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中国癌症防治杂志

中国癌症防治杂志 ›› 2020, Vol. 12 ›› Issue (4): 421-425.doi: 10.3969/j.issn.1674-5671.2020.04.10

• 基础研究 • 上一篇    下一篇

Linc00704对结直肠癌细胞增殖、迁移及侵袭的影响

  

  1. 空军军医大学唐都医院；空军军医大学肿瘤生物学国家重点实验室，空军军医大学西京消化病医院消化内科
  • 出版日期:2020-08-25 发布日期:2020-09-01
  • 通讯作者: 卢瑗瑗 E-mail：luyuandreamer@aliyun.com；王新 E-mail：wangx@fmmu.edu.cn
  • 基金资助:

    国家优秀青年科学基金项目（81822031）；国家自然科学基金项目（81972224；81871913；81673038）；科技部重点研发计划

    项目（2018YFC1313101）

Effects of Linc00704 on the proliferation,migration and invasion of colorectal cancer cells

  • Online:2020-08-25 Published:2020-09-01

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摘要：

目的 探讨Linc00704（long non-coding RNA 00704）对结直肠癌细胞增殖、迁移和侵袭能力的影响。方法 RT-qPCR检测配对结直肠癌及癌旁正常组织、结直肠癌细胞和正常永生化人结肠上皮细胞系FHC中Linc00704 mRNA的表达。反义寡核苷酸（antisenseoligonucleotide，ASO）转染结直肠癌细胞构建Linc00704低表达细胞模型，慢病毒感染结直肠癌细胞构建Linc00704过表达细胞模型，RT-qPCR检测转染效率；CCK-8实验检测细胞增殖能力，Transwell实验检测细胞迁移及侵袭能力。结果 相比癌旁组织，Linc00704在结直肠癌组织中表达降低（t=4.103，P<0.001）。8种结直肠癌细胞系中有6种细胞系（RKO、HCT15、NCI-H716、SW480、SW1463、SW48）Linc00704的表达较FHC细胞低（F=44.750，P<0.001）。沉默 Linc00704表达可促进Caco-2、DLD-1细胞迁移和侵袭能力（均P<0.01），但对增殖无明显影响（均P>0.05）。过表达Linc00704可抑制RKO、HCT15细胞迁移和侵袭能力（均P＜0.001），但对增殖无明显影响（均P>0.05）。结论 Linc00704在结直肠癌组织中呈低表达，过表达Linc00704可抑制结直肠癌细胞迁移和侵袭能力。

关键词: 结直肠癌, Linc00704, 增殖, 侵袭, 迁移

Abstract:

Objective To investigate the effect of Linc00704 （long non-coding RNA 00704） on the proliferation，migration and invasion ability of colorectal cancer cells. Methods RT-qPCR was used to detect the expression of Linc00704 mRNA in the matched colorectal cancer and adjacent normal tissues，colorectal cancer cells and normal immortalized human colon epithelial cell line FHC. A low-expressing cell model of Linc00704 was constructed by antisense oligonucleotide（ASO）transfecting colorectal cancer cells，and a cell model with overexpression of Linc00704 was constructed by lentivirus infecting colorectal cancer cells. The transfection efficiency was detected by RT-qPCR，the cell proliferation was detected by CKK-8 assay，and the cell migration and invasion were measured by Transwell assay. Results Compared with the adjacent tissues，the expression of Linc00704 decreased in colorectal cancer tissues（t=4.103，P<0.001）. Among the 8 colorectal cancer cell lines，the expression of Linc00704 in 6 cell lines（RKO，HCT15，NCI-H716，SW480，SW1463，SW48）was lower than that of FHC（F=44.750，P<0.001）. Silencing the expression of Linc00704 could promote the migration and invasion of Caco-2 and DLD-1 cells （all P<0.01），but had no significant effect on the proliferation （all P>0.05）. The overexpression of Linc00704 could inhibit the migration and invasion of RKO and HCT15 cells（all P<0.001），but had no effect on the proliferation（all P>0.05）. Conclusions Linc00704 is low-expressed in colorectal cancer tissues，and overexpression of Linc00704 can inhibit the migration and invasion ability of colorectal cancer cells.

Key words: Colorectal cancer, Linc00704, Proliferation, Invasion, Migration

中图分类号: 

  • R735.3+5