关键词: 鼻咽肿瘤, 标本库, 组织, 血液, 经验

Abstract:

 Objective To describe the process and experiences of setting up a specimen bank of nasopharyngeal cancer （NPC） tumor tissue at Fujian Provincial Tumor Hospital as a resource for clinical and basic research. Methods Tissue and blood samples were collected from NPC patients and transported to the laboratory in a liquid nitrogen container and stored  at -196 ℃. Whole blood and separate fractions of plasma，serum，and buffy coat were prepared and stored at -80 ℃. Clinical and demographic data for patient tissue donors were recorded. Results The specimen bank contains 1，152 primary NPC tissue specimens，of which 1，151 are from primary malignant tumor while 1 is from a recurrent tumor；95 nasopharyngeal tissue samples from healthy control individuals；24 tissue samples from individuals with chronic NPC；and 2,416 samples of blood，plasma, buffy coat，and serum. Of the 2，416 blood samples，1，635 were collected before treatment and 781 were collected after treatment，making 499 matched pairs of pre-/post-treatment samples. There are 614 matched pairs of blood samples and tissues, among which there are 193 matched pairs of pre-/post-treatment blood samples. Conclusion An NPC specimen bank has been established at Fujian Provincial Tumor Hospital，and it is ready to provide material for research as well as to continue growing.

Key words: Nasopharyngeal neoplasm, Bank, Tissue, Blood, Experience