关键词: 鼻咽癌, CNE2细胞, 汉黄芩素, 增殖, 凋亡

Abstract: Objective To investigate the effect of Wogonin on the proliferation and apoptosis of nasopharyngeal carcinoma cell line CNE2 and the related possible mechanism. Methods CNE2 cells were treated with Wogonin at different concentrations（2.5 μmol/L，5.0 μmol/L，10.0 μmol/L，and 20.0 μmol/L），with the solvent as the control group. The real-time unlabeled cell function analysis technique was used to monitor the proliferation of CNE2 cells，and the double fluorescent labeling method was used to detect the apoptosis of CNE2 cells，and Western blot was used to detect the protein expression of PCNA，Survivin，XIAP，and Bcl-2. Results Wogonin could inhibit the proliferation of CNE2 cells at different concentrations（2.5 μmol/L，5.0 μmol/L，10.0 μmol/L，20.0 μmol/L），and when treated for 24 h，the inhibition ratios were 20.3%，23.7%，33.4%，and 40.9%，respectively，and the IC₅₀ was 42.41 μmol/L； when treated for 36 h，the inhibition ratios were 18.0%，22.0%，36.1%，and 40.5%，respectively，and the IC₅₀ was 34.46 μmol/L； when treated for 48 h，the inhibition ratios were 7.4%，20.2%，35.0%，and 40.9%，respectively，and the IC₅₀ was 22.81 μmol/L. Compared to those of the solvent control group，the apoptosis rates of CNE2 cells treated with Wogonin（10 μmol/L，20 μmol/L） increased（t=23.710，P=0.001； t=43.934，P<0.001）. When CNE2 cells were treated with 20 μmol/L Wogonin for 48 hours，the expression of Bax protein increased significantly（P<0.005），whereas the expressions of Survivin，XIAP，and Bcl-2 proteins decreased（P<0.005）. Conclusion Wogonin can inhibit the proliferation and induce the apoptosis of nasopharyngeal carcinoma CNE2 cells，probably through promoting the expression of Bax protein while inhibiting the expressions of Survivin，XIAP，and Bcl-2 proteins.

Key words:  , Nasopharyngeal carcinoma；CNE2 cells；Wogonin；Proliferation；Apoptosis