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中国癌症防治杂志

中国癌症防治杂志 ›› 2010, Vol. 2 ›› Issue (03): 172-175.doi:

• 论文 • 上一篇    下一篇

钙调蛋白S100 A9在肝细胞性肝癌中的表达及其意义

陈滨;彭民浩;李佳荃;蓝柳根;卢景宁;   

  1. 广西医科大学第一附属医院肝胆血管外科;
  • 收稿日期:2010-09-25 修回日期:2010-09-25 出版日期:2010-09-25 发布日期:2010-09-25

S100 A9 expression in hepatocellular carcinoma and its significance

CHEN Bin,PENG Min-hao,LI Jia-quan,et al.   

  1. (Department of Hepatobiliary & Vascular Surgery,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China
  • Received:2010-09-25 Revised:2010-09-25 Online:2010-09-25 Published:2010-09-25

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摘要： 目的通过比较蛋白质组学研究筛选到的差异蛋白S100 A9在不同HCC组织中的表达情况的观察,探讨其临床病理意义。方法应用免疫组织化学方法检测5例正常肝组织、15例乙肝后肝硬化组织和48例不同表型肝癌组织中S100 A9蛋白的表达,探讨其差异表达与临床病理之间的关系及其意义。结果 S100 A9蛋白主要定位在肝细胞细胞质内,在正常肝组织无阳性表达,15例乙肝后肝硬化组织中5例阳性,48例HCC组织中阳性率为33.3%,其中小肝癌未见阳性表达,侵袭转移性HCC的表达高于非侵袭HCC者(52.17%vs16%,P=0.019);EdmondsonⅢ级HCC的阳性表达明显高于EdmondsonⅡ级者(81.8%vs18.9%,P=0.000),与临床病理指标的相关性研究发现S100 A9与HCC侵袭、分化关系密切。结论 S100 A9蛋白在侵袭性HCC中表达明显上调,结合与临床病理资料相关性研究,结果表明S100 A9可能成为一种潜在的、有应用价值的HCC分类、侵袭预测的分子标志物。

关键词: 肝细胞性肝癌, 钙调蛋白S100 A9, 免疫组织化学

Abstract: Objective To investigate the differential expression of S100 A9 protein in different phenotype of human hepatocellular carcinoma (HCC) tissues,and to explore the clinicopathologic significance.MethodsExpressions of S100 A9 protein in different liver tissue specimens were observed by immunohistochemistry assay. The specimens included 5 cases of normal liver,15 cases of cirrhosis caused by hepatitis B,and 48 cases of HCC that were with different phenotypes. The relativity between the results of immunohistochemistry assay and the clinicopathologic parameters were analyzed.Results S100 A9 protein localized in hepatocellular cytoplasm. Expression of S100 A9 protein in normal liver tissue and small HCC was negative. One-third cirrhosis caused by hepatitis B and 16 cases (33.3%) of HCC were S100 A9 positive. The positive expression rate of invasive HCC was higher than that of non-invasive HCC (52.17% vs.16%,P=0.019),while the positive expression rate of grade III HCC by Edmondson-Steiner classification was significantly higher than that of grade II (81.8% vs.18.9%,P=0.000).Clinicopathologic correlation study found that S100 A9 expression was closely related to HCC invasion and differentiation.ConclusionsExpression of S100 A9 protein in invasive HCC increases significantly.S100 A9 may be a potential and valuable molecular marker for HCC classification and invasion prediction.

Key words: Human hepatocellular carcinoma, S100 A9, Immunohistrochemisty assay