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25 December 2014, Volume 6 Issue 4 Previous Issue    Next Issue

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Treatment of high grade glioma: the present and future LANG Jin-yi 2014, 6 (4):  323-326.  doi: 10.3969/j.issn.1674-5671.2014.04.01 Abstract ( 268 )   PDF (406KB) ( 686 )   Save Related Articles | Metrics

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Research progress of radiotherapy and chemotherapy for esophageal carcinoma YOU Jin,ZHU Guang-ying 2014, 6 (4):  327-331.  doi: 10.3969/j.issn.1674-5671.2014.04.02 Abstract ( 270 )   PDF (408KB) ( 692 )   Save Related Articles | Metrics

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Radiation therapy for colorectal cancer :from the perspective of subtraction WU Jun-xin,CHEN Hui-qin 2014, 6 (4):  332-335.  doi: 10.3969/j.issn.1674-5671.2014.04.03 Abstract ( 303 )   PDF (366KB) ( 607 )   Save Related Articles | Metrics

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Screening for proteins differentially expressed between nasopharyngeal carcinoma xenografts sensitive or resistant to 10 Gy X-irradiation using a combination of iTRAQand OrbiTrap MS/MS MA Hui,LU Xiao-di,QU Song,ZHAO Wei,LI Lin, GUO Ya,HUANG Yuan-jiao,FU Qiang,CEN Wei-jian,WANG Jin-zi,ZHU Xiao-dong 2014, 6 (4):  336-341.  doi: 10.3969/j.issn.1674-5671.2014.04.04 Abstract ( 295 )   PDF (517KB) ( 1050 )   Save Objective To detect proteins differentially expressed between radiosensitive and radioresistant nasopharyngeal carcinoma xenografts exposed to 10 Gy X-irradiation in an effort to identify proteins related to radiotherapy resistance.Methods Xenograft tumors of radiosensitive CNE-2 cells or radioresistant CNE-2R cells were established in nude mice. Proteins were extracted，digested into peptides，and labeled using the iTRAQ kit. Peptide mixtures were separated by 2D-HPLC，then analyzed by LTQ-Orbitrap massspectrometry. Differentially expressed proteins were classified according to gene ontology analysis. Results A total of 61 differentially expressed proteins were identified，of which 17 were up-regulated and 44 were down-regulated. GO analysis showed these proteins to be involved in key biological processes such as cell cycle regulation，catabolism，signaling pathway regulation，response to pressure tress and redox reactions. Conclusion MudPIT analysis identified the following 7 proteins that may help render nasopharyngeal tumor issue radioresistant: Annexin2，PDIA3，Ubiquitin，CKAP4，HSPA8，14-3-3δ/ζ，and NLRC4. Related Articles | Metrics

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Construction of a plasmid expressing small hairpin RNA against human telomerase reverse transcriptase and functional analysis in the CaSki cervical cancer line ZHAO Hong-ke,MO Ling-zhao 2014, 6 (4):  342-347.  doi: 10.3969/j.issn.1674-5671.2014.04.05 Abstract ( 312 )   PDF (656KB) ( 632 )   Save Objective To construct a plasmid expressing a small hairpin RNA（shRNA）to knockdown expression of human telomerase reverse transcriptase （hTERT），and to observe its effects on hTERT expression in caski cervical cancer cells. Methods Designer   3.0 software （Genepharma）was used to design an shRNA targeting hTERT. The shRNA was cloned into the pGLV3/Hi/GFP+Puro   vector and confirmed by sequencing. Cells were divided into blank control group，negative control group and hTERT interference   group. The resulting plasmid was transfected into caski cervical cancer cells and expression of the GFP reporter was analyzed. In   addition，expression of chromosomal hTERT mRNA was quantified using RT-PCR. Cell proliferation in the presence and absence of shRNA was measured using the cck-8 assay.Results A plasmid expressing an shRNA targeting hTERT was constructed and used to generate recombinant lentivirus. Lentiviral infection of caski cells led to lower expression of hTERT mRNA and slower cell   proliferation than in controls. Conclusion A plasmid expressing shRNA targeting hTERT can effectively knockdown endogenous   hTERT expression. This reagent may prove useful for understanding the role of telomerase in the pathogenesis of cervical cancer. Related Articles | Metrics

