Table of Content

25 June 2019, Volume 11 Issue 3 Previous Issue    Next Issue

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Expert consensus on the clinical application of nasopharyngeal carcinoma biomarkers Expert Committee of Nasopharyngeal Cancer Biomarker, Tumor Biomarker Committee of China Anti-Cancer Association 2019, 11 (3):  183-193.  doi: 10.3969/j.issn.1674-5671.2019.03.01 Abstract ( 560 )   PDF (2015KB) ( 844 )   Save Related Articles | Metrics

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Application of sentinel lymph node detection technique in early cervical cancer LIU Qing, LIU Kaijiang 2019, 11 (3):  194-198.  doi: 10.3969/j.issn.1674-5671.2019.03.02 Abstract ( 383 )   PDF (1241KB) ( 455 )   Save Related Articles | Metrics

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Advances in postoperative adjuvant therapy for early cervical cancer KANG Shan 2019, 11 (3):  199-203.  doi: 10.3969/j.issn.1674-5671.2019.03.03 Abstract ( 298 )   PDF (1560KB) ( 449 )   Save Related Articles | Metrics

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Thinking of laparoscopic surgery for early cervical cancer LU Anwei, ZHOU Li 2019, 11 (3):  204-207.  doi: 10.3969/j.issn.1674-5671.2019.03.04 Abstract ( 206 )   PDF (2154KB) ( 295 )   Save Related Articles | Metrics

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The value of laparoendoscopoc single-site surgery in the surgical treatment of cervical cancer MIN Ling, ZHENG Ying 2019, 11 (3):  208-211.  doi: 10.3969/j.issn.1674-5671.2019.03.05 Abstract ( 330 )   PDF (1738KB) ( 303 )   Save Related Articles | Metrics

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Whole course management of laparoendoscopic surgery for cervical cancer CHEN Changxian, LI Li 2019, 11 (3):  212-215.  doi: 10.3969/j.issn.1674-5671.2019.03.06 Abstract ( 280 )   PDF (1728KB) ( 469 )   Save Related Articles | Metrics

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Application of indocyanine green for sentinel lymph node mapping in laparoscopic fertility-sparing surgery for cervical cancer  LIU Qing, ZHU Hong, LIU Kaijiang, HU Zhijun 2019, 11 (3):  216-220.  doi: 10.3969/j.issn.1674-5671.2019.03.07 Abstract ( 460 )   PDF (621KB) ( 355 )   Save Objective To explore the effect of  indocyanine green（ICG）for sentinel lymph node mapping in laparoscopic fertility-sparing surgery for early cervical cancer. Methods Clinical data of 24 patients which underwent laparoscopic radical trachelectomy and sentinel lymph node biopsy were retrospectively analyzed. All patients were treated with ICG mapping. The detection rates，lymph node distribution and quantity were analyzed. Results Twenty-four  patients were included. Intraoperative ICG mapping detected at least one SLN in 24 patients （24/24），while bilateral mapping was achieved in 93.8% of cases（22/24）. There was no difference in detection rates between different injection method and patients with and without prior conization（P＞0.05）.A total of 210 SLN were resected in 24 patients，with an average of 8.75 SLN per patient（3-19 SLN）. 139 SLN are located in the iliac vessels，accounting for 66.19% （139/210）. Conclusions Sentinel lymph node mapping can be effectively applied to laparoscopic fertility-sparing surgery for early cervical cancers with strict indication and operational procedures. Related Articles | Metrics

