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中国癌症防治杂志

中国癌症防治杂志 ›› 2017, Vol. 9 ›› Issue (3): 201-204.doi: 10.3969/j.issn.1674-5671.2017.03.08

• 临床研究 • 上一篇    下一篇

Caveolin-1在子宫内膜癌组织中的表达及临床意义

  

  1. 山东省德州市陵城区中医院妇产科
  • 出版日期:2017-06-25 发布日期:2017-08-02

Clinical significance of Caveolin-1 expression in endometrial carcinoma

  • Online:2017-06-25 Published:2017-08-02

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摘要：

目的　探讨窖蛋白-1（Caveolin-1）在子宫内膜癌组织中的表达及其与临床病理特征的关系。方法 采用免疫组化法及蛋白质印迹法检测45例子宫内膜癌组织（内膜癌组）、35例子宫内膜良性病变组织（良性组）和35例正常子宫内膜组织（正常组）中Caveolin-1蛋白的表达。实时荧光定量PCR法检测内膜癌组中Caveolin-1 mRNA的表达，并分析其与临床病理特征的关系。结果 Caveolin-1蛋白在内膜癌组、良性组、正常组中的表达呈递减趋势，差异有统计学意义（P<0.05）；在内膜癌组淋巴结转移者Caveolin-1 mRNA表达高于无淋巴结转移者（P=0.001）；TNM分期Ⅱ期、Ⅲ+Ⅳ期者高于Ⅰ期者（P=0.002，P=0.040）；低分化、中分化者显著高于高分化者（P＜0.001，P=0.020）；Caveolin-1在子宫内膜癌组织中的表达与年龄及组织分型无明显相关性（P＞0.05）。结论 Caveolin-1在子宫内膜癌组织中高表达，且与淋巴结转移、分化程度和ＴＮＭ分期有关，有望成为子宫内膜癌早期诊断的指标及治疗靶点。

关键词: 子宫肿瘤, 子宫内膜癌, Caveolin-1, 免疫组化

Abstract:

Objective To evaluate the expression of Caveolin-1 in endometrial carcinoma and investigate its association with pathological features. Methods Caveolin-1 expression was determined by immunohistochemistry and Western blot using rabbit polyclonal antibody in 45 cases of endometrial carcinoma，35 cases of hyperplastic endometrium，and 35 cases of normal endometrium.Caveolin-1 mRNA levels in endometrial carcinoma tissue were determined using real-time PCR. Results Immunohistochemistry and Western blot showed different rates of positive expression of Caveolin-1 in the different types of endometrium. Caveolin-1 expression tended to increase with degree of malignancy（P<0.05）.Among cases with endometrial carcinoma，Caveolin-1 expression was much higher in patients with lymph node metastasis （P=0.001），and it was higher in stages Ⅱ and Ⅲ+Ⅳ than in stage （P=0.002，P=0.040）. Caveolin-1 expression was much higher in moderate and low-grade endometrial carcinoma than in high-grade endometrial carcinoma （P＜0.001，P=0.020）. Conclusions Caveolin-1 expression is higher in endometrial carcinoma than in hyperplastic or normal endometrium，and it correlates significantly with tumor stage and lymph node metastasis.Caveolin-1 may play an important role in endometrial carcinoma progression.

Key words: Uterine neoplasms, Endometrial carcinoma, Caveolin-1, Immunohistochemistry