Table of Content

25 June 2017, Volume 9 Issue 3 Previous Issue    Next Issue

Select

Identification of novel serum biomarkers for recurrent nasopharyngeal carcinoma using TMT-based quantitative proteomic analysis Meng Huiling,Zhu Xiaodong,Li Ling,Liang Zhongguo,Pan Xinbin,Zeng Fanyan,Qu Song 2017, 9 (3):  167-171.  doi: 10.3969/j.issn.1674-5671.2017.03.01 Abstract ( 341 )   PDF (518KB) ( 631 )   Save Objective To identify proteins differentially expressed between recurrent nasopharyngeal carcinoma（NPC） and non-recurrent NPC. Method Serum was collected from 20 patients with recurrent NPC and 20 patients with non-recurrent NPC. We used tandem mass tag labeling and high performance liquid chromatography fractionation，followed by liquid chromatography-tandem mass spectrometry to identify differentially expressed proteins. Candidate proteins were confirmed by ELISA in a separate set of serum samples comprising 40 patients with recurrent NPC and 41 with non-recurrent NPC. Results A total of 635 protein groups were identified in human serum，and expression levels of 413 proteins were quantified. In two independent tests with different subgroups of patients，41 proteins were found to be down-regulated and 18 to be up-regulated in recurrent disease. ELISA confirmed that A2M was down-regulated in 23 patients with recurrent disease compared to 30 with non-recurrent disease. （Other patients had A2M levels below the detection threshold of the ELISA）. Spearman analysis showed that A2M concentration did not correlate with clinical stage （r =-0.024，P=0.866） or age（r =-0.087，P=0.534）. Conclusion A2M expression may protect against risk of NPC recurrence and so may be a useful prognostic marker. Related Articles | Metrics

Select

Analysis of hepatitis B virus X gene integration and potential risk factors of integration in patients with hepatocellular carcinoma in the high-incidence area of Guangxi Wu Hanghang,Huang Tianren,Deng Wei,Fang Xiang,Ren Jingjing,Gan Yingyuan 2017, 9 (3):  172-176.  doi: 10.3969/j.issn.1674-5671.2017.03.02 Abstract ( 231 )   PDF (734KB) ( 377 )   Save Objective To analyze integration of hepatitis B virus（HBV） X gene into the genome of primary hepatocellular carcinoma （HCC） tumors and surrounding tissue，and to explore potential risk factors of such integration in patients from the HCC high-incidence area of Guangxi. Methods DNA was extracted from HCC tissue and adjacent non-tumor liver tissue of 30 patients with HBV-related HCC. Primers were designed to amplify the HBV X sequence and human Alu repeats by Alu-PCR，and PCR products were Sanger- sequenced. Results Among the 30 patients，18（60.00%） showed integration of the HBV X gene into HCC tumors，compared to 26 （86.67%） who showed X gene integration into adjacent liver tissue （χ2=5.445，P=0.020）. Incidence of X gene integration did not vary significantly with sex，age，HbeAg status，HBV DNA load，ALT or AST in HCC tissue or adjacent non-tumorous liver tissue（P>0.05）.Conclusions Incidence of HBV integration is higher in non-tumorous tissues than in tumorous tissues in patients with HBV-related HCC in Guangxi.HBV integration appears to occur in early stages of HCC development. HBV X gene integration does not appear to be influenced by sex，age，HBeAg status，HBV DNA load，ALT or AST. Related Articles | Metrics

Select

Identification of potential target genes and molecular mechanisms of miR-10b-5p in pancreatic cancer Jiang Mengtong,Tang Ruixue,Luo Dianzhong 2017, 9 (3):  177-181.  doi: 10.3969/j.issn.1674-5671.2017.03.03 Abstract ( 227 )   PDF (632KB) ( 359 )   Save Objective We aimed to explore target genes and molecular mechanisms potentially affected by miR?鄄10b?鄄5p in the devel?鄄opment of pancreatic cancer. Methods We predicted potential target genes affected by miR?鄄10b?鄄5p using the Gene Expression Omnibus database and other online prediction databases. Results A total of 586 candidate target genes were identified，and Gene Ontology enrichment analysis showed that many target genes are involved in protein transport. Pathway analysis using the Kyoto Encyclopedia of Genes and Genomes suggested that miR?鄄10b?鄄5p is involved in tumor?鄄associated pathways as well as specifically in pancreatic cancer?鄄related pathways. Network analysis using the Protein?鄄Protein Interaction tool suggested interactions among seven mutant genes （EGFR，RAC3，SMAD4，PIK3CA，CDK6，Bcl2L1，STAT3） in pancreatic cancer?鄄related pathways. Conclusions The microRNA miR?鄄10b?鄄5p may target genes and pathways involved in pancreatic cancer，potentially making it important in diagnosis and treatment of the disease. Related Articles | Metrics

