced NSCLC，which can be used as a prognostic indicator. Related Articles | Metrics

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Expression of ERCC1,TYMS and RRM1 in cervical cancer tissues and their relationship with chemotherapy effect ZHOU Wen, WANG Jiali, TAN Bibo, MO Lingzha 2019, 11 (6):  513-517.  doi: 1674-5671（2019）06-0513-05 Abstract ( 222 )   PDF (651KB) ( 197 )   Save Objective To investigate the expression of nucleotide excision repair cross complementary gene 1（ERCC1），thymidylate synthetase gene（TYMS） and nucleotide reductase M1（RRM1） in cervical cancer and their relationship with clinicopathological feactures and chemotherapy effect. Methods The clinical data of 33 patients with cervical cancer diagnosed by pathology from January 2016 to December 2016 were collected. The expression of ERCC1，TYMS and RRM1 mRNA in cervical cancer tissues was detected by RT-PCR，and the relationship between ERCC1，TYMS and RRM1 mRNA，clinicopathological features and chemotherapy effect were analyzed. Results The positive rates of ERCC1，TYMS and RRM1 in 33 cases of cervical cancer tissues were 78.8%，69.7% and 75.8%，respectively. The expression of ERCC1 was related to FICG stage，tumor size，high-risk HPV infection，lymphangio-vascular tumor thrombus，myometrial invasion depth，squamous cell carcinoma antigen（SCC-Ag）（P<0.05）； the expression of TYMS was related to tumor size，high-risk HPV infection，lymphangio-vascular tumor thrombus（P<0.05）； the expression of RRM1 was not related to clinicopathological features（P>0.05）. The expression of ERCC1 and TYMS mRNA was positive correlated（r=0.608， P=0.004）. The total effective rate of ERCC1，TYMS and RRM1 positive patients were lower than those of negative patients（P<0.05），and the 3-year overall survival rate were lower than those of negative patients，but the difference was not statistically significant（P>0.05）. Conclusions The patients with ERCC1，TYMS or RRM1 positive expression in cervical cancer have poor chemotherapy effect. Related Articles | Metrics

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Expression and correlation of GPRC5A,SOCS3 and STAT3 in colorectal cancer tissues  YUAN Jianhua, ZHANG Shubo, LI Jianwang, MAO Shanshan, CUI Ronghua, ZHANG Gang 2019, 11 (6):  518-522.  doi: 10.3969/j.issn.1674-5671.2019.06.13 Abstract ( 323 )   PDF (2939KB) ( 176 )   Save Objective To explore the expression of G protein-coupled receptor，family C，group 5，member A（GPRC5A），signal transducer and activator of transcription 3（STAT3） and suppressor of cytokine signaling 3（SOCS3） in colorectal cancer tissues and their relationsip with clinicopathological features. Methods Totals of 76 colorectal cancer tissues  and 30 adjacent normal tissues were enrolled. Immunohistochemistry was used to detect the expressions of GPRC5A，SOCS3 and STAT3 in different tissues，and the relation ship between the expression and clinicopathological features and their correlation were analyzed by spearman correlation analysis. Results The positive expression rates of GPRC5A and STAT3 in colorectal cancer tissues were significantly higher than those in adjacent normal tissues（59.2% vs 33.3%，P＝0.016；69.7% vs 30.0%，P＝0.001），but the positive expression rate of SOCS3 was significantly lower than that in adjacent normal tissues（38.2% vs 80.0%，P＝0.001）. The expression of GPRC5A was significantly associated with pathological differentiation，lymph node metastasis，and TNM staging of colorectal cancer（P<0.05）. The expression of SOCS3 and STAT3 were significantly associated with pathological differentiation，depth of invasion，lymph node metastasis，and TNM staging of colorectal cancer（P<0.05）. The Spearman correlation analysis showed that SOCS3 protein expression were negatively correlated with STAT3 expression and GPRC5A expression（r=－0.308，P=0.007；r=-0.340，P=0.003），and GPRC5A protein expression was positively correlated with STAT3（r=0.327，P=0.004）. Conclusions GPRC5A is highly expressed in colorectal cancer tissues and may negatively regulate the expression of SOCS3 and positively regulate the expression of STAT3. GPRC5A，SOCS3 and STAT3 may be closely related to the occurrence and development of colorectal cancer. Related Articles | Metrics