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Effects of nigericin on Wnt/β-catenin signaling in DU145 prostate cancer cells YOU Zhe-bin,JIN Run-sen 2014, 6 (4):  348-352.  doi: 10.3969/j.issn.1674-5671.2014.04.06 Abstract ( 303 )   PDF (538KB) ( 586 )   Save Objective To evaluate the effect of nigericin on DU145 prostatecancer cells and explore its influence on Wnt/β-catenin signaling. Methods The human prostate cancer cell line DU145 was treated with 5，10 or 20 μmol/L nigericin or with DMSO as a control. Cultures were assessed for viability using the Cell Counting Kit-8，and for migration and invasion ability using transwell assays. The expression of β-catenin and its downstream target genes was analyzed using Western blotting， immunofluorescence and TOP/FOP-flash reporter assays. Results Nigericin showed significant toxicity towards DU145 cells and markedly inhibited their migratory and invasive abilities. The drug also reduced the expression of β-catenin，suppressing the Wnt/β-catenin pathway and down-regulating downstream target genes. Conclusion These findings suggest that nigericin significantly inhibits invasion and metastasis of DU145 prostate cancer cells in vitro， and that the drug likely exerts these effects by suppressing Wnt/β-catenin signaling. Related Articles | Metrics

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Effect of Panax Notoginsenosides on renal proteins differentially expressed in rats experi-encing cisplatin-induced nephrotoxicity YANG Yu-fang,LIU Hua-gang,XI Jia-xi,LIU Xin-wen 2014, 6 (4):  353-358.  doi: 10.3969/j.issn.1674-5671.2014.04.07 Abstract ( 262 )   PDF (422KB) ( 444 )   Save Objective To understand the effects of Panax Notoginsenosides（PNS） on renal proteins differentially expressed in rats experiencing cisplatin-induced nephrotoxicity. Methods Rats were randomly divided into three groups and treated with saline，cisplatin alone or cisplatin with PNS. At 10 days after treatment，renal tissue was examined for pathology，and levels of serum BUN，Scr，and urine NAG were determined. Renal proteins for which expression levels changed in response to cisplatin treatment were screened using SELDI-TOF-MS and identified using MALDI-TOF-MS/MS and Western blotting. Results We successfully created a rat model of cisplatin-induced nephrotoxicity，and PNS reduced the drug-induced damage. Mass spectrometry identified 20 renal proteins differentially expressed between the saline and cisplatin groups， as well as 18 proteins differentially expressed between the cisplatin and cisplatin+PNS groups. Of these proteins，6 were up-or down-regulated by cisplatin treatment，and PNS returned their expression close to the levels in the saline group. One of these proteins（m/z 10815.42） was identified as mitochondrial heat shock protein， while another （m/z 16021.67） was identified as hemoglobin subunit beta-1 and beta-2. Conclusions These differentially expressed renal proteins may mediate cisplatin-induced nephrotoxicity as well as the protective effects of PNS. Further studies should verify these leads and examine the cellular pathways involved. Related Articles | Metrics

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Identifying deregulated networks of protein-protein interactions through proteomics analysis of human hepatocellular carcinoma tissue XIAO Zhong-hua,MIAO Jia-wei,HUANG Hai-bing 2014, 6 (4):  359-363.  doi: 10.3969/j.issn.1674-5671.2014.04.08 Abstract ( 239 )   PDF (357KB) ( 619 )   Save Objective To analyze human hepatocellular carcinoma tissue using proteomics to identify possible deregulated networks of protein-protein interactions. Methods PubMed was searched using keywords “hepatocellular carcinoma” and “proteomics” to identify reports of proteins that are deregulated in the cancerous state. Then the proteins were analyzed for interactions using PRINCESS. Results Nine protein-protein interactions involving 8 proteins were assigned confidence scores >2.0：APEX1 interacts directly with ILF2，PRDX3，ANP32A，and MATR3 and indirectly with IQGAP2. SULT1A1 interacts directly with PDIA6. Conclusion APEX1，ILF2，PRDX3，ANP32A，MATR3，and IQGAP2 may participate in a single network associated with onset and/or progression of hepatocellular carcinoma. Related Articles | Metrics

