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中国癌症防治杂志

中国癌症防治杂志 ›› 2019, Vol. 11 ›› Issue (5): 382-386.doi: 10.3969/j.issn.1674-5671.2019.05.04

• 基础研究 • 上一篇    下一篇

广西HBV相关肝细胞癌及其癌旁组织中HBV S基因突变分析

  

  1. 广西医科大学；广西医科大学附属肿瘤医院实验研究部；3广西壮族自治区肿瘤防治研究所
  • 出版日期:2019-10-25 发布日期:2019-11-25
  • 通讯作者: 黄天壬 tianrenhuang@sina.com
  • 基金资助:
    国家自然科学基金项目（81660561）；广西研究生创新计划项目（YCSW2018109）

Analysis of HBV S gene mutation in HBV-related hepatocellular carcinoma tissues and pericarcinoma liver tissues in Guangxi

  • Online:2019-10-25 Published:2019-11-25

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摘要： 目的 了解广西乙型肝炎病毒（hepatitis B virus, HBV）相关肝细胞癌 (hepatocellular carcinoma，HCC)组织及其癌旁组织中HBV S基因突变分布状况。方法 收集2015—2016 年于广西医科大学附属肿瘤医院手术切除的43例HBV相关HCC患者癌组织及其癌旁组织。采用巢式PCR扩增HBV S基因片段并测序，Chromas和DNAMAN软件分析S基因序列。结果 肝细胞癌患者HBV S基因热点突变在癌组织及癌旁组织中的分布差异无统计学意义（P>0.05）；小分子蛋白（small hepatitis B surface antigen, SHBs）终止突变只存在于癌组织中，突变位点分别为sC69*、sW74*、sL94*、sW172*和sW182*。结论 广西HBV相关HCC癌组织及其癌旁组织HBV S基因热点突变分布无特异性，SHBs终止突变可能影响HCC发生发展。

关键词: 肝细胞癌, 乙型肝炎病毒, S基因, 突变

Abstract: Objective To investigate the distribution of hepatitis B virus（HBV） S gene mutation in hepatocellular carcinoma （HCC） and pericarcinoma liver tissues of Guangxi. Methods Totals of 43 patients with HBV-related HCC who underwent HCC surgery in the Guangxi Medical University Cancer Hospital，were selected as subjects from 2015 to 2016，and the HCC tissues and pericarci-noma liver tissues were collected. The HBV S gene fragments were amplified by nest PCR and sequenced. The sequence of S gene was analyzed by Chromas and DNAMAN software. Results There was no significant difference in the distribution of hot spot mutation of HBV S gene between HCC tissues and pericarcinoma liver tissues（P>0.05）. The stop mutation of small hepatitis B surface antigen （SHBs） were only found in cancer tissues， which were sC69*，sW74*，sL94*，sW172* and sW182*. Conclusions For HBV-related HCC in Guangxi，there is no specific distribution of hot spot mutations in HBV S gene  between cancer tissues and paracan-cerous liver tissues，SHBs stop mutation may affect the occurrence and development of HCC.

Key words: Hepatocellular carcinoma, Hepatitis B virus, S gene, Mutation

中图分类号: 

  • R735.7