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Effects of pioglitazone on proliferation and apoptosis of human pancreatic cancer cell line PANC-1 and its molecular mechanism LI Junling, ZHANG Jingyi, KAN Fanggong, ZHAI Aidi, HAO Ying 2019, 11 (3):  221-227.  doi: 10.3969/j.issn.1674-5671.2019.03.08 Abstract ( 310 )   PDF (10434KB) ( 72 )   Save Objective  To investigate the effects of pioglitazone on the proliferation and apoptosis of human pancreatic cancer cell line PANC-1 and its molecular mechanism. Methods Human pancreatic cancer cell line PANC-1 was cultured in vitro，pioglitazone （0，10，20，50 μmol/L） or gemcitabine（50 μmol/L） was administered for 0 h，12 h，24 h，48 h　and 72 h，respectively. The proliferation of PANC-1 cells was at 0 h，12 h，24 h，48 h and 72 h detected by CCK-8 assay，cell cycle，and apoptosis were detected by flow cytometry at 72 h，4，6-diamidino-2-phenylindole（DAPI） staining was used to detect the morphological changes of apoptosis at 48 h，Western blot was used to detect apoptosis and the expression of MAPK/ERK signaling pathway-related proteins at 48 h. Results Compared with pioglitazone at 0 μmol/L，OD value of PANC-1 cells in 50 μmol/L gemcitabine，10，20 and 50 μmol/L pioglitazone groups at 24 h，48 h and 72 h decreased significantly（P<0.05），and the percentage of G0/G1 phase cells，the apoptotic rate，the expressions of Bax and caspase-3、p-ERK proteins increased significantly at 48 h（P<0.05），while the proportion of S phase cells，the expressions of Bcl-2 protein decreased significantly（P<0.05） at 48 h，the degree of cell shrinkage gradually increased，and the nucleus gradually fragmented. Compared with the 50 μmol/L gemcitabine group，the OD values of PANC-1 cells in the 10 μmol/L pioglitazone group was increased（Ｐ＜0.05），while the 50 μmol/L pioglitazone group was decreased at 24 h，48 h and 72 h（P<0.05）；the proportion of G0/G1 cells，the apoptotic rate，the expressions of Bax and caspase-3,p-ERK proteins were decreased（P<0.05），while the 50 μmol/L pioglitazone group was increased at 48 h（P<0.05）；the proportion of cells in S phase，the expressions of Bcl-2 protein were increased（P<0.05），while the 50 μmol/L pioglitazone group was decreased at 48 h（P<0.05）. Conclusions Pioglitazone can inhibit the proliferation and induce apoptosis of pancreatic cancer cell PANC-1，and it may be achieved by up-regulating the MAPK/ERK pathway. Related Articles | Metrics

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Research of TBX15 promoter methylation on its expression and functional in hepatocellular carcinoma PANG Ting, LIU Shun, QIU Xiaoqiang, LI Shu, QIN Xiaoling, LI Kehua, LIU Meiliang, WU Liuyu, ZENG Xiaoyun 2019, 11 (3):  227-232.  doi: 10.3969/j.issn.1674-5671.2019.03.09 Abstract ( 362 )   PDF (5122KB) ( 442 )   Save Objective To investigate the promoter methylation and expression level of TBX15 gene in hepatoma cells，and explore the effect of promoter methylation on its mRNA expression and cell tumor behavior. Methods Bisulfite sequencing PCR （BSP） and quantitative real-time PCR（qPCR） were used to detect the promoter methylation and expression level of TBX15 in three hepatocellular carcinoma cell lines（HepG2，MHCC97H，SNU449）. Then the blank plasmid，empty plasmid pc3.1 and TBX15 overexpression plasmid were transfected into the hepatoma cell SNU449，and the proliferation and apoptosis in three different groups of hepatoma cells were detected by CCK-8 assay and flow cytometry. Results Among the three hepatocellular carcinoma cell lines，the TBX15 gene had the highest promoter methylation rate（89.5%） and the lowest mRNA expression level in SNU449 hepatoma cell. The overexpression plasmid group proliferating for hepatoma cells after culturing 48 h was higher than that of the empty plasmid group. The difference was statistically significant（0.549±0.080 vs 0.457±0.506，P=0.015）. The results of total apoptosis counting showed that the overexpression plasmid group had a higher effect on the apoptosis ability of hepatoma cells than the blank plasmid group. The difference was statistically significant［（5.12±1.42）% vs （2.16±0.41）%，P=0.014］. Conclusions Abnormal methylation in the promoter region may be the main reason for the inactivation of TBX15 gene，and it was closely related to the malignant biological behavior of hepatocellular carcinoma. It was a predictor for the progression of hepatocellular carcinoma. Related Articles | Metrics