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Comparative efficacy of hepatic resection or transarterial chemoembolization for treating intermediate-and advanced-stage hepatocellular carcinoma ZHONG Jian-hong,KE Yang,WANG Yan-yan,YOU Xue-mei,LI Hang,LI Le-qun,MA Liang 2014, 6 (4):  363-370.  doi: 10.3969/j.issn.1674-5671.2014.04.09 Abstract ( 337 )   PDF (389KB) ( 572 )   Save Objeclive Different official guidelines recommend different treatment modalities for intermediate-and advanced-stage hepatocellular carcinoma （HCC）. The aim of this study was to explore the safety and efficacy of hepatic resection（HR） in these patients. Methods Between 2000 and 2007，a consecutive sample of 1 259 patients with intermediate and advanced stage HCC who underwent HR （n=908） or transarterial chemoembolization （TACE，n=351） were included. Propensity score-matched patients were analyzed to adjust for any baseline differences. Results The 90-day mortality rates in the HR and TACE groups were 3.1% and 2.8% （P=0.827）. The morbidity rate was significantly higher in the HR group （26.8%） than in the TACE group （18.5%，P=0.005）. HR provided a survival benefit over TACE at 1，3，and 5 years （88% vs 81%，62% vs 33%， and 39% vs 16%，respectively；P<0.001）. Propensity scoring and subgroup analyses based on tumor size，tumor number， presence or absence of macrovascular invasion，and portal hypertension （PHT） also showed that HR was associated with better long-term survival than TACE. Multivariate analyses revealed that alpha-fetoprotein ≥400 ng/ml， diabetes mellitus， macrovascular invasion，and PHT were independent predictors of poor prognosis. Conclusions For patients with HCC and Child-Pugh A liver function， the presence of a solitary large tumor，multin-odular tumors， macrovascular invasion，or PHT are not contraindications for HR. For these patients，HR provides better survival than TACE. Related Articles | Metrics

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Effects of different Helicobacter pylori loads on proliferation，apoptosis and FAF1 mRNA expression in gastric cancer cells FENG Jie,LIU Ai-qun,YUAN Yan-ling,GE Lian-ying 2014, 6 (4):  371-375.  doi: 10.3969/j.issn.1674-5671.2014.04.10 Abstract ( 352 )   PDF (538KB) ( 493 )   Save Objective To explore the molecular involvement of Helicobacter pylori in gastric cancer by examining the effects of different H. pylori loads on proliferation，apoptosis and FAF1 mRNA expression in gastric cancer cells. Method Human gastric carcinoma HGC-27 cells were co-cultured with different amounts of standard H. pylori strain NCTC11637；multiplicities of infection （MOIs，bacteria/cells） were 0，1，50，100 and 200. H. pylori was identified based on Gram staining and biochemical tests. Cancer cell proliferation was measured using the thiazolyl blue（MTT） assay after 0，12，24，36 and 48 h of co-culture. After 24 h co-culture，apoptosis in the cancer cells was measured using flow cytometry，and FAF1 mRNA expression was quantitated using fluorescent real-time quantitative RT-PCR. Expression of the β-actin gene was used as a reference for normalizing FAF1 mRNA expression levels. Results After 12 h co-culture，cancer cell proliferation at MOI 50 was significantly greater than at MOI 0（P<0.05），whereas proliferation at other MOIs was lower than at MOI 0（P<0.05）. After co-culture for 24，36 and 48 h，cancer cell proliferation at all MOIs was significantly lower than at MOI 0（P<0.05）. After 24 h co-culture，apoptosis levels and relative  FAF1 mRNA expression were similar at MOIs 0，1，and 50 （P＞0.05）. In contrast，apoptosis levels were significantly higher and relative  FAF1 mRNA expression significantly lower at MOIs 100 and 200 than at MOI 0（P<0.05）. Conclusion H. pylori infection can suppress proliferation， promote apoptosis and down-regulate FAF1 mRNA expression in gastric cancer cells-regulation of FAF1 mRNA by H. pylori may lead to gastric cancer. Related Articles | Metrics

