微信公众号

官网二维码
中国癌症防治杂志

中国癌症防治杂志 ›› 2017, Vol. 9 ›› Issue (3): 185-189.doi: 10.3969/j.issn.1674-5671.2017.03.05

• 基础研究 • 上一篇    下一篇

CD90和CD44分子在人肝癌组织中的表达

  

  1. 广西医科大学附属肿瘤医院实验研究部
  • 出版日期:2017-06-25 发布日期:2017-08-02
  • 通讯作者: 匡志鹏 kgzhou@163.com
  • 基金资助:

    广西肿瘤学优势特色重点学科资助项目（024012002）

Expression of CD90 and CD44 in human hepatoma tissues

  • Online:2017-06-25 Published:2017-08-02

PDF (PC)

301

赞

摘要：

目的  探讨肝癌干细胞标志物CD90和CD44分子在人肝癌组织中的表达。方法 采用实时荧光定量PCR和免疫组化法检测40例人肝癌组织及其40例相应远端癌旁组织中CD90和CD44基因mRNA和蛋白的表达。结果 CD90和CD44基因mRNA在肝癌组织中的表达高于远端癌旁组织,差异有统计学意义(P<0.05);CD90和CD44阳性分子定位于癌细胞膜与细胞浆,CD90和CD44细胞在肝癌组织中的阳性表达率分别为82.5%(33/40)和77.5%(31/40),在远端癌旁组织中分别为17.5%(7/40)和12.5%(5/40),两者差异均有统计学意义(P<0.05)。结论 CD90和CD44分子在人肝癌组织中高表达,但低表达于相应远端癌旁组织,CD90和CD44分子可能与肝癌的发生有关。

关键词: 肝肿瘤, 干细胞标志物, CD90, CD44

Abstract:

Objective To investigate differences in CD90 and CD44 expression between human hepatoma and distal liver. Method Expression of CD90 and CD44 was measured using quantitative real-time PCR in human hepatoma and distal liver tissue，and the expression of both proteins was detected by immunohistochemistry. Results Expression of CD90 and CD44 mRNA was significantly higher in human hepatoma than in distal liver （P<0.05）. CD90 and CD44 proteins localized to the membrane and cytoplasm of cancer cells. In human hepatoma，the CD90-positive rate was 82.5% （33/40） and the CD44-positive rate was 77.5%（31/40）；the correspond-ing values in distal liver tissue were significantly lower：17.5%（7/40） and 12.5%（5/40）（P<0.05）. Conclusion  Human liver cancer tissue shows high expression of CD90 and CD44，while distal liver tissue shows low expression. CD90 and CD44 may correlate signif-icantly with the incidence and development of liver cancer.

Key words: Liver neoplasms, Stem cell markers, CD90, CD44