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中国癌症防治杂志

中国癌症防治杂志 ›› 2017, Vol. 9 ›› Issue (4): 295-301.doi: 10.3969/j.issn.1674-5671.2017.04.10

• 鼻咽癌专栏 • 上一篇    下一篇

shRNA沉默Annxin A2基因对放射抗拒鼻咽癌细胞迁移和侵袭的影响

  

  1. 福建省肿瘤医院/福建医科大学附属肿瘤医院放射生物学及肿瘤放射治疗学研究室；福建省科技厅转化医学重点实验室
  • 出版日期:2017-08-25 发布日期:2017-09-12
  • 通讯作者: 苏颖。E-mail：zjsuying@hotmail.com
  • 基金资助:

    福建省科技计划资助项目（2014Y0014）；国家临床重点专科建设资助项目（国卫办医函〔2013〕544号）；福建省卫生厅临床重点专科（西医类别）建设资助项目（闽卫科教〔2012〕149号）

Effects of shRNA-mediated silencing of Annexin A2 on migration and invasion of radioresistant nasopharyngeal carcinoma cells

  1. Fujian Cancer Hospital & Fujian Medical University Cancer Hospital，Laboratory of Radiation Oncology and Radiobiology，Fujian Key Laboratory of Tumor Translational Cancer Medicine
  • Online:2017-08-25 Published:2017-09-12
  • Contact: Su Ying. E-mail：zjsuying@hotmail.com

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摘要：

目的 探讨shRNA沉默Annxin A2基因对放射抗拒鼻咽癌细胞迁移和侵袭的影响。方法 采用FuGENE HD将Annexin A2 shRNA转染放射抗拒的鼻咽癌CNE2（R743）细胞，qRT-PCR验证转染细胞中Annxin A2基因的表达，细胞划痕实验和Transwell实验观察下调Annxin A2基因表达及联合照射对鼻咽癌细胞迁移和侵袭的影响，Western blot检测细胞中基质金属蛋白酶2（MMP2）蛋白的表达。结果 转染组细胞Annexin A2 基因的mRNA相对表达量为（0.25±0.17），较对照组的（1±0.00）和转染对照组的（0.96±0.06）明显下降，差异有统计学意义（P<0.05）。细胞划痕实验显示，下调Annxin A2基因表达可抑制照射诱导的CNE2（R743）细胞的迁移能力（P<0.05）。Transwell实验结果显示，下调Annxin A2基因表达可抑制照射诱导的CNE2（R743）细胞的迁移和侵袭能力（P<0.05）。Western blot结果表明，下调Annxin A2基因表达能抑制照射诱导的MMP2蛋白的表达上调（P<0.05）。结论 下调Annxin A2基因表达能抑制照射诱导放射抗拒的鼻咽癌CNE2（R743）细胞的迁移和侵袭能力，可能与MMP2蛋白表达下调相关。

关键词: 鼻咽肿瘤, Annxin A2基因, 放射抗拒, 鼻咽癌CNE2（R743）细胞, 迁移, 侵袭

Abstract:

Objective To investigate the effect of shRNA-mediated silencing of Annexin A2 and combined irradiation on the migration and invasion of radioresistant nasopharyngeal carcinoma cells. Methods Annexin A2 shRNA was transfected into CNE2（R743） cells using FuGENE HD. The expression of Annexin A2 gene in transfected cells was verified by qRT-PCR. The effect of down-regulation of Annexin A2 expression combined with irradiation on the migration and invasion of nasopharyngeal carcinoma cells were determined by scratch assay and transwell chamber assay. The expression of MMP2 was detected by Western blot.  Results The relative expression of Annexin A2 mRNA in transfected group（0.25±0.17） was significantly lower than in control group（1±0.00） and transfected control group（0.96±0.06），the difference was statistically significant（P<0.05）. Cell scratches showed that down-regulation of Annexin A2 could inhibit the migration of CNE2（R743） cells induced by irradiation（P<0.05）. Transwell assay showed that down-regulation of Annexin A2 expression inhibited the migration and invasion of CNE2（R743） cells induced by irradiation（P<0.05）. Western blot analysis showed that down-regulation of Annexin A2 could inhibit the upregulation of MMP2 protein expression induced by irradiation（P<0.05）. Conclusion Down-regulation of Annexin A2 can inhibit the migration and invasion of CNE2（R743） cells induced by irradiation，which may be related to the down-regulation of MMP2 protein expression.

Key words: Nasopharyngeal neoplasm, Annexin A2 gene, Radioresistant, Nasopharyngeal carcinoma CNE2（R743） cells, Migration, Invasion