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Pleural infusion chemotherapy for lung cancer patients with pleural effusion：clinical significance of serum Cyfra21-1，CA125，CEA，and NSE LI Fang-hua 2014, 6 (4):  376-379.  doi: 10.3969/j.issn.1674-5671.2014.04.11 Abstract ( 521 )   PDF (304KB) ( 587 )   Save Objective To observe the effects of thoracic cavity perfusion chemotherapy on cytokeratin 19 fragment （Cyfra21-1），CA125，carcinoembryonic antigen（CEA），and neuron-specific enolase（NSE） in patients with non-small cell lung cancer and malignant pleural effusion. Methods A total of 70 patients with non-small cell lung cancer and malignant pleural effusion were subjected to closed drainage of the thoracic cavity using a central venous catheter，and cisplatin reperfusion of the thoracic cavity. Chemiluminescence immunoassay was performed before and after these procedures in order to monitor levels of serum Cyfra21-1，CA125，CEA，and NSE. Curative efficacy was measured using WHO standards for purulent cancer exudate，and adverse reactions were observed. Results The total efficacy rate was 75.71%，and the disease control rate was 85.71%. Levels of CA125，serum Cyfra21-1，CEA，and NSE were lower after pleural reperfusion than before（P<0.001）. The main adverse reactions were gastrointestinal reactions and bone marrow suppression （degree I-II events）；no damage to liver or kidneys was observed. Conclusion Pleural infusion chemotherapy in the treatment of non-small cell lung cancer pleural effusion can effectively control pleural effusion and decrease the levels of serum Cyfra21-1，CA125， CEA，and NSE. This therapy may also help clinicians predict sensitivity to platinum-based chemotherapy in patients receiving systemic treatment. Related Articles | Metrics

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Hepatic expression of human fibroleukin in patients with liver cancer or hepatitis MENG Meng-lan,YU Yan-hong,HUANG Li-yi,WU Ji-zhou 2014, 6 (4):  380-383.  doi: 10.3969/j.issn.1674-5671.2014.04.12 Abstract ( 252 )   PDF (375KB) ( 393 )   Save Objective To explore the expression of human fibroleukin（hFgl 2），also known as human fibrinogen-like protein 2 prothrombinase，in the livers of patients with liver cancer or chronic hepatitis B virus（HBV） infection，and to examine possible correlations of this expression with severity of HBV infection and serum biochemistry indicators. Methods Liver tissues were obtained from 35 patients with chronic HBV infection（14 mild， 13 moderate，8 severe） and 21 patients with liver cancer. Expression of hFgl 2 was detected using immunohistochemistry，quantified using MIAS medical imaging software and compared among patients with liver cancer or the three severities of viral infection. Results Expression of hFgl 2 was significantly higher in patients with severe chronic HBV infection or liver cancer than in patients with mild or moderate infection. Among chronically infected patients， hFgl 2 expression positively correlated with serum ALT and AST；among patients with liver cancer， hFgl 2 did not correlate with serum ALT，AST，TBIL or PT. Conclusion The protein hFgl 2 is up-regulated in severe chronic HBV infection and in liver cancer. However， the expression level correlated with serum aminotransferase in chronically HBV-infected patients，but not in patients with liver cancer. This suggests that hFgl 2 may play different roles in liver cancer and chronic HBV infection. Related Articles | Metrics

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Expression and clinical significance of osteopontin in esophageal squamous carcinoma Ren Min-zhu 2014, 6 (4):  384-387.  doi: 10.3969/j.issn.1674-5671.2014.04.13 Abstract ( 233 )   PDF (451KB) ( 534 )   Save Objective To analyze the expression and clinical significance of osteopontin（OPN）in esophageal carcinoma. Methods Immunohistochemistry was used to detect the expression of OPN in normal esophageal tissue and in 120 specimens of esophageal carcinoma tumor tissue and tumor-adjacent tissue showing atypical hyperplasia. Results OPN expression density was lowest in normal esophageal tissues （0），higher in tumor-adjacent tissues showing atypical hyperplasia（34.78%） and highest in esophageal carcinoma tissues（76.67%）. Expression of OPN protein correlated with tumor TNM stage，lymph node metastasis and infiltrative depth of esophageal squamous cell carcinoma（P<0.05）. The five-year survival rate was significantly higher in patients negative for OPN expression than in those positive for OPN expression（28.6% vs 10.7%，P<0.05）. Conclusion Studying OPN expression may generate insights into the pathogenesis， invasion and metastasis of esophageal carcinoma，and it may provide tools for predicting the prognosis of patients with the disease. Related Articles | Metrics