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Application effect of ultrasound-guided transverse abdominal planar block combined with general anesthesia in the laparoscopic radical resection of colorectal cancer JU Feng, ZHANG Xianjie, ZHAO Zhifu 2019, 11 (6):  523-527.  doi: 10.3969/j.issn.1674-5671.2019.06.14 Abstract ( 221 )   PDF (638KB) ( 79 )   Save Objective To explore the clinical application effect of ultrasound-guided transverse abdominal planar block combined with general anesthesia in the laparoscopic radical resection of colorectal cancer. Methods From June 2017 to June 2019，100 patients undergoing laparoscopic radical resection of colorectal cancer in Deyang People's Hospital were selected，and divided into control group（n=50） and observation group（n=50） according to the random number table. The control group was given general anesthesia，and on this basis，the observation group was given ultrasound-guided horizontal abdominal block. The hemodynamics，analgesic effect and adverse reactions were compared between the two groups. Results The mean arterial pressure（MAP） and heart rate（HR） at the time of skin incision，resection of cancer focus and the end of operation，dosage of propofol and sufentanil and the time of opening eyes，extubating trachea and fully awakening，the VAS scores at 2，12，24，48 and 72 hours after operation in observation group were significantly lower than those in control group（P<0.05）. The times of analgesia pump and remedial analgesia in observation group were significantly lower than those in control group（P<0.05），but the satisfactory rate of analgesia in observation group was significantly higher than that in control group（96.0% vs 82.0%，χ2=5.005，P=0.025）. There was no significant difference in the incidence of adverse reactions between control group and observation group（10.0% vs 14.0%，χ2=0.379，P=0.538）. Conclusions  Ultrasound-guided transverse abdominal planar block combined with general anesthesia can effectively improve the anesthesia effect of patients undergoing laparoscopic radical resection of colorectal cancer，stabilize hemodynamics，reduce dosage of general anesthesia，and has better sefety，which is worthy of clinical promotion and application. Related Articles | Metrics

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Research progress of lactate dehydrogenase C4 and malignant tumors LIN Yingfeng, ZHOU Feng, CHEN Yan, CUI Zhaolei 2019, 11 (6):  528-531.  doi: 10.3969/j.issn.1674-5671.2019.06.15 Abstract ( 422 )   PDF (554KB) ( 284 )   Save Related Articles | Metrics

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Research progress of ANRASSF1 and tumors  HUANG Xiancong, WANG Changchun, MAO Weimin 2019, 11 (6):  532-535.  doi: 10.3969/j.issn.1674-5671.2019.06.16 Abstract ( 214 )   PDF (511KB) ( 186 )   Save Related Articles | Metrics

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Research progress on the relationship between peripheral blood inflammatory markers and prognosis of diffuse large B-cell lymphoma HUANG Xin, CEN Hong 2019, 11 (6):  536-540.  doi: 10.3969/j.issn.1674-5671.2019.06.17 Abstract ( 360 )   PDF (614KB) ( 268 )   Save Related Articles | Metrics

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Cardiotoxicity related to the therapy of breast cancer and its prevention and treatment FENG Yanying 2019, 11 (6):  540-544.  doi: 10.3969/j.issn.1674-5671.2019.06.18 Abstract ( 452 )   PDF (660KB) ( 304 )   Save Related Articles | Metrics

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Efficacy of concurrent chemoradiotherapy for poorly differentiated nasopharyngeal adenocarcinoma: a case report LI Ye, LIN Manru, HAN Yuyuan, DU Youqin, HUANG Shiting, MA Jialin, QU Song, LI Ling 2019, 11 (6):  544-546.  doi: 10.3969/j.issn.1674-5671.2019.06.19 Abstract ( 380 )   PDF (1424KB) ( 297 )   Save Related Articles | Metrics