微信公众号

官网二维码
中国癌症防治杂志

中国癌症防治杂志 ›› 2019, Vol. 11 ›› Issue (4): 297-302.doi: 10.3969/j.issn.1674-5671.2019.04.05

• 基础研究 • 上一篇    下一篇

维生素D调控Wnt/β-catenin信号通路抑制乳腺癌干细胞活性

  

  1. 南京中医药大学第二临床医学院营养教研室
  • 出版日期:2019-08-25 发布日期:2019-09-17
  • 通讯作者: 朱明明 E-mail：mmzhu2015@126.com
  • 基金资助:
    国家自然科学基金项目（81803217）；江苏省高等学校自然科学研究项目（18KJB330003）

Vitamin D inhibited breast cancer stem cells through Wnt/β-catenin signaling pathway

  • Online:2019-08-25 Published:2019-09-17

PDF (PC)

233

赞

摘要： 目的 探讨维生素D对乳腺癌干细胞活性的影响及其可能的作用机制。方法 采用MTT法检测不同浓度（1 μmol/L、5 μmol/L、10 μmol/L和20 μmol/L）维生素D对乳腺癌SUM159细胞活力的影响，用无血清培养基悬浮培养富集乳腺癌干细胞CSCSUM159，分析维生素D对乳腺癌干细胞活性的影响。Western blot和Real-time qPCR 分别检测乳腺癌干细胞标志物CD133、CD44、Oct-4、Nanog以及Wnt/β-catenin信号通路相关分子p-GSK-3β、β-catenin、c-Myc和cyclin-D1的表达。结果 MTT法检测结果显示，1 μmol/L、5 μmol/L、10 μmol/L和20 μmol/L维生素D均可显著抑制乳腺癌SUM159细胞的活力（P＜0.01）。无血清悬浮培养可有效富集乳腺癌干细胞CSCSUM159。维生素D可使乳腺癌干细胞CSCSUM159的成球数量减少，且细胞成球大小较对照组小。Western blot和Real-time qPCR检测结果显示，维生素D干预后，乳腺癌干细胞CSCSUM159中CD133、CD44和Nanog的蛋白表达下调，Wnt/β-catenin信号通路相关分子p-GSK-3β、β-catenin和c-Myc蛋白表达及β-catenin、c-Myc和cyclin-D1 mRNA表达亦下调（P＜0.05）。结论 维生素D可抑制乳腺癌干细胞活性，可能与其调控Wnt/β-catenin信号通路活性有关。

关键词: 乳腺癌, 维生素D, 肿瘤干细胞, Wnt/β-catenin信号通路

Abstract: Objective To explore the effect of Vitamin D on the activity of breast cancer stem cells and its possible mechanism. Methods The effects of different concentrations（1 μmol/L， 5 μmol/L， 10 μmol/L and 20 μmol/L） of Vitamin D on the viability of breast cancer SUM159 cells were detected by MTT assay. The breast cancer stem cells CSCSUM159 were enriched in suspension culture in serum-free medium to analyze the effect of Vitamin D on the activity of breast cancer stem cells.　Western blot and Real-time qPCR were used to detect the expression of the breast cancer stem cell markers CD133， CD44， Oct-4 and Nanog，and Wnt/β-catenin signaling pathway-related genes p-GSK-3β， β-catenin， c-Myc and cyclin-D1. Results MTT assay showed that 1 μmol/L， 5 μmol/L， 10 μmol/L and 20 μmol/L Vitamin D inhibited the viability of breast cancer SUM159 cells significantly（P＜0.01）. Serum-free medium culture could effectively enrich breast cancer stem cells CSCSUM159. Vitamin D reduced the number and size of SUM159 tumorsphere. Western blot and Real-time qPCR showed that Vitamin D down-regulated the protein expression of CD133， CD44 and Nanog and inhibited the protein expression levels of p-GSK-3β， β-catenin and c-Myc，and down-regulated the mRNA expression of β-catenin，c-Myc and cyclin-D1 in breast cancer stem cell CSCSUM159（P＜0.05）. Conclusion Vitamin D can inhibit the activity of breast cancer stem cells， which may be related to its regulation of Wnt/β-catenin signaling pathway.

Key words: Breast cancer, Vitamin D, Cancer stem cells, Wnt/β?鄄catenin signaling pathway

中图分类号: 

  • R737.9