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Chinese Journal of Oncology Prevention and Treatment

Table of Content

    25 August 2019, Volume 11 Issue 4 Previous Issue    Next Issue
    Development and prospect of Guangxi cancer prevention and control work
    CAO Ji, GE Lianying, YU Jiahua, RONG Minhua, LI Qiulin
    2019, 11 (4):  271-275.  doi: 10.3969/j.issn.1674-5671.2019.04.01
    Abstract ( 358 )   PDF (623KB) ( 646 )   Save
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    Progress in immunotargeting therapy for extranodal NK/T-cell lymphoma
    ZHOU Xuan, WANG Liang
    2019, 11 (4):  282-288.  doi: 10.3969/j.issn.1674-5671.2019.04.03
    Abstract ( 406 )   PDF (2036KB) ( 524 )   Save
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    Expression of MYC in diffuse large B-cell lymphoma and its clinical significance
    KE Qing, DUAN Ying, HUANG Wenqiang, TAN Xiaohong, CEN Hong
    2019, 11 (4):  289-296.  doi: 10.3969/j.issn.1674-5671.2019.04.04
    Abstract ( 694 )   PDF (14026KB) ( 80 )   Save
    Objective To explore the expression of MYC in diffuse large B-cell lymphoma （DLBCL） and its relationship with the prognosis of DLBCL. Methods The clinical data of 220 patients with DLBCL admitted to Guangxi Medical University Cancer Hospital from February 2011 to August 2018 were retrospectively analyzed. The expression of MYC protein was detected by immunohistochemistry，and to analyze the correlations of MYC protein with clinical pathological characteristics and prognosis. Datasets containing the survival data and gene expression data about DLBCL were collected by searching Gene Expression Omnibus（GEO） to analyze the expression of MYC gene and its relationship with prognosis. Results The positive rate of MYC protein expression in 220 cases of DLBCL tissues was 18.64%. The overall survival（OS） and progress-free survival （PFS） of patients with positive MYC protein expression were significantly shorter than those with negative MYC protein expression（P<0.001）. Subgroup analysis indicated that in germinal center B-cell-like（GCB） subtype，the OS of patients with positive MYC protein expression were obviously shorter than that of patients with negative MYC protein expression，showing a statistically significant difference （P<0.001）. In non-GCB subtype，the OS and PFS of patients with positive MYC protein expression were shorter than those of patients with negative MYC protein expression，showing statistically significant differences（P<0.001）. The result of  Cox proportional hazard regression model analysis suggested that MYC protein expression was an independent risk factor for the prognosis of DLBCL patients（P<0.001）. GSE10846 dataset in GEO was analyzed，and the result con-firmed that the OS of patients with high MYC gene expression was evidently shortened （P<0.01）. Conclusion Patients with high MYC expression have a poor prognosis.
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    Vitamin D inhibited breast cancer stem cells through Wnt/β-catenin signaling pathway
    SHA Yutong, HUANG Jiaming, LU Zhenping, LIANG Huan, ZHENG Zongmei, YANG Xuepan, SHI Hongfei, ZHU Mingming
    2019, 11 (4):  297-302.  doi: 10.3969/j.issn.1674-5671.2019.04.05
    Abstract ( 403 )   PDF (6011KB) ( 233 )   Save
    Objective To explore the effect of Vitamin D on the activity of breast cancer stem cells and its possible mechanism. Methods The effects of different concentrations（1 μmol/L， 5 μmol/L， 10 μmol/L and 20 μmol/L） of Vitamin D on the viability of breast cancer SUM159 cells were detected by MTT assay. The breast cancer stem cells CSCSUM159 were enriched in suspension culture in serum-free medium to analyze the effect of Vitamin D on the activity of breast cancer stem cells.　Western blot and Real-time qPCR were used to detect the expression of the breast cancer stem cell markers CD133， CD44， Oct-4 and Nanog，and Wnt/β-catenin signaling pathway-related genes p-GSK-3β， β-catenin， c-Myc and cyclin-D1. Results MTT assay showed that 1 μmol/L， 5 μmol/L， 10 μmol/L and 20 μmol/L Vitamin D inhibited the viability of breast cancer SUM159 cells significantly（P＜0.01）. Serum-free medium culture could effectively enrich breast cancer stem cells CSCSUM159. Vitamin D reduced the number and size of SUM159 tumorsphere. Western blot and Real-time qPCR showed that Vitamin D down-regulated the protein expression of CD133， CD44 and Nanog and inhibited the protein expression levels of p-GSK-3β， β-catenin and c-Myc，and down-regulated the mRNA expression of β-catenin，c-Myc and cyclin-D1 in breast cancer stem cell CSCSUM159（P＜0.05）. Conclusion Vitamin D can inhibit the activity of breast cancer stem cells， which may be related to its regulation of Wnt/β-catenin signaling pathway.
