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中国癌症防治杂志

中国癌症防治杂志 ›› 2023, Vol. 15 ›› Issue (1): 25-30.doi: 10.3969/j.issn.1674-5671.2023.01.04

• 基础研究 • 上一篇    下一篇

EBV⁃miR⁃BART17⁃5p对鼻咽癌细胞增殖及迁移功能的调控及其作用机制

  

  1. 广西医科大学附属肿瘤医院消化肿瘤内科;广西医科大学第一附属医院学科建设办公室;广西医科大学附属肿瘤医院日间病房
  • 出版日期:2023-02-25 发布日期:2023-03-01
  • 通讯作者: 李永强 E-mail：lyq702702@126.com
  • 基金资助:
    国家自然科学基金项目（82002859）；广西自然科学基金项目（2020GXNSFBA297024；2020GXNSFBA297059）

Regulation of EBV⁃miR⁃BART17⁃5p on proliferation and migration of nasopharyngeal #br# carcinoma cells and its mechanism#br#

  • Online:2023-02-25 Published:2023-03-01

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摘要： 目的 探讨EB病毒（epstein⁃barr virus，EBV）编码的EBV⁃miR⁃BART17⁃5p对鼻咽癌细胞增殖及迁移的调控及其机制。方法 将慢病毒Vector control和 EBV⁃miR⁃BART17⁃5p mimic分别感染至HK1⁃EBV细胞，记为Vector control组和Mimic组，采用RT⁃qPCR验证慢病毒感染效果；EdU和CCK⁃8实验分别检测细胞的增殖能力；平板克隆形成实验检测细胞的集落形成能力；细胞划痕实验及Transwell 细胞迁移实验检测细胞的迁移能力；Western blot检测EBV⁃miR⁃BART17⁃5p靶基因PTEN的蛋白表达水平。结果 与Vector control组比较，Mimic组HK1⁃EBV细胞中EBV⁃miR⁃BART17⁃5p表达量显著升高（P<0.001），细胞增殖、集落形成和迁移能力均明显增强（均P<0.05），PTEN蛋白表达水平显著降低（P<0.001）。结论 EBV⁃miR⁃BART17⁃5p能调控鼻咽癌细胞HK1⁃EBV增殖和迁移，其作用机制可能与EBV⁃miR⁃BART17⁃5p反向调控肿瘤抑制因子PTEN表达有关。

关键词: 鼻咽癌, EBV-miR-BART17-5p, PTEN, 增殖, 迁移

Abstract: Objective To investigate the regulation of EBV⁃miR⁃BART17⁃5p encoded by epstein⁃barr virus (EBV) on the proliferation and migration of nasopharyngeal carcinoma cells and its mechanism. Methods The lentivirus  Vector control and EBV⁃miR⁃BART17⁃5p mimic were infected into HK1⁃EBV cells, and recorded as the Vector control group and the Mimic group, respectively. RT⁃qPCR was used to verify the infection efficiency of lentivirus. EdU and CCK⁃8 were used to detect the proliferation ability of cells. Colony formation ability of cells was detected by plate cloning assay. Cell migration ability was detected by cell scratch assay and Transwell assay. The protein expression of EBV⁃miR⁃BART17⁃5p target gene PTEN was detected by Western blot. Results Compared with the Vector control group, the expression of EBV⁃miR⁃BART17⁃5p in HK1⁃EBV cells of Mimic group was significantly increased (P<0.001), and the ability of cell proliferation, colony formation and migration were significantly enhanced (all P<0.05). The expression level of PTEN protein was significantly decreased (P<0.001). Conclusions EBV⁃miR⁃BART17⁃5p can regulate the proliferation and migration of HK1⁃EBV nasopharyngeal carcinoma cells, and the mechanism may be related to the reverse regulation of the expression of tumor suppressor PTEN by EBV⁃miR⁃BART17⁃5p.

Key words: Nasopharyngeal carcinoma, EBV-miR-BART17-5p, PTEN, Proliferation, Migration

中图分类号: 

  • R739.63