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Comparing the therapeutic efficacy of surgical resection and radio frequency ablation to treat metastatic liver cancer within 5 cm WEI Xiao-bo,WU Fei-xiang,Chen Jian-si,YUAN Wei-ping,LIU Jian-yong,XIANG Bang-de,WU Guo-bin,HUANG Shan 2014, 6 (4):  388-392.  doi: 10.3969/j.issn.1674-5671.2014.04.14 Abstract ( 390 )   PDF (352KB) ( 538 )   Save Objective To compare the therapeutic efficacy of Surgical Resection （RES） and Radio Frequency Ablation （RFA） for treating patients baring metastatic liver cancer within 5 cm， and analysis the prognostic factors of RES group. Methods Records were retrospectively reviewed for 88 patients with metastatic liver cancer，who has been admitted by the Affiliated Tumor Hospital of Guangxi Medical University from January 2008 to December 2012. 49 cases received Surgical Resection treatment（RES group） and 33 cases were underwent Radio Frequency Ablation treatment（RFA group）. The potentially prognostic factors of RES group were examined by univariate and multivariate analyses. Results ⑴The 1，2 and 3-year survival rates were 81.6%，68.5% and 50.4% in RES group， respectively，and 84.8%，69.4% and 50.7% in RFA group， respectively，then there were no statistical significance between these two groups（P>0.05）. The 1，2 and 3-year rates of recurrence and metastasis in RES group were 28.8%，54.2% and 68.3%，and showed in 34.9%，50.5% and 69.2% in RFA group（P>0.05）.　⑵The univariate analysis showed that the number of the liver metastases，degree of differentiation of the primary tumor and lymph node metastasis were associated with the prognosis of the patients（P<0.05）. The Cox multi-variate analysis showed lymph node metastasis is an independent prognostic factor affecting the patients of metastatic liver cancer（P<0.05）. Conclusion ⑴ The short-term therapeutic effect in metastatic liver cancer patients， whose lesion is up to 5　cm， seems not to differ in RFA and RES. But from the point of the treatment，RFA，which has a lower trauma than RES，might be a prior selection for metastatic liver cancer patients.　⑵ the number of the liver metastases，the degree of differentiation of the primary tumor and lymph node metastasis are associated with the prognosis of the patients. Lymph node metastasis is an independent prognostic factor affecting the patients of metastatic liver cancer. Related Articles | Metrics

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Determination of thyroid volume by MRI and clinical applications FANG Xian-liu,SU Dan-ke,JIN Guan-qiao,KANG Wei,LIU Yu,LI Qiang,HUANG Wei-li 2014, 6 (4):  392-395.  doi: 10.3969/j.issn.1674-5671.2014.04.15 Abstract ( 331 )   PDF (306KB) ( 527 )   Save Objective To investigate the value of MRI for measuring thyroid volume. Method Thyroids of 51 normal adults were scanned by MRI using T1WI-and T2WI-weighted imaging technology. Volumes were compared for the left and right thyroid lobes and for the two genders. Results Mean total thyroid volume was（14.297±5.44） cm3；mean volume was similar for the right lobe （7.395±2.858） cm3 and left lobe （6.902±2.656） cm3；P>0.05）. Total thyroid volume was significantly greater in men（n=26）than in women（n=25，P<0.05）. Comparing the left lobe with right lobe between men and women，the volume of bilateral lobe volume in men was significantly larger than those in women. The difference was statistically significant（P<0.05）. Conclusion MRI may allow noninvasive measurement of thyroid volume，and the results suggest the need to take into account gender differences in thyroid volume when making treatment decisions. Related Articles | Metrics

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Efficacy of transcatheter arterial chemoembolization combined with Gyro Knife in the treatment of primary liver cancer WANG Juan,LU Yao-zhen,HUANG Xian-shi 2014, 6 (4):  395-398.  doi: 10.3969/j.issn.1674-5671.2014.04.16 Abstract ( 235 )   PDF (334KB) ( 494 )   Save Objective To observe the effect of transcatheter arterial chemoembolization（TACE） combined with cobalt-60 gyro rotating radiation （Gyro Knife） in the treatment of primary liver cancer. Methods Retrospective analysis was performed of 71 patients with primary liver cancer who were treated at our hospital from June 2008 to January 2011 using either TACE alone（n=35） or TACE combined with Gyro Knife（n=36）． Response and survival rates were compared for the two treatments. Results Combination therapy gave a significantly higher response rate（77.8％） than TACE alone（54.3％，P<0.05）．Survival rates were significantly higher in the combination therapy group at various time points：97.2% vs 71.4% at 6 months，83.8% vs 51.4% at 12 months，52.8% vs 34.3% at 18 months，and 41.7% vs 28.6% at 24 months（all P<0.05）．Among the combination therapy group three patients experienced fever and four experienced hepatic toxicity when received gyro knife radiotherapy，no cases of radiation pneumonia or bone marrow suppression were observed in either group. Conclusion TACE combined with Gyro Knife therapy may be safe and effective for treating primary liver cancer. Related Articles | Metrics