Select

A case-control study of association between hepatitis B virus S gene mutation and primary liver cancer Gan Yingyuan,Deng Wei,Huang Tianren,Wu Hanghang,Huang Yueying,Zhang Yuli 2017, 9 (3):  181-185.  doi: 10.3969/j.issn.1674-5671.2017.03.04 Abstract ( 294 )   PDF (680KB) ( 277 )   Save Objective To study potential associations between mutations in hepatitis B virus （HBV） S gene and risk of primary liver cancer（PLC） in FuSui county of the Guangxi Zhuang Autonomous Region. Methods A total of 30 new cases of PLC from individuals at high PLC risk in FuSui（based on HBsAg positivity lasting from 2011 to 2015） were matched with 30 controls without cancer. The S gene was amplified from DNA in serum by nested PCR and sequenced directly. Results were analyzed by chi-squared test，Fisher's exact test and conditional logistic regression. Results Frequencies of the mutations T140A，T140C，T300C and G620A differed signifi-cantly between cases and controls（P<0.05）. Logistic regression linked T140C to significantly lower risk of PLC（OR 0.484，95% CI ：0.239-0.994） and T300C with significantly higher risk of PLC（OR 2.911，95% CI ：1.110-7.629）. Conclusion T140C and T300C are independent predictors of PLC，with T140C an apparent protective factor and T300C an apparent risk factor. Related Articles | Metrics

Select

Expression of CD90 and CD44 in human hepatoma tissues Zhou Silian,Kuang Zhipeng 2017, 9 (3):  185-189.  doi: 10.3969/j.issn.1674-5671.2017.03.05 Abstract ( 373 )   PDF (4061KB) ( 301 )   Save Objective To investigate differences in CD90 and CD44 expression between human hepatoma and distal liver. Method Expression of CD90 and CD44 was measured using quantitative real-time PCR in human hepatoma and distal liver tissue，and the expression of both proteins was detected by immunohistochemistry. Results Expression of CD90 and CD44 mRNA was significantly higher in human hepatoma than in distal liver （P<0.05）. CD90 and CD44 proteins localized to the membrane and cytoplasm of cancer cells. In human hepatoma，the CD90-positive rate was 82.5% （33/40） and the CD44-positive rate was 77.5%（31/40）；the correspond-ing values in distal liver tissue were significantly lower：17.5%（7/40） and 12.5%（5/40）（P<0.05）. Conclusion  Human liver cancer tissue shows high expression of CD90 and CD44，while distal liver tissue shows low expression. CD90 and CD44 may correlate signif-icantly with the incidence and development of liver cancer. Related Articles | Metrics

Select

Expression of Survivin,NF-κB and VEGFR-2 and their relationship with Apatinib therapy in small-cell carcinoma of esophagus   Cheng Zhifu,Meng Lingxin,Ding Zhaojun,Meng Qin,Wang Chuanyan,Zhang Guifang,Li Bingcheng 2017, 9 (3):  190-195.  doi: 10.3969/j.issn.1674-5671.2017.03.06 Abstract ( 243 )   PDF (499KB) ( 356 )   Save Objective To investigate the expression of Survivin，NF-κB，and VEGFR-2 as well as their relationships with treatment effects of Apatinib and with microvascular density and micro-lymphatic vessel density in small-cell carcinoma of esophagus. Methods Immunohistochemistry was used to assess expression of Survivin，NF-κB and VEGFR-2 as well as microvascular density and micro-lymphatic vessel density in 36 cases of small-cell carcinoma of the esophagus. The results were compared with Apatinib treatment efficacy. Results Rates of positive expression were 22.22% for Survivin，66.67% for NF-κB and 80.56% for VEGFR-2. Lymphatic metastasis did not correlate with expression of Survivin，micro-lymphatic vessel density or tumor invasion depth. Lymphatic metastasis did correlate with expression of VEGFR-2，NF-κB and microvascular density，but not with tumor invasion depth. Microvascular density was higher in samples positive for NF-κB expression than in negative samples. Treatment effects of Apatinib were better in patients positive for NF-κB and VEGFR-2 expression than in expression-negative patients；treatment effects，however，did not vary with Survivin expression status. Conclusion Tumor lymphatic metastasis correlated significantly with VEGFR-2，NF-κB and microvascular density. NF-κB and VEGFR-2 may play an important role in small-cell carcinoma and may be useful biomarkers in Apatinib therapy. Related Articles | Metrics

