Abstract: Objective  To investigate the expression mitochondrial transcription factor B1（TFB1M） in colorectal cancer tissue and its significance. Methods A total of 112 cases of surgical resection of patients with colorectal cancer and their clinical information were collected，and the expression level of TFB1M in colorectal cancer tissues and paired adjacent normal tissues were detected by Immunohistochemistry. The transcriptome data and clinical information were downloaded from the Cancer Genome Atlas（TCGA） database，and the relationship between the expression of TFB1M and clinical pathological characteristics and prognosis of patients with colorectal cancer. The cell proliferation was determined by MTS assay after interfering and overexpressing TFB1M in SW620 and HCT116 cells. Results The positive expression score of TFB1M in colorectal cancer tissues was significantly lower than that in adjacent normal tissues［（3.79±1.66） points vs （8.14±1.82） points，P<0.01］ TFB1M expression in stage Ⅲ+Ⅳ patients with colorectal cancer were significantly lower than stage Ⅰ+Ⅱ patients through analyzing TCGA database and clinical information. Furthermore，the overall survival of patients with high expression of TFB1M was better than that of patients with low expression（P<0.01），and TFB1M was an independent factor affecting the overall survival of patients with colorectal cancer. After interfering with TFB1M expression，the proliferation of colorectal cancer SW620 and HCT116 cells were significantly enhanced compared with the control group，while the proliferation of overexpressing TFB1M cells was decreased. Conclusions Low TFB1M expression has a poor prognosis. Interfering with TFB1M can promote colorectal cancer cell proliferation. TFB1M may be used as a biomarker for predicting the prognosis of colorectal cancer patients.

Key words: Colorectal cancer, TFB1M, The Cancer Genome Atlas, Expression, Prognosis