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中国癌症防治杂志

中国癌症防治杂志 ›› 2010, Vol. 2 ›› Issue (03): 169-171.doi:

• 论文 • 上一篇    下一篇

人鼻咽癌细胞CNE-2双向电泳样品制备方法的建立

黄诗婷;朱小东;曲颂;苏芳;王琪;张玮;   

  1. 广西医科大学研究生院;广西医科大学肿瘤医院放疗科;广西肿瘤防治研究所第六实验室;
  • 收稿日期:2010-09-25 修回日期:2010-09-25 出版日期:2010-09-25 发布日期:2010-09-25

Method of preparing sample for two dimensional electrophoresis from human nasopharyngeal carcinoma cell line CNE-2

HUANG Shi-ting,ZHU Xiao-dong1, QU Song1, et al.   

  1. (Graduate School,1Radiotherapy Department of Tumor Hospital,Guangxi Medical University, Nanning 530021, China
  • Received:2010-09-25 Revised:2010-09-25 Online:2010-09-25 Published:2010-09-25

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摘要： 目的建立和优化人鼻咽癌细胞CNE-2的蛋白质样品制备方法。方法采用3种不同方法提取CNE-2细胞的蛋白质,并进行双向凝胶电泳实验。结果采用通用裂解液裂解细胞,等电聚焦前在样品中加入Destreak试剂,可以获得图像清晰、分辨率高的双向电泳图谱。结论建立了适合于人鼻咽癌细胞CNE-2总蛋白样品的双向电泳方法,为后续比较蛋白质组学研究奠定基础。

关键词: 蛋白质提取, 双向电泳, 鼻咽癌细胞

Abstract: Objective To establish and optimize the method of preparing protein sample for two-dimensional electrophoresis (2-DE) form human nasopharyngeal carcinoma cell line CNE-2.Method Three different Methods of extracting protein from CNE-2 cells were adopted.Then the protein samples were separated by 2-DE.Result The method of combining general lysate and Destreak reagent made the 2-DE maps with clearer image and higher resolution than the other two methods.ConclusionsA method of preparing sample from CNE-2 cells for 2-DE is established, which lays a foundation for further studies on proteome.

Key words: Two dimensional electrophoresis, Nasopharyngeal carcinoma, Proteome