Select

Influence of malignant anemia on anesthetic efficacy of sevoflurane based-inhalational anesthesia and propofol intravenous anesthesia Li Zhenbin,Lin Fei,Ge Wanyun,Dai Huijun,Pan Linghui 2017, 9 (3):  195-200.  doi: 10.3969/j.issn.1674-5671.2017.03.07 Abstract ( 260 )   PDF (427KB) ( 376 )   Save Objective To compare the effects of different severities of malignant anemia on anesthetic efficacy of sevoflurane based-inhalational anesthesia and propofol intravenous anesthesia. Methods A total of 120 patients undergoing elective laparoscopic surgery during genneral anesthesia at our hospital were enrolled in this prospective study.Patients were divided into three groups based on preoperative hemoglobin level：normal hemoglobin （n=40，Hb≧120 g/L），mild anemia （n=40，90≤Hb<120 g/L），and moderate anemia （n=40，60≤Hb<90 g/L）. Patients were also divided into one group that received sevoflurane inhalation anesthesia（n=20） and one group that received intravenous anesthesia with propofol （n=20）. Pulse oxygen saturation （SpO2），mean blood pressure （MBP），heart rate （HR） and bispectral index （BIS） were recorded before induction （T1），before intubation （T2），at intubation （T3），at pneumoperitoneum （T4），at extubation （T5） and 2 h after operation （T6）. Data were recorded on pneumoperitoneum pressure and duration，hemorrhage volume，transfusion volume of fluid and blood，vasoactive therapy，propofol or sevoflurane dosage，time to open eyes，time to intubation，incidence of postoperative agitation，and incidence of postoperative nausea and vomiting. Serum levels of cortisol and 5-serotonin （5-HT） were assessed using enzyme-linked immunosorbent assay. Results Dosage of sevoflurane was similar across the normal hemoglobin，mild anemia and moderate anemia subgroups among patients receiving sevoflurane.In contrast，propofol dosage differed significantly between the normal hemoglobin and moderate anemia subgroups. Patients receiving sevoflurane showed significantly shorter time to eye opening than those receiving propofol，but they also showed significantly higher incidence of postoperative nausea，vomiting and dysphoria.Cortisol and 5-HT concentrations were significantly higher at T3 and T5 than at T1，while levels were significantly lower at T2 than at T1. Conclusions Sevoflurane inhalation anesthesia was associated with faster revival but also higher incidence of postoperative nausea，vomiting and dysphoria than intravenous anesthesia.Moderate anemia was associated with lower propofol dosage and volatile hemodynamics. Related Articles | Metrics

Select

Clinical significance of Caveolin-1 expression in endometrial carcinoma Han Yanyan,Zhu Junbao,Li Shuhua 2017, 9 (3):  201-204.  doi: 10.3969/j.issn.1674-5671.2017.03.08 Abstract ( 297 )   PDF (503KB) ( 316 )   Save Objective To evaluate the expression of Caveolin-1 in endometrial carcinoma and investigate its association with pathological features. Methods Caveolin-1 expression was determined by immunohistochemistry and Western blot using rabbit polyclonal antibody in 45 cases of endometrial carcinoma，35 cases of hyperplastic endometrium，and 35 cases of normal endometrium.Caveolin-1 mRNA levels in endometrial carcinoma tissue were determined using real-time PCR. Results Immunohistochemistry and Western blot showed different rates of positive expression of Caveolin-1 in the different types of endometrium. Caveolin-1 expression tended to increase with degree of malignancy（P<0.05）.Among cases with endometrial carcinoma，Caveolin-1 expression was much higher in patients with lymph node metastasis （P=0.001），and it was higher in stages Ⅱ and Ⅲ+Ⅳ than in stage （P=0.002，P=0.040）. Caveolin-1 expression was much higher in moderate and low-grade endometrial carcinoma than in high-grade endometrial carcinoma （P＜0.001，P=0.020）. Conclusions Caveolin-1 expression is higher in endometrial carcinoma than in hyperplastic or normal endometrium，and it correlates significantly with tumor stage and lymph node metastasis.Caveolin-1 may play an important role in endometrial carcinoma progression. Related Articles | Metrics

