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中国癌症防治杂志

中国癌症防治杂志 ›› 2016, Vol. 8 ›› Issue (2): 83-87.doi: 10.3969/j.issn.1674-5671.2016.02.03

• 基础研究 • 上一篇    下一篇

miR-204对乳腺癌MCF-7细胞增殖及凋亡的影响

  

  1. 广西医科大学第一附属医院病理科；广西医科大学研究生学院
  • 出版日期:2016-04-25 发布日期:2016-05-13
  • 通讯作者: 冯振博 guanghu1963@126.com

Effect of miR-204 on proliferation and apoptosis of breast cancer cell line MCF-7

  • Online:2016-04-25 Published:2016-05-13

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摘要：

目的 探讨miR-204在人乳腺癌组织及MCF-7细胞中的表达水平及其对乳腺癌细胞增殖及凋亡的影响。方法 提取癌症和肿瘤基因组图谱（the cancer genome atlas，TCGA）数据库中浸润性乳腺癌的miR-204相关数据进行汇总，转染miR-204过表达病毒，筛选稳定转染细胞株并通过RT-qPCR检测转染率，MTT法检测过表达miR-204后乳腺癌MCF-7细胞的增殖情况，流式细胞仪检测细胞凋亡情况。 结果 miR-204在浸润性乳腺癌组织中的表达水平较癌旁组织明显下调（P＜0.001），且与淋巴结转移和远处转移相关（P＜0.05）。过表达miR-204能够抑制乳腺癌MCF-7细胞的增殖能力（P＜0.05）；miR-204上调后，细胞凋亡率达到（34.7±1.9）%，细胞凋亡能力明显增强（P＜0.001）。结论 miR-204可抑制乳腺癌细胞增殖并介导细胞凋亡。

关键词: 乳腺肿瘤, miR-204, 乳腺癌MCF-7细胞株, 增殖, 凋亡

Abstract:

Objective To explore the expression of miR-204 in human breast cancer tissues and the MCF-7 cell line，and to investigate the influence of miR-204 on proliferation and apoptosis of breast cancer cells. Methods The miR-204 sequence was cloned based on information in the TCGA database. then it was used to generate recombinant virus and than transfected into MCF-7 cell，and the expressed microRNA was detected in infected cells using RT-qPCR. The proliferative ability of infected cells expressing miR-204 was tested using the MTT assay. Flow cytometry analysis was used to determine extent of apoptosis. Results  Expression of miR-204 was significantly lower in invasive breast cancer tissues than in normal breast tissue；expression correlated with lymph node and cancer metastasis. MTT assays showed that overexpression of miR-204 inhibited MCF-7 cell proliferation，and flow cytometry suggested that it significantly increased the rate of apoptosis. Conclusion miR-204 can inhib it breast cancer cell proliferation and induce apoptosis.

Key words: Breast neoplasm, miR-204, Breast cancer cell line MCF-7, Proliferation, Apoptosis