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中国癌症防治杂志

中国癌症防治杂志 ›› 2022, Vol. 14 ›› Issue (6): 611-616.doi: 10.3969/j.issn.1674-5671.2022.06.03

• 基础研究 • 上一篇    下一篇

驱动蛋白家族成员23对结直肠癌细胞增殖、迁移的影响及其作用机制

  

  1. 广西医科大学附属肿瘤医院实验研究部
  • 出版日期:2022-12-25 发布日期:2022-12-30
  • 通讯作者: 容敏华 E?mail：tourtair@163.com
  • 基金资助:
    广西自然科学基金项目（2020GXNSFAA259022）

Effect of kinesin family member 23 on the proliferation and migration of colorectal cancer cells and its mechanism

  • Online:2022-12-25 Published:2022-12-30

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摘要： 目的 探讨驱动蛋白家族成员23 （kinesin family member 23，KIF23）在结直肠癌中的表达及其在结直肠癌细胞中的生物学功能和可能的作用机制。 方法 收集2021年5月至2021年12月在广西医科大学附属肿瘤医院诊治的20例结直肠患者癌组织及其癌旁正常组织，采用RT⁃qPCR方法检测KIF23在结直肠癌组织中的表达，分析其与临床病理特征的关系，并利用在线数据库（TGGA、CCLE）进行表达差异的验证。使用siRNA转染技术沉默人结直肠癌RKO细胞中的KIF23后，分别采用CCK⁃8实验、EDU增殖实验、Transwell迁移实验检测细胞增殖、迁移能力，Western blot检测MDM2、p53、p21的表达情况。结果 KIF23在结直肠癌组织中的表达高于其癌旁正常组织（P<0.0001），在线数据库验证结果呈现相同趋势（P<0.0001）。单因素分析结果显示，KIF23表达水平与肿瘤转移、CEA水平及CA199水平相关（均P<0.05）。体外细胞实验结果显示，与siNC组相比，siKIF23组细胞的增殖、迁移能力均显著下降（均P<0.01），KIF23、MDM2蛋白表达水平降低（均P<0.01），p53、p21蛋白表达水平升高（均P<0.01）。结论 KIF23在结直肠癌组织中高表达，可能是通过MDM2⁃p53/p21信号通路调控结直肠癌细胞增殖、迁移等恶性行为。

关键词: 结直肠癌, 驱动蛋白家族成员23, MDM2, 增殖, 迁移

Abstract: Objective To investigate the expression of kinesin family member 23 (KIF23) in colorectal cancer, as well as its biological function and possible mechanism in colorectal cancer cells. Methods The cancer tissues and adjacent normal tissues from 20 colorectal cancer patients diagnosed and treated in Guangxi Medical University Cancer Hospital, from May 2021 to December 2021 were collected. The expression of KIF23 in colorectal cancer tissues was detected by RT⁃qPCR, its relationship with clinicopathological characteristics was analyzed, and the expression differences were verified by using an online database (TGGA，CCLE). KIF23 in human colorectal cancer RKO cells was silenced by using the siRNA transfection technology, cell proliferation and migration ability were detected by the CCK⁃8 assay, EDU proliferation assay, and Transwell migration assay. The expression levels of MDM2, p53, and p21 were detected by Western blot. Results The expression of KIF23 in colorectal cancer tissues was higher than that in adjacent normal tissues (P<0.0001), and the online database validation results showed the same trend (P<0.0001). Univariable analysis showed that expression level of KIF23 was correlated with tumor metastasis, CEA and CA199 levels (all P<0.05). The results of the vitro experiments showed that, compared with the siNC group, the proliferation and migration ability was significantly decreased in the siKIF23 group (all P<0.01), the protein expression levels of KIF23 and MDM2 were decreased (all P<0.01), and the protein expression levels of p53 and p21 were increased (all P<0.01). Conclusions KIF23 is highly expressed in colorectal cancer tissues, and may regulate malignant behaviors such as proliferation and migration of colorectal cancer cells through the MDM2⁃p53/p21 signaling pathway.

Key words: Colorectal cancer, Kinesin family member 23, MDM2, Proliferation, Migration

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