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Efficacy of bronchial sleeve lobectomy (BSL) in treating central-type lung cancer PENG Guo-qing,XIAN Lei 2014, 6 (4):  399-402.  doi: 10.3969/j.issn.1674-5671.2014.04.17 Abstract ( 288 )   PDF (328KB) ( 470 )   Save Objective To explore the effectiveness of bronchial sleeve lobectomy（BSL）for treating central-type lung cancer. Methods Complications during and after surgery，quality of life and survival rates at 1，3 and 5 years were retrospectively analyzed in 79 patients with central lung cancer，comprising 37 who underwent bronchial sleeve lobectomy（BSL） and 42 who underwent pneumonectomy. Results Postsurgical complications were significantly less frequent in the BLS group（18.9%） than in the pneumonectomy group（40.5%；P＜0.05）. Although the two groups presented similar survival rates at 1 and 3 years，the BLS group showed a significantly higher 5-year survival rate（54.1% vs 42.9%；P＜0.01）. The BLS group also showed significantly lower rates of cancer recurrence（27.0% vs 35.7%；P＜0.0） and cancer metastasis（16.2% vs 42.9%；P＜0.01）. The BLS group scored significantly higher on quality of life（P＜0.05）. Conclusion BSL can reduce the complication rate and increase both quality of life and survival after surgery to treat central-type lung cancer. Related Articles | Metrics

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Clinical characteristics of skin cancer in the southern edge of Xinjiang desert region WEI Li-ming,HU Hua,DU Jiang-bo,ZHU Yong-an,WANG Xiao-qin,WANG Dong-li,XUE Jun-hai,FANG Hong-mei 2014, 6 (4):  403-404.  doi: 10.3969/j.issn.1674-5671.2014.04.18 Abstract ( 324 )   PDF (237KB) ( 420 )   Save Related Articles | Metrics

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Influence of intravenous patient-controlled analgesia on circulatory and respiratory systems when waking up from general anesthesia for cancer patients HUANG Ling,ZHANG Ai-gui,ZHANG Li-feng,MENG Li-ying,LI Yang,HUANG Bing,PAN Ling-hui 2014, 6 (4):  405-407.  doi: 10.3969/j.issn.1674-5671.2014.04.19 Abstract ( 260 )   PDF (294KB) ( 619 )   Save Related Articles | Metrics

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miR-221/miR-222 and tumor LIAO Li,FENG Shui-dong 2014, 6 (4):  408-411.  doi: 10.3969/j.issn.1674-5671.2014.04.19 Abstract ( 164 )   PDF (283KB) ( 417 )   Save Related Articles | Metrics

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The research progress of breast cancer screening method and model TANG Wei,YANG Hua-wei,LIU Jian-lun 2014, 6 (4):  411-414.  doi: 10.3969/j.issn.1674-5671.2014.04.21 Abstract ( 233 )   PDF (302KB) ( 812 )   Save Related Articles | Metrics

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Research progress in hepatoid adenocarcinoma of the stomach LUO Qing-wei,LIU Li-ming,TIAN Xiao-lin 2014, 6 (4):  414-417.  doi: 10.3969/j.issn.1674-5671.2014.04.22 Abstract ( 314 )   PDF (320KB) ( 646 )   Save Related Articles | Metrics

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Hepatic resection is safe and effective for patients with hepatocellular carcinoma and portal hypertension ZHONG Jian-hong 2014, 6 (4):  417-418.  doi: 10.3969/j.issn.1674-5671.2014.04.23 Abstract ( 253 )   PDF (225KB) ( 564 )   Save Related Articles | Metrics