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中国癌症防治杂志

中国癌症防治杂志 ›› 2018, Vol. 10 ›› Issue (2): 128-132.doi: 10.3969/j.issn.1674-5671.2018.02.12

• 临床研究 • 上一篇    下一篇

非小细胞肺癌组织裸鼠皮下成瘤性与原发肿瘤临床病理特征的关系

  

  1. 广西医科大学附属肿瘤医院胸外科；广西医科大学研究生院；湖北省荆门市第一人民医院
  • 出版日期:2018-04-25 发布日期:2018-06-13
  • 通讯作者: 潘泓 E-mail：pan_hong1999@aliyun.com
  • 基金资助:

    广西医疗卫生适宜技术研究与开发资助项目（S201418-04）

Relationship between clinicopathological features of primary human non-small cell lung cancer and their tumorigenicity in nude mice

  • Online:2018-04-25 Published:2018-06-13

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摘要：

目的 探讨非小细胞肺癌（non-small cell lung cancer，NSCLC）组织在裸鼠皮下人源性移植瘤（patient- derived xenografts，PDX）模型的成瘤性及其与原发肿瘤临床病理特征的关系。方法 选取15例NSCLC组织移植于裸鼠皮下，观察成瘤情况，比较移植成瘤组和未成瘤组对应患者的临床病理特征，采用免疫组织化学法检测对应患者癌组织中p63、细胞角蛋白5/6（cytokeratin 5/6，CK5/6）、甲状腺转录因子-1（thyoid transcription factor-1，TTF-1）和Ki-67的表达。结果 15例NSCLC组织在裸鼠皮下移植后成瘤6例，成瘤率为40％。非小细胞肺癌 PDX模型的成瘤性与对应患者的TNM分期和肿瘤分化程度相关（P＜0.05）。成瘤组对应患者NSCLC组织中 TTF-1和 Ki-67蛋白阳性表达率高于未成瘤组（83% vs 22%；100% vs 22% ，P＜0.05），p63蛋白阳性表达率则低于未成瘤组（50% vs 100%，P=0.041）。结论 NSCLC组织在裸鼠皮下的成瘤性与对应患者癌组织中p63、TTF-1、Ki-67的表达水平有关，原发肿瘤的分化程度越低、分期越晚者在祼鼠皮下更易成瘤。

关键词: 肺肿瘤, 非小细胞肺癌, 人源性移植瘤, 免疫组织化学, 临床病理特征

Abstract:

Objective To investigate the relationship between the tumorigenicity of non-small cell lung cancer （NSCLC） tissue in a nude mouse model of patient-derived xenografts（PDX） and clinicopathological features of the primary tumor in the original patients. Methods A total of 15 NSCLC tissues were implanted subcutaneously in nude mice in order to assess their tumorigenicity，which was compared with the clinicopathologic features of the primary tumor. Expression of p63，cytokeratin 5/6 （CK5/6），thyroid transcription factor-1（TTF-1） and Ki-67 was detected by immunohistochemical analysis of tumor tissue from the original patients. Results Of the 15 NSCLC tissues transplanted into nude mice，6（40%） were tumorigenic. Tumorigenicity of the PDX model was related to TNM stage and tumor differentiation in the corresponding patients（P<0.05）. The rates of TTF-1 and Ki-67 expression in NSCLC tissue were significantly higher in the engrafted group than in the non-engrafted group （83% vs 22%，100% vs 22%，P<0.05），while the rate of p63 expression was significantly lower in the engrafted group （50% vs 100%，P=0.041）. Conclusions Expression intensity of p63，TTF-1，and Ki-67 in human NSCLC tissue correlates with its tumorigenicity in nude mice. Poorer differentiation of the primary tumor and more advanced stage correlate with greater tumorigenicity. 

Key words:  Lung neoplasms, Non-small cell lung cancer, Patient-derived xenografts, Immunohistochemical, Clinicopathological features