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    Effect of bone marrow X-linked kinase on proliferation of cervical cancer cells and its potential #br# #br# mechanism#br#
    LI Xiaolan, ZHAO Junda, MA Junqi
    2019, 11 (4):  303-307.  doi: 10.3969/j.issn.1674-5671.2019.04.06
    Abstract ( 272 )   PDF (1443KB) ( 331 )   Save
    Objective To study the role of bone marrow X-linked kinase（BMX） on proliferation of human cervical cancer cells and its potential mechanism. Methods Clinical specimens of 34 cases of normal cervix，25 cases of cervical cancer in situ and 52 cases of invasive cervical cancer were collected from 2013 to 2017. Expression of BMX in different specimens was detected by immunohistochemistry. BMX-TALEN recombinant plasmid was transfected to construct a knockdown BMX-expressing cervical cancer cell line HeLa-BMX+/-. Cervical cancer cells HeLa-BMX+/- and HeLa-wild-type cells were cultured in complete medium containing inhibitor MK-2206 and rapamycin，respectively，and the cells cultured in DMSO medium were used as control group. The cell viability was detected by MTT analysis. The expression of BMX，Akt，p-Akt，mTOR and p-mTOR were detected by Western blot. Results The results of immunohistochemistry showed that the positive rate of BMX in normal cervix，cervical cancer in situ and invasive cervical cancer was statistically significant（26.5% vs 68.0% vs 88.5%，χ2=34.804，P<0.001），and there were statistical differences between the two groups（P<0.005）. Low-expression BMX cervical cancer HeLa-BMX cells were successfully constructed. Western blot results showed that the expres-sion of BMX and p-Akt in HeLa-BMX+/- cells were lower than that in HeLa-wild-type cells（t=6.282，8.117，P<0.001），while the expression of total Akt was similar（t=2.035，P=0.126）. Compared with the control group，the expression of p-Akt and p-mTOR in HeLa-wild-type and HeLa-BMX+/- cells treated with MK-2206 and rapamycin were inhibited. Conclusion BMX can promote the proliferation of cervical cancer cells，which may be related to the inhibition of PI3K/AKT/mTOR signaling pathway.
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    Effect of miR-125b-5p on energy metabolism and proliferation of laryngeal squamous cell carcinoma#br#
    CHEN Yongguo, XING Jun
    2019, 11 (4):  308-312.  doi: 10.3969/j.issn.1674-5671.2019.04.07
    Abstract ( 251 )   PDF (1066KB) ( 377 )   Save
    Objective To investigate the effect of miR-125b-5p affecting energy metabolism and proliferation of laryngeal squamous cell carcinoma by down-regulating hexokinase 2. Methods LSCC cells were divided into miRNA-125b-5p transfection group and control group. The expression of miR-125b-5p in normal bronchial epithelial cell line and LSCC cell line was detected by RT-qPCR，proliferation and apoptosis of LSCC cells were detected by CCK-8 and flow cytometry，HK2 expression in LSCC after overexpression of miR-125b-5p was detected by Western blot，glucose consumption and lactic acid production by LSCC cells were determined by 3H-2DG and lactate colorimetry. Results RT-qPCR results showed that the expression of miR-125b-5p in LSCC cell line was lower than that in normal cell line（0.22±0.05 vs 0.68±0.03，t=7.025，P=0.001）. CCK-8 results showed that after 72 h and 96 h of transfection，the proliferation of LSCC cells in the miR-125b-5p transfection group was lower