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中国癌症防治杂志

中国癌症防治杂志 ›› 2015, Vol. 7 ›› Issue (6): 389-393.doi: 10.3969/j.issn.1674-5671.2015.06.01

• 基础研究 •    下一篇

复方化浊润燥降气方对小鼠食管癌癌前病变的干预作用

  

  1. 河北省中医院肿瘤二科；河北医科大学第四医院科研中心
  • 出版日期:2015-12-25 发布日期:2016-01-11
  • 通讯作者: 单保恩 keshi3456@163.com
  • 基金资助:

    河北省高层次人才资助项目（B2012003007）

Effects of Fufang Huazhuo Jiangqi on precancerous lesions of the esophagus in a mouse model

  • Online:2015-12-25 Published:2016-01-11

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被引次数

1

摘要：

目的 观察复方化浊润燥降气方对小鼠食管癌癌前病变的干预作用。方法 130只小鼠分为正常组、模型组、阳性对照组、中药预防组（简称“预防组”）、中药治疗组（简称“治疗组”），采用4-硝基喹啉-氧化物（4-NQO）制备小鼠食管癌癌前病变模型，用中药复方化浊润燥降气方进行干预，于用药第14周末及实验结束时用光镜、电镜观察各组小鼠食管上皮组织病理形态学变化。结果 模型组第14周末光镜下见小鼠上皮细胞体积增大，数量增多，细胞核增大，证实小鼠食管癌癌前病变模型制备成功。实验结束时癌变形成，各组癌变率正常组为0，模型组为80%、阳性对照组为21.4%、预防组为5.6%、治疗组为12.5%。电镜下见模型组细胞间隙增宽，桥粒减少，核固缩，染色质边移。光镜和电镜下均示阳性对照组、预防组、治疗组病理改变减轻。 结论 复方化浊润燥降气方能减轻小鼠食管癌前病变损害程度，对预防食管癌有一定作用。

关键词: 食管肿瘤, 食管癌癌前病变, 复方化浊润燥降气方, 病理形态学, 干预

Abstract:

Objective　To observe the effects of the Fufang Huazhuo Jiangqi traditional Chinese medicine preparation on a mouse model of precancerous esophageal lesions. Methods Mice were treated with 4-nitro quinoline-oxide（4-NQO）to induce formation of precancerous esophageal lesions，and the animals were distributed into normal group，model group，positive control group，prevention group，treatment group Esophageal epithelial tissue morphology was examined by light microscopy at 14 weeks of treatment，and by electron microscopy at the end of the experiment. Results The model group showed greater cell volume， cell number，and nuclear size at 14 weeks，confirming the successful induction of precancerous esophageal lesions. Malignant transformation occurred in none of the animals in the normal group，80% of animals in the model group，21.4% in the positive control group，5.6% in the prevention group，and 12.5% in the treatment group. At the end of the experiment，animals in the model group showed intercellular broadening，desmosomes，nuclear pycnosis，and chromatin edges. Fewer pathological changes were observed in the positive control，prevention and treatment groups. Conclusion The Fufang Huazhuo Jiangqi prescription may significantly protect against malignant transformation of precancerous esophageal injury in a mouse model. It may therefore be useful for preventing esophageal carcinoma in humans.

Key words: Ｅsophageal neoplasm, Esophageal precancerous, Fufang Huazhuo Jiangqi prescription, pathomorphology, Intervention