Select

Whole-brain radiotherapy involving a simultaneous integrated boost to brain metastases using volumetric modulated arc therapy：a dosimetric study Wang Zhongfei,Tan Lina,Sun Xiaohuan,Xiao Feng 2017, 9 (3):  205-209.  doi: 10.3969/j.issn.1674-5671.2017.03.09 Abstract ( 251 )   PDF (503KB) ( 380 )   Save Objective To perform a dosimetry study of the RapidArc technique for whole-brain radiotherapy involving simultaneous integrated boost （SIB） to brain metastases （BM） using volumetric modulated arc therapy （VMAT）. Methods Ten patients diagnosed with brain metastases were randomly selected and two treatment plans were designed for each patient. The RA plan involved whole-brain VMAT （30 Gy） and SIB to BM （45 Gy） delivered in 10 fractions. The Sum plan involved whole-brain 3DCRT （30 Gy） and SIB with VMAT to BM（15 Gy） delivered in 5 fractions. Results were compared using dose-volume histogram analysis. Results Both plans met the clinical requirements.Target conformity was better for the RA plan （0.99±0.00） than for the Sum plan（0.77±0.05； P<0.001）. V35 and V40 to brain were significantly lower in the RA plan than in the Sum plan（ P<0.05）. Average monitor units were 12% smaller with the RA plan than with the Sum plan. Conclusions The RA plan was similar to the Sum plan or better in terms of target dose distribution，monitor units and irradiation of some organs at risk，especially the normal brain tissue beside the metastases. These results suggest that whole-brain radiotherapy with SIB to BM using VMAT is superior for treating brain metastases. Related Articles | Metrics

Select

Primary testicular diffuse large B-cell lymphoma：series of 9 cases Mo Qinli,Peng Zhigang,Ma Jie,Yao Yibin,Tang Zhongyuan,Wu Zeguang 2017, 9 (3):  209-213.  doi: 10.3969/j.issn.1674-5671.2017.03.10 Abstract ( 496 )   PDF (357KB) ( 434 )   Save Objective To analyze the clinical characteristics，outcomes and treatment strategies of a case series of patients with primary testicular diffuse large B-cell lymphoma（PTDLBCL）. Methods Various clinical parameters were analyzed in 9 patients with PTDLBCL diagnosed from January 2013 to September 2016 in our hospital. Results Median age at diagnosis was 64 years （range，50-72）. Cases involved mainly the right testicle，and cells of origin were  non-GCB subtype（7 patients） or GCB subtype（2 patients）.Median rate of Ki-67 expression was 80%.One patient underwent surgery only，while seven had surgery followed by chemotherapy. Six patients showed complete response；two，partial response；and one，progression.Six patients were alive，corresponding to follow-up ranging from 5 to 34 months. Conclusion PTDLBCL has high recurrence risk. We suggest first-line treatment involving 6-8 cycles of anthracycline-based chemotherapy and rituximab treatment，central nervous system prophylaxis and contralateral testicular radiation after surgery. Related Articles | Metrics

Select

Value of 18F-FDG PET/CT for delineating the target area in cervical cancer Li Jing,Song Honglin,Pang Yebin,He Qing 2017, 9 (3):  214-216.  doi: 10.3969/j.issn.1674-5671.2017.03.11 Abstract ( 293 )   PDF (357KB) ( 323 )   Save Objective This study explored the value of 18F-FDG PET/CT for delineating the target area in cervical cancer. Methods A total of 33 patients with pathologically proven cervical squamous cell carcinoma in stage IIIB from March 2015 to October 2016 were enrolled. Based on the CT and PET/CT fusion images，3 experienced radiologists outlined the primary tumor target volume （gross target volume，GTV）and compared the differences between the target group and the group. Results Three physicians estimated significantly different GTVs based on pure CT and fusion images （P<0.001）. The LSD-t test showed a significant difference between different groups in GTVCT（F=4.28，P<0.001） but not GTVPET-CT（F=0.21，P=0.81）. Variation in tumor target volume decreased when PET/CT image delineation was used（7.75 cm3 vs 24.50 cm3）. Conclusions PET/CT fusion images can improve the accuracy of target delineation and reduce inter-observer variability. Related Articles | Metrics