than that in the control group（P<0.05）. The results of flow cytometry showed that the apoptotic rate of LSCC cells in the miR-125b-5p transfection group was higher than that in the control group［（37.52±2.34）% vs （12.46±3.52）%，t=7.025，P<0.001）］. The colony-forming ability of LSCC cells in the miR-125b-5p transfection group was also lower than that in the control group（0.29±0.02 vs 1.02±0.03，t=5.689，P=0.005）. The results of luciferase reporter gene assay showed that the luciferase activity of the miR-125b?-5p transfection group was lower than that in the control group（0.32±0.03 vs 1.01±0.02，t=7.543，P=0.001）. The results of Western blot showed that the protein level of HK2 in LSCC cells over-expressed by miR-125b-5p in the miR-125b-5p transfection group was lower than that in the control group（0.12±0.02 vs 0.75±0.03，t=5.875，P=0.023）. The results of lactate colorimetriy assay showed that the glucose consumption of LSCC cells in the miR-125b-5p transfection group was lower than that in the control group［（3.85±0.86） dpm/mg vs （10.52±1.34） dpm/mg，t=6.118，P=0.005］. The production of lactic acid in LSCC cells transfected with miR-125b-5p was also lower than that in the control group［（4.23±1.36） dpm/mg vs （10.96±2.45） dpm/mg，t=5.907，P=0.002］. Conclusion miR-125b-5p may reduce the energy metabolism and proliferation of laryngeal squamous cell carcinoma by down-regulating HK2 expression, and induce apoptosis.
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    Expression  of lncRNA MEG3 and lncRNA GAS5 in serum of multiple myeloma and its clinical significance
    GAO Peng, XIAO Zhongping, FU Kun, FENG Tao, YANG Dan, HAN Mei
    2019, 11 (4):  313-318.  doi: 10.3969/j.issn.1674-5671.2019.04.08
    Abstract ( 368 )   PDF (2647KB) ( 324 )   Save
    Objective To investigate the expression of long non-coding RNA （lncRNA） maternally expressed gene 3 （MEG3） and growth arrest-specific transcript 5（GAS5） in serum of patients with multiple myeloma and their clinical significance. Methods A total of 87 patients with multiple myeloma admitted to our hospital from January 2014 to January 2016 were selected as the study subjects，and 54 healthy volunteers were selected as the control group. The expression levels of lncRNA MEG3 and lncRNA GAS5 were detected by real-time quantitative reverse transcription polymerase chain reaction（qRT-PCR），and their relationships with clinicopathological data and prognosis of patients were analyzed. Results The levels of serum lncRNA MEG3 and lncRNA GAS5 in multiple myeloma patients were lower than those in the control group（5.92±0.87 vs 9.13±1.26，t=17.882，P<0.001；4.27±0.51 vs 8.26±1.04，t=30.417，P<0.001）. The expression levels of them were selected correlated with age，D-S stage，number of malignant plasma cells，β2-MG level and hemoglobin content（P<0.05）. The 3-year overall survival rate of the low-expression group of MEG3 and GAS5 were lower than those of the high-expression group of MEG3 and GAS5（32.56% vs 56.82%，χ2=5.617，P=0.018； 32.43%  vs 52.00%，χ2=5.389，P=0.020）. Low expression of MEG3 and low expression of GAS5 were associated with poor prognosis in patients with multiple myeloma（HR=3.503，95%CI：1.517-8.091；HR=3.319，95%CI：1.438-7.661）. Conclusions lncRNA MEG3 and lncRNA GAS5 are lowly expressed in serum of patients with multiple myeloma，which are related to the poor prognosis of the patients. 
