Abstract:

Objective To identify genetic risk factors for prostate cancer in the Zhuang minority by performing whole exome sequencing on prostate cancer tissues and adjacent tissues. Methods Cancer tissues and adjacent tissues were collected from 26 Zhuang individuals with prostate cancer. All exons were sequenced using the Illuminate platform. Mutated genes were screened，TOP 60 candidate genes were selected，and these candidates were examined for association with human disease based on analysis with the DAVID online function pathway enrichment database and an integrated genetic and clinical database for coding variants in the human genome. Results Co-mutation analysis showed that 23.1% of patients possessed a mutation in low-density lipoprotein receptor-related protein 1（LRP1）. An integrated database linking human genetic variation with clinical information showed that the LRP1 mutation at chr12：57556206 may be associated with prostate cancer. This analysis also identified five chr12 sites independent of 57556206 that may be related to prostate cancer： 57603908，57548363，57567636，57554683，and 57549979. Conclusion Mutations in the LRP1 gene may be related to prostate cancer in the Zhuang population.

Key words: Prostate neoplasms, Whole exome sequencing, Low-density lipoprotein receptor-related protein 1