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Effect of silencing ERK5 on the biological function of gastric cancer SGC-7901 and BGC-823 cells PANG Sen, WU Xianghua, LI Guibin, CHEN Jun, HUANG Jingle 2019, 11 (3):  233-238.  doi: 10.3969/j.issn.1674-5671.2019.03.10 Abstract ( 304 )   PDF (47303KB) ( 67 )   Save Objective To investigate the effects of extracellular signal regulated kinase 5 （ERK5） on the biological functions of gastric cancer SGC-7901 and BGC-823 cells. Methods Quantitative real-time PCR（qRT-PCR） was used to detect the expression levels of ERK5 mRNA in　human gastric cancer cell lines and human gastric mucosal epithelial cell lines.The expression of ERK5 in gastric cancer cells SGC-7901 and BGC-823 was silenced by short hairpin RNA（shRNA） interference technology.After the ERK5 gene was knocked down in gastric cancer cells，cell growth and proliferation were detected by CCK-8 assay.　Cell colony formation ability　was detected by plate cloning assay，cell invasion and migration ability were detected by Transwell assay，cell apoptosis and cell cycle were detected by flow cytometry. Results Compared with GES-1 in gastric mucosal epithelial cells，ERK5 mRNA was highly expressed in gastric cancer cells SGC-7901，BGC-823，AGS，and HGC-27，the difference folds were 2.696±0.501，1.865±0.185，1.793±0.137 and 1.530±0.093，respectively（P<0.05）. ERK5-shRNA effectively silenced the expression of ERK5 in gastric cancer cells SGC-7901 and BGC-823，and the silencing efficiency were（74.4±1.5）% and （69.1±3.9）%，respectively （P<0.05）. Silencing ERK5 expression could effectively inhibit the proliferation，colony formation，migration and invasion of gastric cancer cells（P<0.05）.Silencing ERK5 expression increased gastric cancer cell apoptosis rate（P<0.05），and induced cell cycle was arrested at the G0/G1 phase. Conclusions Silencing ERK5 significantly inhibit the proliferation and invasion of gastric cancer cell lines SGC-7901 and BGC-823.ERK5 may become a potential target for the treatment of gastric cancer in the future. Related Articles | Metrics

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Prognostic value of peripheral blood platelet to lymphocyte ratio for follicular lymphoma QUAN Xiaoying, YE Bin, WU Chunzhi, LEI Lei, CHEN Xiaoyan, ZHANG Zhihui 2019, 11 (3):  239-244.  doi: 10.3969/j.issn.1674-5671.2019.03.11 Abstract ( 359 )   PDF (1642KB) ( 502 )   Save Objective  To explore the prognostic value of platelet to lymphocyte ratio（PLR） for follicular lymphoma（FL）. Methods The clinical data of 74 patients with FL admitted to Sichuan Cancer Hospital from February 2006 to December 2016 were retrospectively analyzed. The cutoff value of PLR was calculated by X-Tile 3.6.1 software，and the patients were divided into low PLR group（<186.8，n=59）and high PLR group（≥186.8，n=15） according to the cutoff value. Cox regression analysis was used to analyze the relationship between PLR and prognosis. Results The median follow-up of this study was 42 months（range：6-144 months），4 years of progression free survival （PFS） was 42.0%，and overall survival （OS） was 54.7%. The 4-year PFS of the low PLR group was longer than that of the PLR group（51.3% vs 6.7%，χ2=25.320，P<0.001），and the 4-year OS was also longer than the PLR group（65.7%  vs 8.3%， χ2=29.414，P<0.001）. Multivariate Cox regression analysis showed that PLR>186.8 was an independent risk factor for PFS（HR=3.949，95%CI：1.856-8.404，P<0.001） and OS（HR=4.795，95%CI：2.259-10.178，P<0.001）for FL patients. Further analysis demonstrated that in low risk FL patients group，median PFS and median OS of the low PLR group were significantly longer than those of the high PLR group（PFS：75.5 months vs 19.0 months，χ2=14.798，P<0.001；OS：85.5 months vs 28.0 months，χ2=13.271，P<0.001）. Similarly，in intermediate-high risk FL patients group，median PFS and median OS of the low PLR group were also significantly longer than those of the high PLR group （PFS：32.0 months vs 12.0 months，χ2=10.766，P=0.001；OS：51.0 months vs 19.0 months，χ2=13.404，P<0.001）. Conclusion PLR is associated with prognosis in patients with FL and can be used as an indicator of prognosis. Related Articles | Metrics