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    Effect of preoperative ultrasound-guided quadratus lumborum block on early recovery and T cell immune #br# #br# function in patients undergoing radical gastrectomy#br#
    ZHOU Bo, MAO Xiaoli, HU Lei
    2019, 11 (4):  318-322.  doi: 10.3969/j.issn.1674-5671.2019.04.09
    Abstract ( 252 )   PDF (719KB) ( 390 )   Save
    Objective To investigate the effect of preoperative ultrasound-guided quadratus lumborum block（QLB） on early recovery and T cell immune function in patients undergoing radical gastrectomy. Methods Ｔotals　of 68 patients who underwent radical gastrectomy in our hospital were selected as the study subjects from March 2017 to June 2018，and divided into observation group（n=34） and control group（n=34） according to random number table method. After anesthesia induction，the observation group received color Doppler ultrasound-guided bilateral QLB（ropivacaine hydrochloride injection），while the control group received saline injection only. The postoperative analgesia indexes，VAS scores，serum CD4+CD25+ T cells level and adverse reactions of the two groups were compared. Results The dosage of sufentanil，the times of pressing analgesic pump and the proportion of remedial analgesia in the observation group were lower than the control group（P<0.05），and the score of analgesic satisfaction was higher than that in the control group（P<0.05）. The VAS scores of resting and moving state at 2，6，12 and 24 h after operation in the observation group were lower than those in the control group（P<0.05）. The difference of CD4+CD25+ T cells content before and after operation in the observation group was higher than that in the control group（2.0±0.8 vs 1.1±0.6，t=5.248，P＜0.001）. The proportion of nausea and vomiting（5.9% vs 26.5%），and vertigo（2.9% vs 20.6%）after anesthesia in the observation group were lower than those in the control group（P<0.05）. Conclusions Preoperative ultrasound-guided  quadratus lumborum block can reduce the dosage of analgesic drugs in patients undergoing radical gastrectomy，improve the analgesic effect during and after operation，the satisfaction of pain after operation and T cell immune function and with less adverse reactions. 
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    Correlation between serum prealbumin and prognosis in patients with alpha-fetoprotein-negative #br# #br# hepatocellular carcinoma#br#
    WANG Duo, LIANG Xiayi, LIU Junjie, CHEN Jie, CHEN Miao, LI Hang
    2019, 11 (4):  323-326.  doi: 10.3969/j.issn.1674-5671.2019.04.10
    Abstract ( 443 )   PDF (885KB) ( 591 )   Save
    Objective To investigate the effect of preoperative serum prealbumin（PA） on the prognosis of patients with alpha-fetoprotein-negative hepatocellular carcinoma（AFP-NHCC）. Methods A retrospective cohort study method was performed to collect 118 AFP-NHCC patients from Guangxi Medical University Cancer Hospital from January 2014 to December 2015. According to the preoperative serum PA content demarcation value，118 AFP-NHCC patienbs were divided into low PA group（<170 mg/L） and high PA group（≥170 mg/L）. Cox regression analysis was used to analyze the relationship between preoperative serum PA level and prognosis of patients with AFP-NHCC. Results Preoperative serum PA levels were associated with tumor diameter，macrovascular invasion，Child-Pugh classification，and BCLC stage（P<0.05）. Kaplan-Meier survival analysis showed that the median survival time was lower in the low PA group than that in the high PA group（24.5 months vs 36.0 months， χ2=11.689，P=0.001）. Cox regression analysis showed that patients with AFP-NHCC in the low PA group had a higher risk of death than those in the high PA group（HR=2.779，95%CI：1.406-5.492，P=0.003）， and this relationship persisted after controlling for potential confounders. Conclusion Preoperative serum low PA levels are associated with poor prognosis in patients with AFP-NHCC and can be used as an indicator of prognosis.
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    Comparison of high frequency color Doppler ultrasound features between infiltrating ductal carcinoma #br# #br# and non-lactation mastitis#br#
    WU Xiuhua, SHI Yanyun, LIU Yingzi, WU Bobo, LI Xiaoqin, CHEN Weixian
    2019, 11 (4):  327-331.  doi: 10.3969/j.issn.1674-5671.2019.04.11
    Abstract ( 277 )   PDF (3240KB) ( 299 )   Save
    Objective To compare the features of high frequency color Doppler ultrasound between infiltrating ductal carcinoma and non-lactation mastitis. Methods A total of 116 non-lactation mastitis cases（71 ultrasound-diagnosed non-lactation mastitis and 45 non-lactation mastitis misdiagnosed as cancer） and 115 infiltrating ductal carcinoma cases were analyzed retrospectively to compare their ultrasound features. Results Non-lactation mastitis group showed regular shape，clear boundary，no echo area，and posterior echo enhancement，and the difference was statistically significant compared with the misdiagnosed group（P<0.05）. Compared to infiltrating ductal carcinoma group，non-lactation mastitis group displayed regular shape，clear boundary，less speckle strong echo，no echo area，less hyperechoic halo，posterior echo enhancement，and less axillary nodes（P<0.05）. Infiltrating ductal carcinoma group and misdiagnosed group showed similar ultrasound features including irregular shape，unclear boundary，heterogeneous echo，and enriched blood supply，but displayed statistically significant of speckle strong echo（χ2=30.805，P<0.001），hyperechoic halo（χ2=20.282，P<0.001），and posterior echo enhancement （χ2=6.442，P=0.011）. Conclusions Non-lactation mastitis is easily misdiagnosed as infiltrating ductal carcinoma diagnosis by high-frequency color Doppler ultrasound. Attention to the observation of spotted strong echo，high echo halo，and rear echo enhancement are helpful for its correct diagnosis.