Select

Analysis of malignant tumor death from 2010 to 2015 in the Daiyue district of Tai 'an,China An Xinggui,Xuan Lili,Zhang Linlin 2017, 9 (3):  217-221.  doi: 10.3969/j.issn.1674-5671.2017.03.11 Abstract ( 155 )   PDF (368KB) ( 312 )   Save Objective To analyse data on malignant tumor deaths in the Daiyue District of Tai'an from 2010 to 2015 in order to guide plan prevention and control interventions. Methods Crude and standardized mortality rates as well as annual percentage changes in mortality were calculated according to year，age，sex and cause of death using data on malignancy-related deaths in the Daiyue District of Tai'an from 2010 to 2015. Results Malignant neoplasms were associated with a crude mortality rate of 171.58 per 10 million，a China standardization mortality rate of 167.80 per 10 million，and a world standardization rate of 140.82 per 10 million.The annual trend in malignancy deaths was statistically significant（P<0.01）. Mortality rate increased with age for individuals older than 35，and overall mortality rate was higher in males than in females（χ2=222.15，P<0.001）. Lung cancer accounted for 38.5% of all cases，while gastric cancer，liver cancer，esophageal cancer，colon and rectal and anal cancer and other digestive system tumors accounted for 53.7% of cases. The top 10 causes of death were lung cancer，stomach cancer，liver cancer，esophageal cancer，colorectal and anal cancer，pancreatic cancer，breast cancer，leukemia，lymphoma，multiple myeloma，and bladder cancer. Conclusion The annual trend in malignancy deaths remain to be seen. Prevention and  control of  digestive system tumors should be controlled. Malignant tumors should be diagnosed and treated as early as possible in high-risk groups. Related Articles | Metrics

Select

Therapeutic effect of the combination of Baofukangshuang and thymosins in patients with high-risk HPV infection Jiang Qiumin,Yang Jinling 2017, 9 (3):  221-223.  doi: 10.3969/j.issn.1674-5671.2017.03.13 Abstract ( 316 )   PDF (362KB) ( 434 )   Save Related Articles | Metrics

Select

The outcomes of single-port laparoscopic surgery for rectal cancer Gai Lei,Zhang Xiaonong,Zhang Jinling,Li Mingjun 2017, 9 (3):  224-226.  doi: 10.3969/j.issn.1674-5671.2017.03.14 Abstract ( 213 )   PDF (428KB) ( 359 )   Save Related Articles | Metrics

Select

Misdiagnosis of cervical microglandular hyperplasia：a case report and literature review Li Haimei, Yu Jinxia, Yang Haijun 2017, 9 (3):  227-229.  doi: 10.3969/j.issn.1674-5671.2017.03.15 Abstract ( 278 )   PDF (363KB) ( 362 )   PDF（mobile） (428KB) ( 9 )   Save Related Articles | Metrics

Select

Regulation of the oxidative response to drug cancer drug resistance: recent progress Cen Juan,Liu Fangfang,Zhang Feng 2017, 9 (3):  229-232.  doi: 10.3969/j.issn.1674-5671.2017.03.16 Abstract ( 205 )   PDF (375KB) ( 417 )   Save Related Articles | Metrics

Select

Clinical value of circulating tumor cells in esophageal cancer Zhu Cheng,Li Lian 2017, 9 (3):  233-235.  doi: 10.3969/j.issn.1674-5671.2017.03.17 Abstract ( 203 )   PDF (332KB) ( 540 )   Save Related Articles | Metrics

Select

The enhanced recovery in hepatic surgery：controversies and applications Ma Liang,Su Qibin,Wang Yanyan,Zhu Shaoliang,Zhao Xinhua,You Xuemei,Xiang Bangde,Li Lequn 2017, 9 (3):  236-240.  doi: 10.3969/j.issn.1674-5671.2017.03.18 Abstract ( 248 )   PDF (365KB) ( 608 )   Save Related Articles | Metrics

Select

Dual energy CT: clinical application in the diagnosis of hepatocellular carcinoma Lai Shaolv,Luo Ningbin 2017, 9 (3):  241-243.  doi: 10.3969/j.issn.1674-5671.2017.03.19 Abstract ( 255 )   PDF (312KB) ( 354 )   Save Related Articles | Metrics

Select

Progress in hospital-acquired infections in cancer patients Lin Zhen 2017, 9 (3):  244-246.  doi: 10.3969/j.issn.1674-5671.2017.03.20 Abstract ( 186 )   PDF (335KB) ( 281 )   Save Related Articles | Metrics