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Prognostic impact of high body mass index on gastric cancer patients undergoing gastrectomy XIE Guisheng, WEI Haotang, CHEN Feng, NI Min, GAO Shunhui, CHEN Feng 2019, 11 (3):  245-251.  doi: 10.3969/j.issn.1674-5671.2019.03.12 Abstract ( 278 )   PDF (3459KB) ( 208 )   Save Objective To evaluate the effect of high body mass index（BMI） on the prognosis of gastric cancer patients undergoing gastrectomy. Methods　PubMed，EMBASE，Scopus，Cochrane Library，WanFang and CNKI databases were searched to find related studies on the effect of high BMI on the prognosis of patients that undergoing gastrectomy for gastric cancer from inception to December 31，2018. According to the inclusion and exclusion criteria，screened the literature，extracted data and evaluated the quality. Meta-analysis was performed using Review Manager 5.3 software. Results A total of 35 literatures were included in the study，including 32 113 patients（11 356 females and 20 757 males） with primary gastric cancer. Meta-analysis showed that the risk of postoperative complications in patients with high BMI was significantly higher than that in patients with low BMI，whether it was open or laparoscopic gastrectomy（RR=1.29，95%CI：1.14-1.46，P<0.001；RR=1.24，95%CI：1.04-1.47，P=0.01）. The 5-year overall survival rate of patients with high BMI gastric cancer was lower than that in patients with low BMI gastric cancer（RR=0.87，95%CI：0.79-0.96，P=0.006），but high BMI had no significant effect on perioperative mortality in patients with gastric cancer（RR=0.79，95%CI：0.51-1.22，P=0.29）. Conclusions Patients with high BMI gastric cancer have a higher incidence of postoperative complications and a lower 5-year survival rate than those patients with low BMI gastric cancer，but no increase perioperative mortality. Related Articles | Metrics

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The study of cardiotoxicity of FOLFOX4 project on patients with advanced hepatocellular carcinoma  LIANG Xiuqun, SHEN Yongqi, HUANG Hansheng, ZHANG Qing, WANG Zhixiang, LIN Haiyong, XIE Huadong, KONG Xiangying, HAN Chaowen 2019, 11 (3):  252-256.  doi: DOI：10.3969/j.issn.1674-5671.2019.03.13 Abstract ( 174 )   PDF (675KB) ( 242 )   Save Objective  To compare the FOLFOX4 regimen and RALOX regimen in the treatment of cardiotoxicity induced by advanced hepatocellular carcinoma（HCC）. Methods A total of 121 patients with advanced HCC were enrolled in 7 hospitals，including 63 patients with FOLFOX4 regimen （observation group） and 58 patients with RALOX regimen （control group）. The incidence of cardiotoxicity was compared between the two groups. Results The cardiotoxicity of the observation group and the control group were mainly grade Ⅰ and grade Ⅱ，but the incidence of grade Ⅰ and grade Ⅱ cardiotoxicity in the observation group were significantly higher than those of the control group（19.0% vs 6.9%，P=0.049；14.3% vs 3.4%，P=0.038），and the total incidence of cardiotoxicity in the observation group was also higher than the control group（36.5% vs 10.3%，P=0.001）. The incidence of abnormal electrocardiograph in the observation group was significantly higher than that in the control group（36.5% vs 10.3%，P=0.001）. However，there were no significant difference in left ventricular ejection fraction and myocardial enzyme elevation between the two groups（P＞0.05）. The incidence of arrhythmia and myocardial ischemia in the observation group were both higher than those of the control group（31.7% vs 10.3%，P=0.004；20.6% vs 6.9%，P=0.030）. Conclusions Grade Ⅰ and grade Ⅱ cardiotoxicity in the FOLFOX4 regimen caused by advanced HCC are higher than the RALOX regimen，and mainly characterized by arrhythmia and myocardial insufficiency Related Articles | Metrics

