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25 March 2011, Volume 3 Issue 1 Previous Issue    Next Issue

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Relationship between codon 249 mutation and protein expression of p53 in hepatitis B virus and aflatoxin B1 related hepatocellular carcinoma Chen-Zhao-Hong, LI Le-Qun, QI Lu-Nan 2011, 3 (1):  1-5.  doi: doi：10.3969 / j.issn.1674-5671.2011.01.01 Abstract ( 420 )   PDF (1549KB) ( 752 )   Save 【Abstract】Objective To explore the mutation of p53 gene at codon 249 and the expression of p53 protein in hepatitis B virus (HBV) and aflatoxin B1 (AFB1) related hepatocellular carcinoma (HCC) Methods AFB1DNA adducts were detected by IHG special immunohistochemistry in 55 cases of pathologically confirmed HCC tissues and 10 cases of normal liver tissues Polymerase chain reaction (PCR) and direct sequencing were applied to detect p53 mutations at codon 249，and immunohistochemical SP method was applied to detect the expression of p53 protein Results The mutation rates of codon 249 in group A (HBV+ AFB1+) and C (HBV AFB1+) were 6875% (22/32) and 6364% (7/11) respectively，which were higher than those in group B (HBV+ AFB1，16.67%，2/12) and group D (normal control，0.00%，0/10) The expression rate of p53 in the cases with mutation at codon 249 was 9286% (26/28)，which was higher than that without the mutation (60.87%，16/27) and the difference was statistically significant Conclusions The mutation at codon 249 of p53 gene and AFB1 exposure are closely related during the process of HCC The rate of codon 249 mutation is higher when the patients exposing to both HBV and AFB1 The mutation of p53 gene may be one of the important factors for causing p53 protein expression in HCC   Related Articles | Metrics

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The impact of p53 on POLD1 in SMMC-7721 cells LIU Qi-Li, TAN Xiao-Hong, XU Heng, WEI Chang-Yuan, HONG Wei 2011, 3 (1):  5-9.  doi: doi：10.3969/j.issn.1674-5671.2011.01.02 Abstract ( 414 )   PDF (970KB) ( 556 )   Save ObjectiveTo investigate the impact of p53 on POLD1 gene in human liver cancer cell line SMMC-7721Methods Designed and constructed recombinant plasmid p53-siRNA and pEGFP-p53, then they  were transfected stably into SMMC-7721 cellsThrough G418 selection, the stable cell line 7721-p53，7721-p53RNAi  were acquiredThe p53 -RNA and POLD1 mRNA in these cell lines were detected by RT-PCR after transfectionResults High expression of wildtype p53 gene inhibited the transcription of POLD1 gene in SMMC-7721 cells, while low expression of p53 gene promoted the transcription of POLD1 geneConclusion p53 gene can regulate the transcription of POLD1 gene in SMMC-7721 cells Related Articles | Metrics