Abstract:

Objective To investigate the effect of c-jun silencing on the growth and angiogenesis of radioresistant human nasopharyngeal carcinoma CNE-2R cells implanted in nude mice. Methods Cells were infected with lentivirus expressing c-jun-targeting shRNA in CNE-2R cells  （c-jun-shRNA group） or with lentivirus expressing negative control shRNA （NC group）；control cells were left uninfected. Levels of vascular endothelial growth factor （VEGF） in the three types of cells were measured using Western blotting. Positive clones were selected and transplanted in nude mice. Levels of CD34 and VEGF were analyzed using immunochemistry. Results VEGF protein levels were significantly lower in the c-jun-shRNA group than in the NC and control groups in vitro and in vivo. In the c-jun-shRNA group，tumor volume was（720.85±72.10） mm³，tumor weight was （0.42±0.04） g，and MVD was 11.69±3.30. The corresponding results in the NC group were（1，196.81±90.69）mm³，（0.80±0.08） g，and 32.56±7.33；the corresponding results in the control group were （1，203.76±100.42） mm³，（0.83±0.05） g，and 35.45±4.87. Tumor volume，tumor weight，MVD and grading differed significantly between the c- jun- shRNA group and the other groups（P<0.05）. Conclusion Silencing the c- jun gene can inhibit the growth and angiogenesis of radioresistant nasopharyngeal carcinoma.

Key words: Nasopharyngeal neoplasms, c-jun gene, Xenograft nude mice, Xenografted tumor, Radioresistant, Angiogenesis