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MR manifestation of different molecular subtypes of breast cancer WEN Jie, KANG Wenyan, DENG Wenming, YANG Qian, LIU Zhou, LI Li, WEI Sulan, ZHONG Yihong, LUO Dehong 2019, 11 (3):  257-261.  doi: 10.3969/j.issn.1674-5671.2019.03.14 Abstract ( 402 )   PDF (632KB) ( 380 )   Save Objective To explore the differences in magnetic resonance（MR） manifestation of different molecular subtypes of breast cancer. Methods MR images from 69 breast cancer patients were retrospectively analyzed. The morphological characteristics，early enhancement rate，time-signal intensity cure（TIC） and apparent diffusion coefficient（ADC） value of the breast cancer lesions with each molecular subtype were identified and assessed jointly. Results Among 69 cases of breast cancer，62 cases（76 lesions） showed mass-like enhancement lesions，including 8 cases of Luminal A breast cancer（11 lesions），35 cases of Luminal B breast cancer（44 lesions），9 cases of HER-2 overexpression breast cancer（11 lesions） and 10 cases of triple-negative breast cancer（10 lesions）. In mass-like enhancement lesions，the morphological difference of different molecular subtypes of breast cancer cells was statistically significant（χ2=17.006，P=0.002），and the proportion morphology of round of triple-negative breast cancer was significantly larger than the other three groups（40.00% vs 10.61%，χ2=6.390，P=0.033）. The marginal difference of different molecular subtypes of breast cancer cells was also statistically significant （χ2=25.502，P=0.001），and the proportion of circumscribed margin of triple-negative breast cancer was significantly greater than the other three groups（60.00% vs 10.61%，χ2=14.942，P=0.001）. There were no significant differences in internal enhancement characteristics，early enhancement rate，TIC type and ADC value among different molecular subtypes of breast cancer（P>0.05）. Conclusions Different molecular subtypes of breast cancer have some differences in MR manifestation，which may help distinguish between triple-negative breast cancer and non-triple-negative breast cancer. However，there is not currently possible to directly predict the molecular subtype of breast cancer based on MR manifestation.  Related Articles | Metrics

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Application of individualized analgesia with multi-disciplinary collaborative in invasive radical resection of esophageal cancer OU Yufeng, ZHAO Huihua, WANG Hao 2019, 11 (3):  262-266.  doi: 10.3969/j.issn.1674-5671.2019.03.15 Abstract ( 306 )   PDF (638KB) ( 323 )   Save Objective To explore the effect of individualized analgesia management model with multi-disciplinary collaboration in patients with esophageal cancer after radical resection. Methods Totals of 50 patients who underwent radical resection of esophageal cancer in our hospotal from September 2017 to March 2018 were selected as the control group，and 48 patients from April to September 2018 were selected as the observation group.The traditional analgesic model was used in the control group，while the individualized analgesia management mode of multi-disciplinary cooperation was adopted in the observation group.The rest pain and activity pain score，the first activity time after operation，the number of patient-controlled analgnesia（PCA） pressing，the pump pause rate，the pump renewing rate and the incidence of adverse reactions were compared between the two groups. Results There were no significant difference in the first rest pain score, the mean rest pain score，the maximum rest pain score and the first activity pain score between the two groups（P＞0.05）. However, the mean value and the maximum value of active pain score in the observation group were significantly lower than the control group （P<0.05）. During the use of PCA, the total numbers of PCA pressing，daily PCA pressing and pump renewing rate in the observation group were significantly higher than those in the control group（P<0.001）. However, there was no significant difference in the pump pause rate between the two groups（P>0.05）. The ambulation time of getting out of bed in the observation group was significantly earlier than that in the control group（P<0.05），but there was no significant difference in the incidence of adverse reactions between the two groups（P>0.05）.  Conclusion The individualized analgesia management model with multi-disciplinary collaboration can improve the compliance of patients with analgesic treatment，and effectively control active pain and accelerate recovery. Related Articles | Metrics

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Advances in research of autophagy regulator Ambra1 in tumors LI Zhen, WEI Qingjun 2019, 11 (3):  267-270.  doi: 10.3969/j.issn.1674-5671.2019.03.16 Abstract ( 364 )   PDF (645KB) ( 455 )   Save Related Articles | Metrics