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    The value of serum microRNA-1,LASP1,TAGLN2 and LGALS3BP in the diagnosis of esophageal cancer
    BAI Yunpeng, LIU Yang, FAN Xiaoxi
    2019, 11 (4):  332.  doi: 10.3969/j.issn.1674-5671.2019.04.12
    Abstract ( 322 )   PDF (3077KB) ( 203 )   Save
    Objective To explore the value of serum microRNA-1，LASP1，TAGLN2 and LGALS3BP in the diagnosis of esophageal cancer. Methods A total of 128 esophageal cancer patients（observation group） and 134 healthy controls （control group） collected from a third-class hospital in Shenyang from January 2012 to January 2016 were enrolled in this study. The protein expression levels of LASP1，TAGLN2，and LGALS3BP in serum were detected by ELISA，and the mRNA expression of microRNA-1 in serum was detected by PCR. The ROC curve was used to evaluate the diagnostic values of serum LASP1，TAGLN2，LGALS3BP and microRNA-1 in esophageal cancer. Results The protein expression levels of LASP1，TAGLN2 and LGALS3BP in the observation group were higher than those in the control group（P<0.001），but the expression level of microRNA-1 was lower than that in the control group（P<0.001）.In esophageal cancer patients，the serum LASP1，TAGLN2，and LGALS3BP were negatively correlated with microRNA-1（P<0.001）.  The AUC of serum microRNA-1，LASP1，TAGLN2 and LGALS3BP in the diagnosis of esophageal cancer were 0.940（95%CI：0.913-0.967），0.890（95%CI：0.843-0.936），0.841（95%CI：0.794-0.889） and 0.924（95%CI：0.893-0.956），and the AUC for combined diagnosis was 0.970（95%CI：0.954-0.987）. Conclusion The expression levels of LASP1，TAGLN2，LGALS3BP，and microRNA-1 in serum have diagnostic value for esophageal cancer. 
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    Research progress of Jagged1/Notch signaling pathway in colorectal cancer
    CHEN Kai, LIU Jing, CHEN Yiming, KANG Yue
    2019, 11 (4):  337-340.  doi: 10.3969/j.issn.1674-5671.2019.04.13
    Abstract ( 295 )   PDF (514KB) ( 399 )   Save
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    Research progress on clock genes and tumor metastasis
    MENG Xi, JIN Feng
    2019, 11 (4):  341-345.  doi: 10.3969/j.issn.1674-5671.2019.04.14
    Abstract ( 292 )   PDF (646KB) ( 424 )   Save
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    Research progress on  fat mass and obesity-associated and malignant tumors#br#
    TAN Aihua, LU Yongkui, XIE Weimin
    2019, 11 (4):  346-351.  doi: 10.3969/j.issn.1674-5671.2019.04.15
    Abstract ( 346 )   PDF (737KB) ( 422 )   Save
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    Non-secretory multiple myeloma with anaplastic plasmacytoma：a case report
    XIE Jing, GUAN Jun, YANG Hang, QIN Shuanglai, WANG Bing, HU Zuowei
    2019, 11 (4):  356-358.  doi: 10.3969/j.issn.1674-5671.2019.04.17
    Abstract ( 358 )   PDF (1628KB) ( 797 )   Save
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